A5062673132- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Photoreceptor and optogenetics research
- RNA Research and Splicing
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Pharmacogenetics and Drug Metabolism
- Bacteriophages and microbial interactions
- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Proteomics Techniques and Applications
- Redox biology and oxidative stress
- Hemoglobin structure and function
- Nitric Oxide and Endothelin Effects
- Modular Robots and Swarm Intelligence
- Diatoms and Algae Research
- Pharmaceutical Economics and Policy
- Sulfur Compounds in Biology
- Computational Drug Discovery Methods
- DNA Repair Mechanisms
- Muscle Physiology and Disorders
- Genomics and Phylogenetic Studies
- Micro and Nano Robotics
- Advanced biosensing and bioanalysis techniques
Institut de Biologie Physico-Chimique
2008-2025
Sorbonne Université
2008-2025
Centre National de la Recherche Scientifique
2011-2025
Université de Rouen Normandie
2023-2024
Centre Hospitalier Universitaire de Rouen
2023
Inserm
2023
Normandie Université
2023
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2018-2022
Université Paris Cité
2008-2016
Sorbonne Paris Cité
2011-2016
In Escherichia coli the glmS gene encoding glucosamine 6-phosphate (GlcN-6-P) synthase GlmS is feedback regulated by GlcN-6-P in a pathway that involves small RNA GlmZ. Expression of activated unprocessed form GlmZ, which accumulates when intracellular concentration decreases. GlmZ stabilizes transcript derives from processing. Overexpression second sRNA, GlmY, also activates expression an unknown way. Furthermore, mutations two genes, yhbJ and pcnB, cause accumulation full-length thereby...
Organelles are intracellular compartments which themselves compartmentalized. Biogenic and metabolic processes localized to specialized domains or microcompartments enhance their efficiency suppress deleterious side reactions. An example of intra-organellar compartmentalization is the pyrenoid in chloroplasts algae hornworts. This microcompartment enhances photosynthetic CO2-fixing activity Calvin-Benson cycle enzyme Rubisco, suppresses an energetically wasteful oxygenase mitigates limiting...
Summary The opportunistic pathogen P seudomonas aeruginosa PAO1 has a remarkable capacity to adapt various environments and survive with limited nutrients. Here, we report the discovery characterization of novel small non‐coding RNA : NrsZ (nitrogen‐regulated sRNA ). We show that under nitrogen limitation, is induced by NtrB / C two‐component system, an important regulator assimilation . aeruginosa's swarming motility, in concert alternative sigma factor RpoN Furthermore, demonstrate...
In the absence of telomerase, telomere shortening triggers DNA damage checkpoint and replicative senescence, a potent tumor suppressor mechanism. Paradoxically, this same process is also associated with oncogenic genomic instability. Yet, precise mechanism that connects these seemingly opposing forces remains poorly understood. To directly study complex interplay between dynamics instability, we developed system in Saccharomyces cerevisiae to generate track telomeres length telomerase. Using...
The ribosome-associated endoribonuclease Rae1 cleaves the Bacillus subtilis yrzI operon mRNA in a translation-dependent manner. This encodes up to four small peptides, S1027, YrzI, S1025, and S1024, whose functions are unknown. Here, we identified function of YrzI S1025 deciphered degradation pathways polycistronic mRNA. We show that is toxic at high concentrations, but co-expression with abolishes its toxicity, that, absence Rae1, major antidote YzI toxin. highly stable species containing...
Multicellular organisms implement a set of reactions involving signaling and cooperation between different types cells. Unicellular organisms, on the other hand, activate defense systems that involve collective behaviors individual organisms. In unicellular model alga Chlamydomonas (Chlamydomonas reinhardtii), existence function mechanisms in response to stress remain mostly at level formation small structures called palmelloids. Here, we report characterization mechanism abiotic can trigger...
Proteomic and transcriptomic technologies resulted in massive biological datasets, their interpretation requiring sophisticated computational strategies. Efficient intuitive real-time analysis remains challenging. We use proteomic data on 1417 proteins of the green microalga Chlamydomonas reinhardtii to investigate physicochemical parameters governing selectivity three cysteine-based redox post translational modifications (PTM): glutathionylation (SSG), nitrosylation (SNO) disulphide bonds...
Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination 5΄-tagRACE RNA-seq analyze total RNA content from wild-type strain poly(A)polymerase deleted mutant. A 178 transcripts were either up- or down-regulated mutant when compared strain. Poly(A)polymerase up-regulates expression all genes related FliA regulon several previously unknown transcripts, including numerous...
Polyadenylation is a universal post-transcriptional modification involved in degradation and quality control of bacterial RNAs. In Escherichia coli, it admitted that any accessible RNA 3' end can be tagged by poly(A) tail for decay. However, we do not have yet an overall view the population polyadenylated molecules. The sampling RNAs presented here demonstrates rRNA fragments tRNA precursors originating from internal spacer regions rrn operons, particular, rrnB are abundant polymerase...
The enzymatic system of cytochrome P450 (CYP450) is a group enzymes involved in the metabolism drugs present liver. Literature records instances underdosing due to concurrent administration another drug that strongly induces same for which first substrate and overdosing strong inhibition. IT solutions have been proposed raise awareness among prescribers mitigate these interactions. This study aimed develop interaction dashboard Cytochrome-mediated interactions (DIDC) using health care data...
The ribosome-associated endoribonuclease Rae1 cleaves the Bacillus subtilis yrzI operon mRNA in a translation-dependent manner. This encodes up to four small peptides, S1027, YrzI, S1025 and S1024, whose functions were unknown. We show that YrzI is toxic at high concentrations, but co-expression with abolishes its toxicity. highly stable species containing open reading frames results from endoribonucleolytic cleavage upstream of ORF followed by 5-exoribonucleolytic processing arrested...
Clopidogrel is one of the most prescribed antiplatelet drugs for cardiovascular diseases prevention. a prodrug metabolised by CYP2C19 in active metabolites with anti-aggregant effect. Pharmacogenetic variants such as CYP2C19*2 variant can alter effect clopidogrel leading to higher risk disease relapse. The aim this study was evaluate prescription characteristics pharmacogenetics patients on treatment, based specific query clinical data warehouse. We evaluated demographics patients,...
Summary Depending on their nature, living organisms use various strategies to adapt environmental stress conditions. Multicellular implement a set of reactions involving signaling and cooperation between different types cells. Unicellular the other hand must activate defense systems, which involve collective behaviors individual organisms. In unicellular model alga Chlamydomonas reinhardtii , existence function behavior mechanisms in response remain largely unknown. Here we report discovery...
Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI
Abstract DNA data storage is an emerging technology that has the potential to replace bulky, fragile and energy-intensive current digital media. Here, we report a strategy called Drive, organizes on long double stranded replicative molecules. The Drive unlimited capacity, its encoding scheme ensures biosafety of process by limiting sequence code for mRNA proteins. Using our approach, encoded two historical texts from French Revolution, Declaration Rights Man Citizen 1789 Woman Female...