A5035214960- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Biopolymer Synthesis and Applications
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
University of Göttingen
2007-2013
Helmholtz Centre for Infection Research
2010
Institut de Biologie Physico-Chimique
2008
Sorbonne Université
2008
Université Paris Cité
2008
Summary Amino sugars are essential precursor molecules for the biosynthesis of bacterial cell walls. Their synthesis pathway is initiated by glucosamine‐6‐phosphate (GlcN‐6‐P) synthase (GlmS) which catalyses rate limiting reaction. We report here that expression Escherichia coli glmS gene negatively feedback regulated its product GlcN‐6‐P at post‐transcriptional level. Initially, we observed mutants defective yhbJ , a rpoN operon, overproduce GlmS. Concomitantly, mRNA accumulates derived...
Bacterial small RNAs (sRNAs) are well established to regulate diverse cellular processes, but how they themselves regulated is less understood. Recently, we identified a regulatory circuit wherein the GlmY and GlmZ sRNAs of Escherichia coli act hierarchically activate mRNA glmS , which encodes glucosamine-6-phosphate (GlcN6P) synthase. Although two highly similar, only direct activator that base-pairs with mRNA, aided by protein Hfq. GlmY, however, does not bind Hfq activates indirectly...
In Escherichia coli the glmS gene encoding glucosamine 6-phosphate (GlcN-6-P) synthase GlmS is feedback regulated by GlcN-6-P in a pathway that involves small RNA GlmZ. Expression of activated unprocessed form GlmZ, which accumulates when intracellular concentration decreases. GlmZ stabilizes transcript derives from processing. Overexpression second sRNA, GlmY, also activates expression an unknown way. Furthermore, mutations two genes, yhbJ and pcnB, cause accumulation full-length thereby...
In Escherichia coli synthesis of glucosamine-6-phosphate synthase GlmS is feedback-controlled by a regulatory cascade composed small RNAs GlmY and GlmZ. When GlcN6P becomes limiting, accumulates inhibits processing Full-length GlmZ base-pairs with the glmS transcript activates GlmS, which re-synthesizes GlcN6P. Here we show that glmY expression controlled two overlapping promoters same transcription start site. A sigma(70)-dependent promoter contributes to during exponential growth....
Small RNAs GlmY and GlmZ compose a cascade that feedback-regulates synthesis of enzyme GlmS in Enterobacteriaceae. Here, we analyzed the transcriptional regulation glmY/glmZ from Yersinia pseudotuberculosis, Salmonella typhimurium Escherichia coli, as representatives for other enterobacterial species, which exhibit similar promoter architectures. The sRNAs Y. pseudotuberculosis are transcribed σ54-promoters require activation by response regulator GlrR through binding to three conserved...
The histidine protein (HPr) is the energy-coupling of phosphoenolpyruvate (PEP)-dependent carbohydrate:phosphotransferase system (PTS), which catalyzes sugar transport in many bacteria. In its functions, HPr interacts with a number evolutionarily unrelated proteins. Mainly, it delivers phosphoryl groups from enzyme I (EI) to sugar-specific transporters (EIIs). proteins different bacteria exhibit almost identical structures, and, where known, they use similar surfaces interact their target...