A5004057624- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Mitochondrial Function and Pathology
- Vibrio bacteria research studies
- RNA Research and Splicing
- Bacterial biofilms and quorum sensing
- Aquaculture disease management and microbiota
- Salmonella and Campylobacter epidemiology
- ATP Synthase and ATPases Research
- Algal biology and biofuel production
- Light effects on plants
- Escherichia coli research studies
- CRISPR and Genetic Engineering
- Metalloenzymes and iron-sulfur proteins
Czech Academy of Sciences, Institute of Microbiology
2024
Max Planck Society
2024
Max Planck Institute of Molecular Plant Physiology
2020-2023
Friedrich Schiller University Jena
2023
Laboratoire de Biologie Moléculaire des Eucaryotes
2018
Université de Toulouse
2018
Université Toulouse III - Paul Sabatier
2018
Centre National de la Recherche Scientifique
2018
Summary The mTERF gene family encodes for nucleic acid binding proteins that are predicted to regulate organellar expression in eukaryotes. Despite the implication of this plant development and response abiotic stresses, a precise molecular function was assigned only handful number its c. 30 members plants. Using reverse genetics approach Arabidopsis thaliana combining biochemical techniques, we revealed new functions chloroplast protein, MDA1. We demonstrated MDA1 associates vivo with...
The early steps of the production large ribosomal subunit are probably least understood stages eukaryotic ribosome biogenesis. first specific precursor to yeast subunit, pre-60S particle, contains 30 assembly factors (AFs), including 8 RNA helicases. These helicases, presumed drive conformational rearrangements, usually lack substrate specificity in vitro. mechanisms by which they targeted their correct within pre-ribosomal particles and precise molecular roles remain largely unknown. We...
The mitochondrial transcription termination factor proteins are nuclear-encoded nucleic acid binders defined by degenerate tandem helical-repeats of ∼30 amino acids. They found in metazoans and plants where they localize organelles. In higher plants, the mTERF family comprises members several these have been linked to plant development response abiotic stress. However, knowledge molecular basis underlying physiological effects is scarce. We show that Arabidopsis mTERF9 protein promotes...
Abstract The conversion of light energy to chemical by photosynthesis requires the concerted action large protein complexes in thylakoid membrane. Recent work has provided fundamental insights into three-dimensional structure these complexes, but how they are assembled from hundreds parts remains poorly understood. Particularly little is known about biogenesis cytochrome b 6 f complex (Cyt ), redox-coupling that interconnects two photosystems. Here we report identification a factor guides...
Abstract Background Ribosome profiling (or Ribo-seq) is a technique that provides genome-wide information on the translational landscape (translatome). Across different plant studies, variable methodological setups have been described which raises questions about general comparability of data were generated from diverging methodologies. Furthermore, common problem when performing Ribo-seq are abundant rRNA fragments wastefully incorporated into libraries and dramatically reduce sequencing...
Abstract Ribosome profiling (or Ribo-seq) is a technique that provides genome-wide information on the translational landscape (translatome). Across different plant studies, variable methodological setups have been described which raises questions about general comparability of data were generated from diverging methodologies. Furthermore, common problem when performing Ribo-seq are abundant rRNA fragments wastefully incorporated into libraries and dramatically reduce sequencing depth. To...
In plant biotechnology and basic research, chloroplasts have been used as chassis for the expression of various transgenes. However, potential unintended side effects transgene insertion high-level on native chloroplast genes are often ignored not studied comprehensively. Here, we examined genome at both transcriptional translational levels in five transplastomic tobacco (Nicotiana tabacum) lines carrying identical aadA resistance marker cassette diverse genomic positions. Although none...
Abstract The pathway of photosystem II (PSII) assembly is well understood, and multiple auxiliary proteins supporting it have been identified, but little known about rate-limiting steps controlling PSII biogenesis. In the cyanobacterium Synechocystis PCC6803 green alga Chlamydomonas reinhardtii, indications exist that biosynthesis chloroplast-encoded D2 reaction center subunit (PsbD) limits accumulation. To determine importance synthesis for accumulation in vascular plants elucidate...
Abstract Gene regulation at the post-transcriptional level is prevalent in all domains of life. In bacteria, ProQ-like proteins have emerged as important RNA chaperones facilitating stability and duplex formation. major human pathogen Vibrio cholerae, gene key for virulence, biofilm formation, antibiotic resistance, yet role ProQ has not been studied. Here, we show that interacts with hundreds transcripts V. including highly abundant FlaX small (sRNA). Global analyses formation using RIL-Seq...
ABSTRACT The mitochondrial transcription termination factor proteins are nuclear-encoded nucleic acid binders defined by degenerate tandem helical-repeats of ~30 amino acids. They found in metazoans and plants where they localize to mitochondria or chloroplasts. In higher plants, the mTERF family comprises members several these have been linked plant development response abiotic stress. However, knowledge molecular basis underlying physiological effects is scarce. We show that Arabidopsis...
Abstract The pathway of photosystem II assembly is well understood and multiple auxiliary proteins supporting it have been identified. By contrast, little known about rate-limiting steps controlling PSII biogenesis. In the green alga Chlamydomonas reinhardtii , biosynthesis chloroplast-encoded D2 reaction center subunit (PsbD) limits accumulation. To determine importance synthesis for accumulation in vascular plants elucidate contributions transcriptional translational regulation,...