A5038767441- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Algal biology and biofuel production
- Nitric Oxide and Endothelin Effects
- Plant responses to water stress
- Marine and coastal ecosystems
- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
- Plant responses to elevated CO2
- Light effects on plants
- Insect and Arachnid Ecology and Behavior
- Electron Spin Resonance Studies
- Plant Water Relations and Carbon Dynamics
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Ocean Acidification Effects and Responses
- Hemoglobin structure and function
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Paleontology and Stratigraphy of Fossils
- Redox biology and oxidative stress
- Polysaccharides and Plant Cell Walls
Consejo Nacional de Investigaciones Científicas y Técnicas
2013-2024
National University of Mar del Plata
2015-2024
Fundación Ciencias Exactas y Naturales
2008-2022
University of Saskatchewan
2008
University of Guelph
2008
University of Alberta
2008
Abstract The search for a nitric oxide synthase (NOS) sequence in the plant kingdom yielded two sequences from recently published genomes of green algae species Ostreococcus genus, O. tauri and lucimarinus. In this study, we characterized sequence, protein structure, phylogeny, biochemistry, expression NOS tauri. amino acid was found to be 45% similar that human NOS. Folding assignment methods showed can fold as endothelial isoform. Phylogenetic analysis revealed clusters together with...
Nitric oxide (NO) is a bioactive molecule involved in diverse physiological functions plants. It has previously been reported that the NO donor sodium nitroprusside (SNP) applied to germinated tomato seeds was able induce lateral root (LR) formation same way auxin treatment does. In this paper, it shown modulates expression of cell cycle regulatory genes pericycle cells and leads, turn, induced LR formation. The addition scavenger CPTIO at different time points during auxin-mediated...
Abstract Mutation of either arginase structural gene (ARGAH1 or ARGAH2 encoding arginine [Arg] amidohydrolase-1 and -2, respectively) resulted in increased formation lateral adventitious roots Arabidopsis (Arabidopsis thaliana) seedlings nitric oxide (NO) accumulation efflux, detected by the fluorogenic traps 3-amino,4-aminomethyl-2′,7′-difluorofluorescein diacetate diamino-rhodamine-4M, respectively. Upon seedling exposure to synthetic auxin naphthaleneacetic acid, NO was differentially...
S-Nitrosylation of Cys residues is one the molecular mechanisms driven by nitric oxide (NO) for regulating biological functions key proteins. While studies on S-nitrosylation have served identifying SNO proteomes, physiological relevance protein S-nitrosylation/denitrosylation remains poorly understood. In this study, it shown that auxin influences balance S-nitrosylated/denitrosylated proteins in roots Arabidopsis seedlings. 2D-PAGE allowed identification ascorbate peroxidase 1 (APX1) as...
Summary Nitric oxide (NO) is a signaling molecule with diverse biological functions in plants. NO plays crucial role growth and development, from germination to senescence, also involved plant responses biotic abiotic stresses. In animals, synthesized by well‐described nitric synthase (NOS) enzymes. NOS activity has been detected higher plants, but no gene encoding an protein, or the enzymes required for synthesis of tetrahydrobiopterin, essential cofactor mammalian activity, have identified...
Nitric oxide (NO) is a bioactive molecule involved in several growth and developmental processes plants. These are mostly characterized by changes primary secondary metabolism. Here, the effect of NO on cellulose synthesis tomato (Solanum lycopersicum) roots was studied. The phenotype roots, content, incorporation 14C-glucose into cellulosic fraction expression synthase (CESA) transcripts treated with donor sodium nitroprusside (SNP) were analysed. affected content dose dependent manner. Low...
The F-box proteins (FBPs) TIR1/AFBs are the substrate recognition subunits of SKP1–cullin–F-box (SCF) ubiquitin ligase complexes and together with Aux/IAAs form auxin co-receptor. Although tremendous knowledge on perception signaling has been gained in last years, SCFTIR1/AFBs complex assembly stabilization emerging as new layers regulation. Here, we investigated how nitric oxide (NO), through S-nitrosylation ASK1 is involved assembly. We demonstrate that S-nitrosylated S-glutathionylated...
Auxin is the main phytohormone controlling root development in plants. This study uses pharmacological and genetic approaches to examine role of auxin nitric oxide (NO) activation NADPH-dependent thioredoxin reductase (NTR), effect that this activity has on growth responses Arabidopsis thaliana.Arabidopsis seedlings were treated with or without NTR inhibitors auranofin (ANF) 1-chloro-2, 4-dinitrobenzene (DNCB). activity, lateral (LR) formation S-nitrosothiol content measured roots. Protein...
OPINION article Front. Plant Sci., 04 July 2013Sec. Physiology https://doi.org/10.3389/fpls.2013.00232
Abstract The enzyme nitric oxide synthase (NOS) oxidizes L-arginine to NO and citrulline. In this work, we characterise the NOS from cyanobacteria Synechococcus PCC 7335 (SyNOS). SyNOS possesses a canonical mammalian architecture consisting of oxygenase reductase domains. addition, an unusual globin domain at N-terminus. Recombinant expressed in bacteria is active, its activity suppressed by inhibitor L-NAME. allows E. coli grow minimum media containing as sole N source, has higher growth...
Introduction Photosynthetic organisms respond to nitrogen (N) deprivation with the slowdown of photosynthesis and electron transport resulting in balance carbon (C)/N ratio. Under this extreme condition, trigger complex mechanisms keep growing using different N sources recycling containing molecules. In particular, phytoplankton are able uptake L-arginine (L-Arg) as an organic source. L-Arg can be assimilated mainly by arginase, arginine deimidase, decarboxylase or L-amino oxidase pathways....
Aims: Nitrogen (N) is a necessary nutrient for plant development and seed production, with nitrate (NO3-) serving as the primary source of N in soils. Although several molecular players responses to NO3- signaling were unraveled, it still complex process gaps that require further investigation. The aim our study analyze role nitric oxide (NO) response (PNR). Results: Using combination genetic pharmacological approaches, we demonstrate NO required expression NO3--regulated genes reductase 1...
Nitric oxide (NO) is a messenger molecule widespread studied in plant physiology. Latter evidence supports the lack of NO-producing system involving NO synthase (NOS) activity higher plants. However, NOS gene from unicellular marine alga Ostreococcus tauri (OtNOS) was characterized recent years. OtNOS genuine NOS, with similar spectroscopic fingerprints to mammalian NOSs and high producing capacity. We are interested investigating whether expression can improve growth production tobacco....
Fil: Foresi, Noelia Pamela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto Biologicas. Universidad Facultad Ciencias Exactas Naturales. Biologicas; Argentina