A5004201895- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Insect and Pesticide Research
- Bioactive Natural Diterpenoids Research
- Probiotics and Fermented Foods
- Biochemical Analysis and Sensing Techniques
- Antimicrobial Peptides and Activities
- Biochemical and Molecular Research
- Insect symbiosis and bacterial influences
- Photosynthetic Processes and Mechanisms
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Entomopathogenic Microorganisms in Pest Control
- ATP Synthase and ATPases Research
- Escherichia coli research studies
- Plant-Microbe Interactions and Immunity
Plant & Food Research
2018-2024
University of Otago
2011-2013
University of Göttingen
2007-2010
Institut de Biologie Physico-Chimique
2008
Sorbonne Université
2008
Université Paris Cité
2008
Summary Amino sugars are essential precursor molecules for the biosynthesis of bacterial cell walls. Their synthesis pathway is initiated by glucosamine‐6‐phosphate (GlcN‐6‐P) synthase (GlmS) which catalyses rate limiting reaction. We report here that expression Escherichia coli glmS gene negatively feedback regulated its product GlcN‐6‐P at post‐transcriptional level. Initially, we observed mutants defective yhbJ , a rpoN operon, overproduce GlmS. Concomitantly, mRNA accumulates derived...
In Escherichia coli the glmS gene encoding glucosamine 6-phosphate (GlcN-6-P) synthase GlmS is feedback regulated by GlcN-6-P in a pathway that involves small RNA GlmZ. Expression of activated unprocessed form GlmZ, which accumulates when intracellular concentration decreases. GlmZ stabilizes transcript derives from processing. Overexpression second sRNA, GlmY, also activates expression an unknown way. Furthermore, mutations two genes, yhbJ and pcnB, cause accumulation full-length thereby...
ABSTRACT The Lia system, a cell envelope stress response module of Bacillus subtilis , is comprised the LiaRS two-component system and membrane-anchored inhibitor protein, LiaF. It highly conserved in Firmicutes bacteria, all orthologs investigated so far are activated by wall antibiotics. In to stress, systems cocci induce expression number genes that involved conferring resistance against its inducers. contrast, complete picture LiaR regulon B. still missing no phenotypes could be...
ABSTRACT Resistance of Enterococcus faecalis against antimicrobial peptides, both host origin and produced by other bacteria the gut microflora, is likely to be an important factor in bacterium's success as intestinal commensal. The aim this study was identify proteins with a role resistance model peptide bacitracin. Proteome analysis bacitracin-treated untreated cells showed that bacitracin stress induced expression cell wall-biosynthetic caused metabolic rearrangements. Among increased...
Maintaining envelope integrity is crucial for the survival of any bacterial cell, especially those living in a complex and ever-changing habitat such as soil ecosystem. The LiaRS two-component system part regulatory network orchestrating cell-envelope stress response Bacillus subtilis. It responds to perturbations cell envelope, presence antibiotics that interfere with lipid II cycle, bacitracin or vancomycin. LiaRS-dependent regulation strictly repressed by membrane protein LiaF absence...
Undecaprenyl pyrophosphate phosphatases (UppPs) have been implicated in bacitracin resistance some bacterial genera and the aim of this study was to determine role UppPs mediating low-level Enterococcus faecalis. The uppP gene identified genomes laboratory (JH2-2) clinical (V583) strains E. Gene fusions (uppP-lacZ) 5′-RACE were used expression. both inactivated mutants studied for antimicrobial susceptibility their susceptibilities various stress agents. UppP protein from faecalis showed...
The genes and molecular machines that allow for a thermoalkaliphilic lifestyle have not been defined. To address this goal, we report on the improved high-quality draft genome sequence of Caldalkalibacillus thermarum strain TA2.A1, an obligately aerobic bacterium grows optimally at pH 9.5 65 to 70°C wide variety carbon energy sources.
The aerobic thermoalkaliphile Caldalkalibacillus thermarum strain TA2.A1 is a member of separate order alkaliphilic bacteria closely related to the Bacillales order. Efforts relate genomic information this evolutionary ancient organism environmental adaptation have been thwarted by inability construct complete genome. existing draft genome highly fragmented due repetitive regions, and gaps between over regions were unbridgeable. To address this, Oxford Nanopore Technology's MinION allowed us...
A new dsRNA virus from the oomycete Phytophthora pluvialis has been characterized and designated as RNA 1 (PplRV1). The genome of PplRV1 reference is 6742 bp that encodes two predicted open reading frames (ORFs). ORF1 ORF2 overlap by a 47 nt "slippery" frameshift sequence. putative protein unknown function. shows high similarity to RNA-dependent polymerase (RdRp) other viruses. Phylogenetic analysis RdRp its most closely related viruses showed distinct known (below 33% amino acid...
Here, we report the draft genome sequence of "Candidatus Liberibacter europaeus" ASNZ1, assembled from broom psyllids (Arytainilla spartiophila) New Zealand. The assembly comprises 15 contigs, with a total length 1.33 Mb and G+C content 33.5%.
Austropuccinia psidii is the causal pathogen of myrtle rust disease Myrtaceae. To gain understanding initial infection process, gene expression in germinating A. urediniospores and Leptospermum scoparium-inoculated leaves were investigated via analyses RNA sequencing samples taken 24 48 h postinoculation (hpi). Principal component transformed transcript count data revealed differential between uninoculated L. scoparium control plants that correlated with three plant leaf resistance...
Following the detection of potato mop-top virus (PMTV) in New Zealand 2018, three near-complete PMTV genomes (AS22, AS99, AS144) were assembled from soil samples taken fields Canterbury. Phylogenetic analysis revealed that these form a distinct lineage, with limited genetic diversity, within species. This supports hypothesis share common origin, possibly resulting single (or limited) incursion into Zealand. A nucleotide polymorphism was identified region where key diagnostic primer binds....