A5079701047- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- GABA and Rice Research
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Trypanosoma species research and implications
- Plant Taxonomy and Phylogenetics
- Algal biology and biofuel production
- Antioxidant Activity and Oxidative Stress
- Heat shock proteins research
- Metal-Catalyzed Oxygenation Mechanisms
- Biochemical Analysis and Sensing Techniques
- Potato Plant Research
- Advanced Chemical Sensor Technologies
- Legume Nitrogen Fixing Symbiosis
- Mitochondrial Function and Pathology
- Plant Micronutrient Interactions and Effects
- Plant Genetic and Mutation Studies
- Metalloenzymes and iron-sulfur proteins
- Plant tissue culture and regeneration
Consejo Nacional de Investigaciones Científicas y Técnicas
2011-2021
Instituto de Biología Molecular y Celular de Rosario
2011-2021
National University of Rosario
2008-2021
Lethbridge Research and Development Centre
2004-2015
Agriculture and Agri-Food Canada
2004-2015
Instituto de Ciências Farmacêuticas
2004-2012
Institute of Scientific and Technical Research for Defense
2011
University of Cologne
2008
Universidad Nacional de Córdoba
2007
Research Centre in Biological Chemistry of Córdoba
2007
The antioxidant defense system involves complex functional coordination of multiple components in different organelles within the plant cell. Here, we have studied Arabidopsis thaliana early response to generation superoxide anion chloroplasts during active photosynthesis. We exposed plants methyl viologen (MV), a propagator light, and performed biochemical expression profiling experiments using Affymetrix ATH1 GeneChip® microarrays under conditions which photosynthesis enzymes were active....
Abstract Chloroplast ferredoxin (Fd) plays a pivotal role in plant cell metabolism by delivering reducing equivalents to various essential oxidoreductive pathways. Fd levels decrease under adverse environmental conditions many microorganisms, including cyanobacteria, which share common ancestor with chloroplasts. Conversely, stress situations induce the synthesis of flavodoxin (Fld), an electron carrier flavoprotein not found plants, can efficiently replace most transfer processes. We report...
Abstract Arabidopsis (Arabidopsis thaliana) overexpressing glycolate oxidase (GO) in chloroplasts accumulates both hydrogen peroxide (H2O2) and glyoxylate. GO-overexpressing lines (GO plants) grown at 75 μmol quanta m−2 s−1 show retarded development, yellowish rosettes, impaired photosynthetic performance, while 30 s−1, this phenotype virtually disappears. The GO plants develop oxidative stress lesions under photorespiratory conditions but grow like wild-type nonphotorespiratory conditions....
Iron limitation affects one-third of the cultivable land on Earth and represents a major concern for agriculture. It causes decline many photosynthetic components, including Fe-S protein ferredoxin (Fd), involved in essential oxidoreductive pathways chloroplasts. In cyanobacteria some algae, Fd down-regulation under Fe deficit is compensated by induction an isofunctional electron carrier, flavodoxin (Fld), flavin mononucleotide-containing not found plants. Transgenic tobacco lines expressing...
Oxidative stress, arising from an imbalance in the generation and removal of reactive oxygen species (ROS), is a challenge faced by all aerobic organisms. In plants, different pathways sense ROS extracellular sources or organelles such as mitochondria, chloroplast peroxisome. our recent paper on Plant Molecular Biology1 we have studied Arabidopsis thaliana early response to superoxide anion chloroplasts during active photosynthesis. Transcript profile analysis revealed that expression level...
The transcription factor superfamily, APETALA2/ethylene response factor, is involved in plant growth and development, as well environmental stress responses. Here, an uncharacterized gene of this family, AtERF019, was studied Arabidopsis thaliana under abiotic situations. plants overexpressing AtERF019 showed a delay flowering time 7 days senescence 2 weeks when comparison with wild type plants. These also increased tolerance to water deficiency that could be explained by lower transpiration...
Summary A ferredoxin‐NADP + oxidoreductase (FNR) cDNA from tobacco ( Nicotiana tabacum cv. Samsun) was cloned and sequenced. Comparison of the deduced amino acid sequence revealed high identity to FNR proteins Capsicum annuum, Pisum sativum, Spinacia oleracea Vicia faba. Transgenic plants were generated that constitutively express in reverse orientation between CaMV 35S promoter polyadenylation signal octopine synthase gene. Plants expressing antisense gene showed lower levels mRNA protein...
Abstract Ferredoxin-NADP(H) reductase (FNR) catalyzes the last step of photosynthetic electron transport in chloroplasts, driving electrons from reduced ferredoxin to NADP+. This reaction is rate limiting for photosynthesis under a wide range illumination conditions, as revealed by analysis plants transformed with an antisense version FNR gene. To investigate whether accumulation this flavoprotein over wild-type levels could improve efficiency and growth, we generated transgenic tobacco...
Oxidative stress is involved in the response of Lycopersicon esculentum fruits (cultivar Micro‐Tom) to chilling. Changes activated oxygen scavenging enzymes, superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, 1.11.1.6), ascorbate peroxidase (APX, 1.11.1.11), and glutathione reductase (GR, 1.6.4.2) were examined during ripening after postharvest Also, lipid peroxidation, respiration, pigment contents determined. These parameters affected by chilling, especially lycopene content...
Globally, methane (CH4) emissions account for 40% to 45% of greenhouse gas from ruminant livestock, with over 90% these arising enteric fermentation. Reduction carbon dioxide CH4 is critical efficient ruminal fermentation because it prevents the accumulation reducing equivalents in rumen. Methanogens exist a symbiotic relationship rumen protozoa and fungi within biofilms associated feed wall. Genomics transcriptomics are playing an increasingly important role defining ecology methanogenesis...
The stability of chloroplastic glutamine synthetase (GS; EC 6.3.1.2) was investigated under photooxidative stress using wheat (Triticum aestivum L.) leaves, chloroplasts, and chloroplast lysates. Illuminated seedlings sprayed with the superoxide radical (O-(2)) propagator methyl viologen showed rapid GS decline dependent on MV concentration exposure time. Degradation products approximately 39 31 kD were detected when lysates containing both stroma thylakoids illuminated in presence or...
Abstract The flavoenzyme ferredoxin-NADP+ reductase (FNR) is a member of the cellular defense barrier against oxidative damage in Escherichia coli. We evaluated responses chloroplast FNR to methyl viologen, superoxide radical propagator, wheat (Triticum aestivum L.) plants and chloroplasts. Treatments with herbicide showed little effect on levels protein or transcripts, indicating that expression this not up-regulated by oxidants plants. Viologens peroxides caused solubilization active from...
Plants have the largest number of small heat shock proteins (sHsps) (15-42 kDa) among eukaryotes, but little is known about their function in vivo. They accumulate response to different stresses, and specific sHsps are also expressed during developmental processes such as seed development, germination, ripening. The presence organelle-specific appears be unique plants. expression regulated by stress transcription factors (Hsfs). In this work, it was explored role chilling injury tomato...
Pure phloem sap of tomato leaves was collected by stylectomy. Glutamine and glutamate were the predominant free amino acids translocated stream. In developing fruits glutamine content increased significantly, reaching 35% total acids. Comparison in acid composition between two tissues are discussed.
Summary Ferredoxin‐NADP(H) reductase (FNR) catalyses the final step of photosynthetic electron transport in chloroplasts. Using an antisense RNA strategy to reduce expression this flavoenzyme transgenic tobacco plants, it has been demonstrated that FNR mediates a rate‐limiting photosynthesis under both limiting and saturating light conditions. Here, we show these FNR‐deficient plants are abnormally prone photo‐oxidative injury. When grown autotrophic conditions for 3 weeks, specimens with...
Summary X anthomonas axonopodis pv. citri ( ac), the bacterium that causes citrus canker, contains a gene in hrp [for hypersensitive response (HR) and pathogenicity] cluster encodes harpin protein called H pa1. pa1 produced HR nonhost plants tobacco, pepper A rabidopsis, whereas, host plant citrus, it elicited weak defence with no visible phenotype. Co‐infiltrations of ac or without recombinant leaves larger number cankers presence protein. To characterize effect during disease, an acΔhpa1...