A5010816496- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Redox biology and oxidative stress
- Peptidase Inhibition and Analysis
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Sirtuins and Resveratrol in Medicine
- ATP Synthase and ATPases Research
- Histone Deacetylase Inhibitors Research
- Lipid metabolism and biosynthesis
- Antimicrobial Peptides and Activities
- Mass Spectrometry Techniques and Applications
- Click Chemistry and Applications
- Plant Pathogenic Bacteria Studies
- Seed Germination and Physiology
- Legume Nitrogen Fixing Symbiosis
- Advanced Proteomics Techniques and Applications
- Glutathione Transferases and Polymorphisms
- Selenium in Biological Systems
- Photoreceptor and optogenetics research
- Plant tissue culture and regeneration
Max Planck Institute for Plant Breeding Research
2014-2025
University of Münster
2016-2025
Helmholtz-Institute Münster
2024
Forschungszentrum Jülich
2024
Bielefeld University
2003-2024
Institute of Plant Biology and Biotechnology
2017-2022
Ludwig-Maximilians-Universität München
2012-2021
Technical University of Munich
2020
Urologische Klinik München
2013-2014
Max Planck Society
2014
Abstract Salicylic acid (SA) plays a key role in plant disease resistance and hypersensitive cell death but is also implicated hardening responses to abiotic stressors. Cadmium (Cd) exposure increased the free SA contents of barley (Hordeum vulgare) roots by factor about 2. Cultivation dry caryopses presoaked SA-containing solution for only 6 h or single transient addition at 0.5 mmconcentration hydroponics partially protected seedlings from Cd toxicity during following growth period. Both...
Abstract Nitric oxide (NO) is a free radical product of cell metabolism that plays diverse and important roles in the regulation cellular function. S-Nitrosylation emerging as specific fundamental posttranslational protein modification for transduction NO bioactivity, but very little known about its physiological functions plants. We investigated molecular mechanism S-nitrosylation peroxiredoxin II E (PrxII E) from Arabidopsis thaliana found this inhibits hydroperoxide-reducing peroxidase...
Abstract Acetylation of the ε-amino group lysine (Lys) is a reversible posttranslational modification recently discovered to be widespread, occurring on proteins outside nucleus, in most subcellular locations mammalian cells. Almost nothing known about this plants beyond well-studied acetylation histone nucleus. Here, we report that Lys also occurs organellar and cytosolic proteins. We identified 91 Lys-acetylated sites 74 diverse functional classes. Furthermore, our study suggests may an...
The time of seed germination is a major decision point in the life plants determining future growth and development. This timing controlled by dormancy, which prevents under favourable conditions. plant hormone abscisic acid (ABA) protein DELAY OF GERMINATION 1 (DOG1) are essential regulators dormancy. function ABA dormancy rather well understood, but role DOG1 still unknown. Here, we describe four phosphatases that interact with seeds. Two them belong to clade A type 2C phosphatases:...
Summary Mitochondria host vital cellular functions, including oxidative phosphorylation and co‐factor biosynthesis, which are reflected in their proteome. At the level plant mitochondria organized into hundreds of discrete functional entities, undergo dynamic fission fusion. It is individual organelle that operates living cell, yet biochemical physiological assessments have exclusively focused on characteristics large populations mitochondria. Here, we explore protein composition an average...
Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to detect pathogen effectors and activate local systemic defence. NRG1 ADR1 "helper" NLRs (RNLs) cooperate with enhanced disease susceptibility 1 (EDS1), senescence-associated gene 101 (SAG101) phytoalexin-deficient 4 (PAD4) lipase-like proteins mediate signalling from TIR domain NLR (TNLs). The mechanism of RNL/EDS1 family protein cooperation is not understood. Here, we present genetic molecular...
Abstract Peroxiredoxins (prxs) are peroxidases with broad substrate specificity. The seven prx genes expressed in Arabidopsis shoots were analyzed for their expressional response to changing photon fluence rates, oxidative stress, and ascorbate application. results reveal a highly variable gene-specific reducing oxidizing conditions. steady-state transcript amounts of the chloroplast-targeted prxs, namely two-cysteine (2-Cys) Q andprx II E, decreased upon application ascorbate.prx also...
Peroxiredoxins (Prx) have recently moved into the focus of plant and animal research in context development, adaptation, disease, as they function both antioxidant defense by reducing a broad range toxic peroxides redox signaling relating to adjustment cell metabolism. At-PrxII F is one six type II Prx identified genome Arabidopsis thaliana only that targeted mitochondrion. Therefore, it might be assumed functions similar human 2-Cys (PRDX3) (PRDX5) yeast 1-Cys likewise mitochondrial...
Glutaredoxins (Grxs) are small ubiquitous proteins of the thioredoxin (Trx) family, which catalyze dithiol–disulfide exchange reactions or reduce protein-mixed glutathione disulfides. In plants, several Trx-interacting have been isolated from different compartments, whereas very few Grx-interacting known. We describe here determination Grx target using a mutated poplar Grx, various tissular and subcellular plant extracts, liquid chromatography coupled to tandem mass spectrometry detection....
Superoxide dismutases (SODs) are key components of the plant antioxidant defense system. While plastidic and cytosolic isoforms have been extensively studied, importance mitochondrial SOD at a cellular whole-plant level has not established. To address this, transgenic Arabidopsis (Arabidopsis thaliana) plants were generated in which expression AtMSD1, encoding manganese (Mn)SOD, was suppressed by antisense. The strongest antisense line showed retarded root growth even under control...
Summary Peroxiredoxin Q (Prx Q) is one out of 10 peroxiredoxins encoded in the genome Arabidopsis thaliana , and four that are targeted to plastids. functions as a monomeric protein represents about 0.3% chloroplast proteins. It attaches thylakoid membrane detected preparations enriched photosystem II complexes. decomposes peroxides using thioredoxin an electron donor with substrate preference H 2 O > cumene hydroperoxide ≫ butyl linoleoyl insignificant affinity towards complex...
Histone deacetylases have central functions in regulating stress defenses and development plants. However, the knowledge about deacetylase is largely limited to histones, although these enzymes were found diverse subcellular compartments. In this study, we determined proteome-wide signatures of RPD3/HDA1 class histone Arabidopsis Relative quantification changes lysine acetylation levels was on a scale after treatment leaves with inhibitors apicidin trichostatin A. We identified 91 new...
Seeds preserve a far developed plant embryo in quiescent state. Seed metabolism relies on stored resources and is reactivated to drive germination when the external conditions are favorable. Since switchover from quiescence reactivation provides remarkable case of cell physiological transition we investigated earliest events energy redox Arabidopsis seeds at imbibition. By developing fluorescent protein biosensing intact seeds, observed ATP accumulation oxygen uptake within minutes,...
Abstract The posttranslational regulation of proteins by lysine (Lys) acetylation has recently emerged to occur not only on histones, but also organellar in plants and animals. In particular, the catalytic activities metabolic enzymes have been shown be regulated Lys acetylation. Arabidopsis (Arabidopsis thaliana) genome encodes two predicted sirtuin-type deacetylases, which Silent Information Regulator2 homolog (SRT2) contains a presequence for mitochondrial targeting. Here, we investigated...
Plant organelle function must constantly adjust to environmental conditions, which requires dynamic coordination. Ca(2+) signaling may play a central role in this process. Free dynamics are tightly regulated and differ markedly between the cytosol, plastid stroma, mitochondrial matrix. The mechanistic basis of compartment-specific is poorly understood. Here, we studied At-MICU, an EF-hand protein Arabidopsis thaliana with homology constituents uniporter machinery mammals. MICU binds...
Abstract Protein lysine acylations, such as succinylation and acetylation, are important post‐translational modification (PTM) mechanisms, with key roles in cellular regulation. Antibody‐based affinity enrichment, high‐resolution liquid chromatography mass spectrometry analysis, integrated bioinformatics analysis were used to characterize the succinylome (K suc ) acetylome ace of rice leaves. In total, 2,593 succinylated 1,024 acetylated proteins identified, which 723 simultaneously...
Mitochondria are defining components of most eukaryotes. However, higher plant mitochondria differ biochemically, morphologically, and dynamically from those in other FRIENDLY, a member the CLUSTERED MITOCHONDRIA superfamily, is conserved among eukaryotes required for correct distribution within cell. We sought to understand how disruption FRIENDLY function Arabidopsis (Arabidopsis thaliana) leads mitochondrial clustering effects this aberrant chondriome on cell whole-plant physiology....
Article7 July 2020Open Access Transparent process Dual lysine and N-terminal acetyltransferases reveal the complexity underpinning protein acetylation Willy V Bienvenut orcid.org/0000-0003-4192-3920 Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of Cell (I2BC), Gif-sur-Yvette, France Search more papers by this author Annika Brünje orcid.org/0000-0002-8979-4606 Plant Physiology, Biotechnology, University Muenster, Germany Jean-Baptiste Boyer...
Abstract Cysteine conjugation is an important tool in protein research and relies on fast, mild chemoselective reactions. Cysteinyl thiols can either be modified with prefunctionalized electrophiles, or converted into electrophiles themselves for functionalization selected nucleophiles independent step. Here we report a bioconjugation strategy that uses vinyl thianthrenium salt to transform cysteine highly reactive electrophilic episulfonium intermediate situ, enable diverse set of...