A5068539394- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Heat shock proteins research
- Algal biology and biofuel production
- Photoreceptor and optogenetics research
- Microbial Metabolic Engineering and Bioproduction
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Microtubule and mitosis dynamics
- Analytical Chemistry and Sensors
- Plant Reproductive Biology
- Wastewater Treatment and Nitrogen Removal
- Literature, Culture, and Criticism
- Olfactory and Sensory Function Studies
- Mass Spectrometry Techniques and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Muscle activation and electromyography studies
- Plant Molecular Biology Research
- Architecture and Computational Design
- History and Theory of Mathematics
- 14-3-3 protein interactions
- Protist diversity and phylogeny
University of Kaiserslautern
2015-2023
Max Planck Institute of Molecular Plant Physiology
2021
University of Leicester
2011-2019
Humboldt-Universität zu Berlin
2014
École Polytechnique Fédérale de Lausanne
2009
Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, realize potential green cell factories, a major challenge is make microalgae easier engineer. A approach for rapid and predictable genetic manipulation use standardized synthetic biology tools workflows. To this end we have developed Modular Cloning toolkit the microalga Chlamydomonas reinhardtii. It...
Chloroplasts contain thousands of nucleus-encoded proteins that are imported from the cytosol by translocases in chloroplast envelope membranes. Proteolytic regulation is critically important, but little known about underlying mechanisms. We applied forward genetics and proteomics Arabidopsis to identify factors required for outer membrane (OEM) protein degradation. identified SP2, an Omp85-type β-barrel channel OEM, CDC48, a cytosolic AAA+ (ATPase associated with diverse cellular...
Stromal processing peptidase (SPP) is a metalloendopeptidase located in the stroma of chloroplasts, and it responsible for cleavage transit peptides from preproteins upon their import into organelle. Two independent mutant Arabidopsis lines with T-DNA insertions SPP gene were analysed (spp-1 spp-2). For both lines, no homozygous plants could be detected, segregating progeny spp heterozygotes contained heterozygous wild-type ratio 2∶1. The siliques spp-1 spp-2 many aborted seeds, at frequency...
Abstract In plant cells, chloroplast gene expression is predominantly controlled through post-transcriptional regulation. Such fine-tuning vital for precisely orchestrating protein complex assembly as the photosynthesis machinery and quickly responding to environmental changes. While regulation of synthesis central importance, little known about degree nature regulatory network, mainly due challenges associated with specific isolation transient ribosome interactors. Here, we established a...
In land plants and cyanobacteria, co-translational association of chlorophyll (Chl) to the nascent D1 polypeptide, a reaction center protein photosystem II (PSII), requires Chl binding complex consisting short-chain dehydrogenase (high fluorescence 244 [HCF244]/uncharacterized 39 [Ycf39]) one-helix proteins (OHP1 OHP2 in chloroplasts) light-harvesting antenna superfamily. Here, we show that an ohp2 mutant green alga Chlamydomonas (Chlamydomonas reinhardtii) fails accumulate core PSII...
Biochemical processes in chloroplasts are important for virtually all life forms. Tight regulation of protein homeostasis and the coordinated assembly complexes, composed both imported locally synthesized subunits, vital to plastid functionality. Protein biogenesis requires action cotranslationally acting molecular chaperones. One such chaperone is trigger factor (TF), which known bind most newly proteins bacteria, thereby assisting their correct folding maturation. However, how these...
Photosynthesis is a central determinant of plant biomass production, but its homeostasis increasingly challenged by heat. Little known about the sensitive regulatory principles involved in heat acclimation that underly biogenesis and repair chloroplast-encoded core subunits photosynthetic complexes. Employing time-resolved ribosome transcript profiling together with selective proteomics, we systematically deciphered these processes chloroplasts Chlamydomonas reinhardtii. We revealed protein...
To extend our understanding of chloroplast protein import and the role played by machinery component Tic40, we performed a genetic screen for suppressors chlorotic tic40 knockout mutant Arabidopsis thaliana plants. As result, two suppressor loci, stic1 stic2, were identified characterized. The locus corresponds to gene ALBINO4 (ALB4), which encodes paralog well-known thylakoid targeting factor ALB3. stic2 previously unknown stromal that interacts physically with both ALB4 Genetic studies...
Abstract ALBINO3 (ALB3) is a well-known component of thylakoid protein-targeting complex that interacts with the chloroplast signal recognition particle (cpSRP) and cpSRP receptor, filamentous temperature-sensitive Y (cpFtsY). Its protein-inserting function has been established mainly for light-harvesting proteins, which first interact unique cpSRP43 then are delivered to ALB3 integrase by GTP-dependent cpSRP-cpFtsY interaction. In Arabidopsis (Arabidopsis thaliana), subsequently discovered...