A5002305226- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Metal-Catalyzed Oxygenation Mechanisms
- Algal biology and biofuel production
- Metalloenzymes and iron-sulfur proteins
- Hemoglobin structure and function
- Light effects on plants
- Plant Gene Expression Analysis
- Plant responses to water stress
- Photoreceptor and optogenetics research
- Plant-Microbe Interactions and Immunity
- Porphyrin Metabolism and Disorders
- Redox biology and oxidative stress
- Plant responses to elevated CO2
- Enzyme Structure and Function
- Microbial Fuel Cells and Bioremediation
- Plant nutrient uptake and metabolism
- Enzyme Catalysis and Immobilization
- Electrochemical sensors and biosensors
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Mass Spectrometry Techniques and Applications
- Plant Reproductive Biology
- Microbial bioremediation and biosurfactants
National University of Rosario
2015-2024
Instituto de Biología Molecular y Celular de Rosario
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2024
Hospital General San Juan de Dios
2016
Universidad Nacional de Córdoba
2007
Research Centre in Biological Chemistry of Córdoba
2007
Leibniz Institute of Plant Genetics and Crop Plant Research
2006
Universidad de Zaragoza
2004-2005
Instituto de Química Física Blas Cabrera
2005
University College Dublin
2004
The antioxidant defense system involves complex functional coordination of multiple components in different organelles within the plant cell. Here, we have studied Arabidopsis thaliana early response to generation superoxide anion chloroplasts during active photosynthesis. We exposed plants methyl viologen (MV), a propagator light, and performed biochemical expression profiling experiments using Affymetrix ATH1 GeneChip® microarrays under conditions which photosynthesis enzymes were active....
Attempted infection of plants by pathogens elicits a complex defensive response. In many non-host and incompatible host interactions it includes the induction defence-associated genes form localized cell death (LCD), purportedly designed to restrict pathogen advance, collectively known as hypersensitive response (HR). It is preceded an oxidative burst, generating reactive oxygen species (ROS) that are proposed cue subsequent deployment HR, although neither origin nor precise role played ROS...
Abstract Chloroplast ferredoxin (Fd) plays a pivotal role in plant cell metabolism by delivering reducing equivalents to various essential oxidoreductive pathways. Fd levels decrease under adverse environmental conditions many microorganisms, including cyanobacteria, which share common ancestor with chloroplasts. Conversely, stress situations induce the synthesis of flavodoxin (Fld), an electron carrier flavoprotein not found plants, can efficiently replace most transfer processes. We report...
Summary Reactive oxygen species ( ROS ) play fundamental roles in plant responses to pathogen infection, including modulation of cell death processes and defense‐related gene expression. Cell triggered as part the hypersensitive response enhances resistance biotrophic pathogens, but favors virulence necrotrophs. Even though involvement orchestration defense is well established, relative contribution specific subcellular sources against microorganisms with different pathogenesis strategies...
The ability of plants to maintain photosynthesis in a dynamically changing environment is central importance for their growth. As the photosynthetic machinery sensitive and early target adverse environmental conditions as those typically found field, efficiency not always optimal. Cyanobacteria, algae, mosses, liverworts gymnosperms produce flavodiiron proteins (Flvs), class electron sinks represented angiosperms; these act mitigate photoinhibition photosystem I under high or fluctuating...
Iron limitation affects one-third of the cultivable land on Earth and represents a major concern for agriculture. It causes decline many photosynthetic components, including Fe-S protein ferredoxin (Fd), involved in essential oxidoreductive pathways chloroplasts. In cyanobacteria some algae, Fd down-regulation under Fe deficit is compensated by induction an isofunctional electron carrier, flavodoxin (Fld), flavin mononucleotide-containing not found plants. Transgenic tobacco lines expressing...
Summary Nitric oxide (NO) is a signaling molecule with diverse biological functions in plants. NO plays crucial role growth and development, from germination to senescence, also involved plant responses biotic abiotic stresses. In animals, synthesized by well‐described nitric synthase (NOS) enzymes. NOS activity has been detected higher plants, but no gene encoding an protein, or the enzymes required for synthesis of tetrahydrobiopterin, essential cofactor mammalian activity, have identified...
NADP(H) is a central metabolic hub providing reducing equivalents to multiple biosynthetic, regulatory and antioxidative pathways in all living organisms. While biosensors are available determine NADP+ or NADPH levels vivo, no probe exists estimate the redox status, determinant of cell energy availability. We describe herein design characterization genetically-encoded ratiometric biosensor, termed NERNST, able interact with ENADP(H). NERNST consists redox-sensitive green fluorescent protein...
Summary A ferredoxin‐NADP + oxidoreductase (FNR) cDNA from tobacco ( Nicotiana tabacum cv. Samsun) was cloned and sequenced. Comparison of the deduced amino acid sequence revealed high identity to FNR proteins Capsicum annuum, Pisum sativum, Spinacia oleracea Vicia faba. Transgenic plants were generated that constitutively express in reverse orientation between CaMV 35S promoter polyadenylation signal octopine synthase gene. Plants expressing antisense gene showed lower levels mRNA protein...
Abstract Ferredoxin-NADP(H) reductase (FNR) catalyzes the last step of photosynthetic electron transport in chloroplasts, driving electrons from reduced ferredoxin to NADP+. This reaction is rate limiting for photosynthesis under a wide range illumination conditions, as revealed by analysis plants transformed with an antisense version FNR gene. To investigate whether accumulation this flavoprotein over wild-type levels could improve efficiency and growth, we generated transgenic tobacco...
The soxRS regulon protects Escherichia coli cells against superoxide and nitric oxide. Oxidation of the SoxR sensor, a [2Fe-2S]-containing transcriptional regulator, triggers response, but nature cellular signal sensed by is still matter debate. In vivo, sensor maintained in reduced, inactive state activities reductases, which employ NADPH as an electron donor. hypothesis that levels affect deployment response was tested transforming E. with genes encoding enzymes proteins lead to either...
Plants constitute a source of novel phytotoxic compounds to be explored in searching for effective and environmentally safe herbicides. From previous screening plant extracts their phytotoxicity, dichloromethane extract Ammi visnaga (L.) Lam. was selected further study. Phytotoxicity-guided fractionation this yielded two furanochromones, khellin visnagin, which herbicidal activity had not been described before. Khellin visnagin were model species lettuce (Lactuca sativa) duckweed (Lemna...
Light significantly influences plant stress responses, with chloroplasts playing a pivotal role as both energy providers and light sensors. They communicate the nucleus through retrograde signals, including secondary metabolites reactive oxygen species (ROS). To investigate contribution of chloroplast redox biochemistry to biotic we studied interactions tobacco leaves expressing alternative electron shuttle flavodoxin virulent nonhost Pseudomonas syringae pathovars under dark conditions....
Chloroplast ferredoxin-NADP<sup>+</sup>reductase has a 32,000-fold preference for NADPH over NADH, consistent with its main physiological role of NADP<sup>+</sup> photoreduction <i>de novo</i> carbohydrate biosynthesis. Although it is distant from the 2′-phosphoryl group NADP<sup>+</sup>, replacement C-terminal tyrosine (Tyr<sup>308</sup> in pea enzyme) by Trp, Phe, Gly, and Ser produced enzyme forms which NADH was decreased about 2-, 10-, 300-, 400-fold, respectively. Remarkably, case Y308S...