A5025875167- Metalloenzymes and iron-sulfur proteins
- RNA modifications and cancer
- Electrocatalysts for Energy Conversion
- Porphyrin Metabolism and Disorders
- Metal-Organic Frameworks: Synthesis and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Photosynthetic Processes and Mechanisms
- Polyoxometalates: Synthesis and Applications
- RNA and protein synthesis mechanisms
- Ammonia Synthesis and Nitrogen Reduction
- Inorganic Chemistry and Materials
- Biofuel production and bioconversion
- Folate and B Vitamins Research
- Ferrocene Chemistry and Applications
- Peroxisome Proliferator-Activated Receptors
- Algal biology and biofuel production
- Carbon dioxide utilization in catalysis
- Nanocluster Synthesis and Applications
- Cancer-related gene regulation
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Chemical Analysis and Environmental Impact
- Lipid metabolism and biosynthesis
- Redox biology and oxidative stress
- Hydrogen Storage and Materials
IFP Énergies nouvelles
2021-2024
Sorbonne Université
2017-2022
Centre National de la Recherche Scientifique
2009-2022
Laboratoire de chimie des processus biologiques
2017-2022
Collège de France
2017-2022
University of California, Davis
2021
Concordia University
2018
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2017
Centre for Plant Biotechnology and Genomics
2014-2017
Universidad Politécnica de Madrid
2014-2017
Bacterial and eukaryotic transfer RNAs have been shown to contain hypermodified adenosine, 2-methylthio-N(6)-threonylcarbamoyladenosine, at position 37 (A(37)) adjacent the 3'-end of anticodon, which is essential for efficient highly accurate protein translation by ribosome. Using a combination bioinformatic sequence analysis in vivo assay coupled HPLC/MS technique, we identified, from distinct signatures, two methylthiotransferase (MTTase) subfamilies, designated as MtaB bacterial cells...
Organelles are intracellular compartments which themselves compartmentalized. Biogenic and metabolic processes localized to specialized domains or microcompartments enhance their efficiency suppress deleterious side reactions. An example of intra-organellar compartmentalization is the pyrenoid in chloroplasts algae hornworts. This microcompartment enhances photosynthetic CO2-fixing activity Calvin-Benson cycle enzyme Rubisco, suppresses an energetically wasteful oxygenase mitigates limiting...
Significance Posttranscriptional modifications of tRNA are essential for translational fidelity. More specifically, mechanisms selective sulfuration tRNAs still largely unknown, and the enzymes responsible these reactions incompletely investigated. Therefore, characterizing such systems at molecular level is greatly valuable to our understanding a whole class modification reactions. We study TtuA, representative member enzyme superfamily, show that it intriguingly catalyzes nonredox sulfur...
Abstract Acyl-CoA:diacylglycerol acyltransferases 3 (DGAT3) are described as plant cytosolic enzymes synthesizing triacylglycerol. Their protein sequences exhibit a thioredoxin-like ferredoxin domain typical of class ferredoxins harboring [2Fe-2S] cluster. The Arabidopsis thaliana DGAT3 (AtDGAT3; At1g48300) is detected in germinating seeds. recombinant purified produced from Escherichia coli , although very unstable, exhibits DGAT activity vitro . A shorter version devoid its N-terminal...
Post-translational modifications of ribosomal proteins are important for the accuracy decoding machinery. A recent in vivo study has shown that rimO gene is involved generation 3-methylthio derivative residue Asp-89 protein S12 (Anton, B. P., Saleh, L., Benner, J. S., Raleigh, E. A., Kasif, and Roberts, R. (2008) Proc. Natl. Acad. Sci. U. S. A. 105, 1826-1831). This reaction formally identical to catalyzed by MiaB on C2 adenosine 37 near anticodon several tRNAs. We present spectroscopic...
NifB utilizes two equivalents of S-adenosyl methionine (SAM) to insert a carbide atom and fuse substrate [Fe-S] clusters forming the cofactor (NifB-co), which is then passed NifEN for further modification form iron-molybdenum (FeMo-co) nitrogenase. Here, we demonstrate that from methanogen Methanocaldococcus infernus radical SAM enzyme able reductively cleave 5'-deoxyadenosine competent in FeMo-co maturation. Using electron paramagnetic resonance spectroscopy have characterized three...
One of the main hurdles to engineer nitrogenase in a non-diazotrophic host is achieving NifB activity. an extremely unstable and oxygen sensitive protein that catalyzes low-potential SAM-radical dependent reaction. The product activity called NifB-co, complex [8Fe-9S-C] cluster serves as obligate intermediate biosyntheses active-site cofactors all known nitrogenases. Here we study diversity phylogeny naturally occurring proteins, their architecture functions distinct domains order understand...
Plant biomass can be converted to bioproducts using fungal enzymatic cocktails that have been improved by the implementation of lytic polysaccharide monooxygenases (LPMOs) known boost cellulases. However, due complexity their oxidative catalysis, it is still difficult properly control and sustain LPMO activity. Here, we investigated whether natural partner LPMOs, i.e., cellobiose dehydrogenase (CDH), could a promising solution. To this end, reconstituted minimal cocktail main cellulases from...
Abstract Lytic polysaccharide monooxygenases (LPMOs) are taxonomically widespread copper-enzymes boosting biopolymers conversion (e.g. cellulose, chitin) in Nature. White-rot Polyporales, which major fungal wood decayers, may possess up to 60 LPMO-encoding genes belonging the auxiliary activities family 9 (AA9). Yet, functional relevance of such multiplicity remains be uncovered. Previous comparative transcriptomic studies six Polyporales fungi grown on cellulosic substrates had shown...
[FeFe] hydrogenases are enzymes that have acquired a unique capacity to synthesize or consume molecular hydrogen (H
[FeFe] hydrogenases are highly active catalysts for the interconversion of molecular hydrogen with protons and electrons. Here, we use a combination isotopic labeling, 57Fe nuclear resonance vibrational spectroscopy (NRVS), density functional theory (DFT) calculations to observe characterize modes involving motion 2-azapropane-1,3-dithiolate (ADT) ligand bridging two iron sites in [2Fe]H subcluster. A −13C2H2– ADT labeling synthetic diiron precursor produced isotope effects observed...