A5013356471- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Metal-Catalyzed Oxygenation Mechanisms
- Light effects on plants
- Redox biology and oxidative stress
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Plant responses to water stress
- Plant Micronutrient Interactions and Effects
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Metalloenzymes and iron-sulfur proteins
- Microbial bioremediation and biosurfactants
- Photoreceptor and optogenetics research
- Plant Pathogenic Bacteria Studies
- Glutathione Transferases and Polymorphisms
- Microbial Fuel Cells and Bioremediation
- Algal biology and biofuel production
- Plant-Microbe Interactions and Immunity
- Porphyrin Metabolism and Disorders
Central European Institute of Technology
2014-2023
Central European Institute of Technology – Masaryk University
2014-2023
Ghent University
2008-2015
VIB-UGent Center for Plant Systems Biology
2009-2015
National University of Rosario
1997-2009
Instituto de Biología Molecular y Celular de Rosario
2006-2009
Consejo Nacional de Investigaciones Científicas y Técnicas
2006-2009
Vlaams Instituut voor Biotechnologie
2008
Research Centre in Biological Chemistry of Córdoba
2007
Universidad Nacional de Córdoba
2007
Abstract Reactive oxygen species and redox signaling undergo synergistic antagonistic interactions with phytohormones to regulate protective responses of plants against biotic abiotic stresses. However, molecular insight into the nature this crosstalk remains scarce. We demonstrate that hydrogen peroxide–responsive UDP-glucosyltransferase UGT74E2 Arabidopsis thaliana is involved in modulation plant architecture water stress response through its activity toward auxin indole-3-butyric acid...
Attempted infection of plants by pathogens elicits a complex defensive response. In many non-host and incompatible host interactions it includes the induction defence-associated genes form localized cell death (LCD), purportedly designed to restrict pathogen advance, collectively known as hypersensitive response (HR). It is preceded an oxidative burst, generating reactive oxygen species (ROS) that are proposed cue subsequent deployment HR, although neither origin nor precise role played ROS...
Summary Peroxiredoxin Q (Prx Q) is one out of 10 peroxiredoxins encoded in the genome Arabidopsis thaliana , and four that are targeted to plastids. functions as a monomeric protein represents about 0.3% chloroplast proteins. It attaches thylakoid membrane detected preparations enriched photosystem II complexes. decomposes peroxides using thioredoxin an electron donor with substrate preference H 2 O > cumene hydroperoxide ≫ butyl linoleoyl insignificant affinity towards complex...
Abstract Chloroplast ferredoxin (Fd) plays a pivotal role in plant cell metabolism by delivering reducing equivalents to various essential oxidoreductive pathways. Fd levels decrease under adverse environmental conditions many microorganisms, including cyanobacteria, which share common ancestor with chloroplasts. Conversely, stress situations induce the synthesis of flavodoxin (Fld), an electron carrier flavoprotein not found plants, can efficiently replace most transfer processes. We report...
Abstract Arabidopsis (Arabidopsis thaliana) leaf development relies on subsequent phases of cell proliferation and expansion. During the phase, chloroplasts need to divide extensively, during transition from expansion, they differentiate into photosynthetically active chloroplasts, providing plant with energy. The transcription factor GROWTH REGULATING FACTOR5 (GRF5) promotes duration period development. Here, it is shown that GRF5 also stimulates chloroplast division, resulting in a higher...
Iron limitation affects one-third of the cultivable land on Earth and represents a major concern for agriculture. It causes decline many photosynthetic components, including Fe-S protein ferredoxin (Fd), involved in essential oxidoreductive pathways chloroplasts. In cyanobacteria some algae, Fd down-regulation under Fe deficit is compensated by induction an isofunctional electron carrier, flavodoxin (Fld), flavin mononucleotide-containing not found plants. Transgenic tobacco lines expressing...
Abstract Ferredoxin-NADP(H) reductase (FNR) catalyzes the last step of photosynthetic electron transport in chloroplasts, driving electrons from reduced ferredoxin to NADP+. This reaction is rate limiting for photosynthesis under a wide range illumination conditions, as revealed by analysis plants transformed with an antisense version FNR gene. To investigate whether accumulation this flavoprotein over wild-type levels could improve efficiency and growth, we generated transgenic tobacco...
Abiotic stress poses constant challenges for plant survival and is a serious problem global agricultural productivity. On molecular level, conditions result in elevation of reactive oxygen species (ROS) production causing oxidative associated with oxidation proteins nucleic acids as well impairment membrane functions. Adaptation root growth to ROS accumulation facilitated through modification auxin cytokinin hormone homeostasis. Here, we report that Arabidopsis meristem, ROS-induced changes...
Summary Ferredoxin‐NADP(H) reductase (FNR) catalyses the final step of photosynthetic electron transport in chloroplasts. Using an antisense RNA strategy to reduce expression this flavoenzyme transgenic tobacco plants, it has been demonstrated that FNR mediates a rate‐limiting photosynthesis under both limiting and saturating light conditions. Here, we show these FNR‐deficient plants are abnormally prone photo‐oxidative injury. When grown autotrophic conditions for 3 weeks, specimens with...
Leaf senescence is a concerted physiological process involving controlled degradation of cellular structures and reallocation breakdown products to other plant organs. It accompanied by increased production reactive oxygen species (ROS) that are proposed signal cell death, although both the origin precise role ROS in execution this developmental program still poorly understood. To investigate contribution chloroplast-associated natural leaf senescence, we used tobacco plants expressing...
Ferredoxin‐NADP + reductases (FNR) participate in cellular defense against oxidative damage. Escherichia coli mutants deficient FNR are abnormally sensitive to methyl viologen and hydrogen peroxide. Tolerance these oxidants was regained by expression of plant FNR, superoxide dismutase, or catalase genes the mutant cells. contribution concerted toxicity under redox‐cycling conditions similar that two major E. dismutases together, as judged phenotypes displayed relevant strains. However, sodA...
Photosynthesis is among the first processes negatively affected by environmental cues and its performance directly determines plant cell fitness ultimately crop yield. Primarily sites of photosynthesis, chloroplasts are unique also for biosynthesis precursors growth regulator auxin sensing stress, but their role in intracellular homeostasis, vital survival changing environments, remains poorly understood. Here, we identified two ATP-binding cassette (ABC) subfamily B transporters, ABCB28...
Significant effort has been directed in recent times to the use of plants extract and detoxify nitroaromatics from polluted industrial facilities. We have explored possibility overcoming phytotoxicity highly toxic recalcitrant nitroderivative 2,4-dinitrotoluene (2,4-DNT) by expressing a cyanobacterial flavodoxin (Fld) tobacco plants. demonstrate here that transformants accumulating Fld plastids display remarkable increase ability tolerate, take up, transform 2,4-DNT, as compared their...