A5052981947- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Algal biology and biofuel production
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Neonatal Health and Biochemistry
- Pancreatic function and diabetes
- Agronomic Practices and Intercropping Systems
- Protist diversity and phylogeny
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Nitrogen and Sulfur Effects on Brassica
- Wheat and Barley Genetics and Pathology
- Micro and Nano Robotics
- Agriculture, Plant Science, Crop Management
- Plant Micronutrient Interactions and Effects
- Lipid metabolism and biosynthesis
- Erythrocyte Function and Pathophysiology
- Gene Regulatory Network Analysis
Max Planck Society
2024
Max Planck Institute of Molecular Plant Physiology
2015-2023
Summary The efficiency of gene expression in all organisms depends on the nucleotide composition coding region. GC content and codon usage are two key sequence features known to influence expression, but underlying molecular mechanisms not entirely clear. Here we have determined relative contributions nuclear unicellular green alga Chlamydomonas reinhardtii . By comparing variants that encode an identical amino acid differ their and/or usage, show is factor determining translational and,...
Silencing of exogenous DNA can make transgene expression very inefficient. Genetic screens in the model alga Chlamydomonas have demonstrated that silencing be overcome by mutations unknown gene(s), thus producing algal strains stably express foreign genes to high levels. Here, we show mechanism specifically acts on transgenic DNA. Once a permissive chromatin structure has assembled, persist even absence disrupting pathway. We identified gene conferring and it encode sirtuin-type histone...
The unicellular green alga Chlamydomonas reinhardtii has become an invaluable model system in plant biology. There is also considerable interest developing this microalga into efficient production platform for biofuels, pharmaceuticals, chemicals and industrial enzymes. However, the of foreign proteins nucleocytosolic compartment greatly hampered by inefficiency transgene expression from nuclear genome. We have recently addressed limitation isolating mutant algal strains that permit...
The inner-envelope K+ EFFLUX ANTIPORTERS (KEA) 1 and 2 are critical for chloroplast development, ion homeostasis, photosynthesis. However, the mechanisms by which changes in flux across envelope affect organelle biogenesis remained elusive. Chloroplast development requires intricate coordination between nuclear genome plastome. Many mutants compromised plastid gene expression (PGE) display a virescent phenotype, that is delayed greening. phenotypic appearance of Arabidopsis thaliana kea1...
Sulfur deficiency-induced proteins SDI1 and SDI2 play a fundamental role in sulfur homeostasis under sulfate-deprived conditions (-S) by downregulating glucosinolates. Here, we identified that besides glucosinolate regulation -S, downregulates another pool, the S-rich 2S seed storage Arabidopsis (Arabidopsis thaliana) seeds. We MYB28 directly regulates binding to At2S4 promoter. also showed forming ternary protein complex with MYC2, transcription factor involved of proteins. These findings...
Abstract Background Ribosome profiling (or Ribo-seq) is a technique that provides genome-wide information on the translational landscape (translatome). Across different plant studies, variable methodological setups have been described which raises questions about general comparability of data were generated from diverging methodologies. Furthermore, common problem when performing Ribo-seq are abundant rRNA fragments wastefully incorporated into libraries and dramatically reduce sequencing...
Abstract Ribosome profiling (or Ribo-seq) is a technique that provides genome-wide information on the translational landscape (translatome). Across different plant studies, variable methodological setups have been described which raises questions about general comparability of data were generated from diverging methodologies. Furthermore, common problem when performing Ribo-seq are abundant rRNA fragments wastefully incorporated into libraries and dramatically reduce sequencing depth. To...
In plant biotechnology and basic research, chloroplasts have been used as chassis for the expression of various transgenes. However, potential unintended side effects transgene insertion high-level on native chloroplast genes are often ignored not studied comprehensively. Here, we examined genome at both transcriptional translational levels in five transplastomic tobacco (Nicotiana tabacum) lines carrying identical aadA resistance marker cassette diverse genomic positions. Although none...
A Correction to this paper has been published: https://doi.org/10.1038/s41467-020-20623-0