A5056997987- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- PI3K/AKT/mTOR signaling in cancer
- Metabolism, Diabetes, and Cancer
- RNA modifications and cancer
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Viral gastroenteritis research and epidemiology
- Sarcoma Diagnosis and Treatment
- Extracellular vesicles in disease
- Lubricants and Their Additives
- Molecular Biology Techniques and Applications
- Neuroblastoma Research and Treatments
- Immune cells in cancer
- Diabetes Treatment and Management
- Helicobacter pylori-related gastroenterology studies
- Microbial bioremediation and biosurfactants
- Enzyme Structure and Function
- Antibiotic Resistance in Bacteria
- Mitochondrial Function and Pathology
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Antimicrobial Resistance in Staphylococcus
Centre National de la Recherche Scientifique
2011-2022
Université Paris-Saclay
2018-2022
Institut Gustave Roussy
2018-2022
Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses
2018-2021
Université Paris-Sud
2019
Institut de Biologie Physico-Chimique
2011-2016
Sorbonne Paris Cité
2011-2016
Université Paris Cité
2011-2016
Expression Génétique Microbienne
2012-2013
Sorbonne Université
2011-2012
The low-density lipoprotein receptor-related protein LRP1 is a cell surface receptor with functions in diverse physiological pathways, including lipid metabolism. Here we show that LRP1-deficient fibroblasts accumulate high levels of intracellular cholesterol and cholesteryl-ester when stimulated for adipocyte differentiation. We demonstrate stimulates canonical Wnt5a signaling pathway prevents accumulation. Moreover, required lipolysis fatty acid synthesis independently the noradrenergic...
Clostridium difficile is an emergent human pathogen and the most common cause of nosocomial diarrhea. Our recent data strongly suggest importance RNA-based mechanisms for control gene expression in C. difficile. In effort to understand function RNA chaperone protein Hfq, we constructed characterized Hfq-depleted strain Hfq depletion led a growth defect, morphological changes, increased sensitivity stresses, better ability sporulate form biofilms. The transcriptome analysis revealed...
Abstract Charcot-Marie-Tooth disease type 1 A (CMT1A) lacks an effective treatment. We provide a therapy for CMT1A, based on siRNA conjugated to squalene nanoparticles (siRNA PMP22-SQ NPs). Their administration resulted in normalization of Pmp22 protein levels, restored locomotor activity and electrophysiological parameters two transgenic CMT1A mouse models with different severity the disease. Pathological studies demonstrated regeneration myelinated axons myelin compaction, one major step...
The low density lipoprotein receptor-related protein (LRP1) is a transmembrane receptor that integrates multiple signaling pathways. Its cytoplasmic domain serves as docking sites for several adaptor proteins such the Src homology 2/α-collagen (ShcA), which also binds to tyrosine kinase receptors insulin-like growth factor 1 (IGF-1) receptor. However, physiological significance of physical interaction between LRP1 and ShcA, whether this modifies signaling, are still unknown. Here we report...
Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination 5΄-tagRACE RNA-seq analyze total RNA content from wild-type strain poly(A)polymerase deleted mutant. A 178 transcripts were either up- or down-regulated mutant when compared strain. Poly(A)polymerase up-regulates expression all genes related FliA regulon several previously unknown transcripts, including numerous...
Polyadenylation is a universal post-transcriptional modification involved in degradation and quality control of bacterial RNAs. In Escherichia coli, it admitted that any accessible RNA 3' end can be tagged by poly(A) tail for decay. However, we do not have yet an overall view the population polyadenylated molecules. The sampling RNAs presented here demonstrates rRNA fragments tRNA precursors originating from internal spacer regions rrn operons, particular, rrnB are abundant polymerase...
A gene for the Hfq protein is present in majority of sequenced bacterial genomes. Its characteristic hexameric ring-like core structure formed by highly conserved N-terminal regions. In contrast, C-terminal forms an extension, which varies length, lacks homology, and predicted to be unstructured. Gram-negative bacteria, facilitates pairing sRNAs with their mRNA target thus affects expression, either positively or negatively, modulates sRNA degradation. Gram-positive its role still poorly...
The aim of the present study is to take advantage unique property polyisoprenoid chains adopt a compact molecular conformation and use these natural biocompatible lipids as nanocarriers drugs deliver siRNA. A new chemical strategy applied here conjugate squalene (SQ) solanesol (SOLA) siRNA consisting an activated variant azide–alkyne Huisgen cycloaddition also known copper-free (Cu-free) click chemistry. We conjugated against TMPRSS2-ERG, fusion oncogene found in more than 50% prostate...
The rpsO-pnp operon encodes ribosomal protein S15 and polynucleotide phosphorylase, a major 3′–5′ exoribonuclease involved in mRNA decay Escherichia coli . gene for the SraG small RNA is located between coding regions of rpsO pnp genes, it transcribed opposite direction relative to two genes. No function has been assigned SraG. Multiple levels post-transcriptional regulation have demonstrated operon. Here we show that new factor affecting expression. overexpression results reduction...
// Alice Gerges Geagea 1, 2 , Manfredi Rizzo 1 Abdo Jurjus Francesco Cappello 4 Angelo Leone Giovanni Tomasello Céline Gracia 3 Sahar Al Kattar Liliane Massaad-Massade and Assaad Eid Department of Internal Medicine, University Palermo, Italy Anatomy, Cell Biology Physiological Sciences, Faculty American Beirut, Lebanon Equipe Nouvelles Thérapies Anticancéreuses, UMR8203 CNRS, Gustave Roussy, Villejuif, France Biomedicine, Neurosciences Advanced Diagnosis, School Of Medicine Correspondence...
Bacterial small RNAs (sRNAs) play essential roles in the post-transcriptional control of gene expression. To improve their detection by conventional microarrays, we designed a custom microarray containing group probes targeting known and some putative Escherichia coli sRNAs. assess its potential sRNAs, RNA profiling experiments were performed with total extracted from E. MG1655 cells exponentially grown rich (Luria-Bertani) minimal (M9/glucose) media. We found that many sRNAs could yield...
Human TRIAP1 (TP53-regulated inhibitor of apoptosis 1; also known as p53CSV for p53-inducible cell survival factor) is the homolog yeast Mdm35, a well-known chaperone that interacts with Ups/PRELI family proteins and participates in intramitochondrial transfer lipids synthesis cardiolipin (CL) phosphatidylethanolamine. Although recent reports indicate prosurvival factor abnormally overexpressed various types cancer, knowledge about its molecular metabolic function human cells still elusive....
Recently, we detected a new fusion transcript LMO3-BORCS5 in patient with Ewing sarcoma within cohort of relapsed pediatric cancers. was as highly expressed the characteristic oncogene EWS/FLI1. However, expression level at diagnosis very low. Sanger sequencing depicted two variants leading to loss functional domain LIM2 LMO3 gene, and disruption BORCS5. In vitro studies showed that (i) increases proliferation, (ii) decreases apoptosis-related genes treatment sensitivity, (iii) downregulates...
<h3>Background</h3> Fc&x025B;R1γ-deficient Natural Killer (g-NK) cells are a naturally occurring subset of NK that occur in portion individuals latently infected with cytomegalovirus. The lack Fc&x025B;R1γ-adapter protein results exclusive signaling through CD3ζ and consequently, significantly enhanced CD16-mediated antibody-dependent cellular cytotoxicity (ADCC) compared to conventional (cNK) bulk coculture assays tumor cells. However, the resulting mechanisms underlying this ADCC...
Abstract Extracellular vesicles are lipid-enclosed released by most cells and present in all human bodily fluids. EVs carry genetic material proteins, which can be transferred to other cells, generating various biological effects. Caveolin-1 (Cav1) is a key structural protein of the small invaginations plasma membrane known as caveolae, function mechano-sensors tension buffering devices. Elevated levels Cav1 have been detected from patients with advanced cancer. Given importance mechanical...
Introduction Paediatric cancers (PC) represent the 1 st cause of death by disease in children as 20% patients still die from a recurrence malignancy. PC differ adult regarding their aetiology, cellular origin or mutational rate. Improving knowledge on biology relapsed tumours and identifying new targets are needed to provide treatments. Fusion genes responsible for ~20% consequence juxtaposition two previously separate which can activate proto-oncogenes inactivate tumour suppressor genes....