A5013004164- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Redox biology and oxidative stress
- Heat shock proteins research
- Sirtuins and Resveratrol in Medicine
- Autophagy in Disease and Therapy
- Plant Micronutrient Interactions and Effects
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Lipid Membrane Structure and Behavior
- Glutathione Transferases and Polymorphisms
- Signaling Pathways in Disease
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Light effects on plants
- Nanopore and Nanochannel Transport Studies
- Genomics, phytochemicals, and oxidative stress
- Ubiquitin and proteasome pathways
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Genomics and Chromatin Dynamics
- Plant responses to water stress
- ATP Synthase and ATPases Research
- Nitric Oxide and Endothelin Effects
Bielefeld University
2006-2019
Leibniz University Hannover
2013-2019
Ludwig-Maximilians-Universität München
2014
University of Oxford
2008-2011
Abstract Nitric oxide (NO) is a free radical product of cell metabolism that plays diverse and important roles in the regulation cellular function. S-Nitrosylation emerging as specific fundamental posttranslational protein modification for transduction NO bioactivity, but very little known about its physiological functions plants. We investigated molecular mechanism S-nitrosylation peroxiredoxin II E (PrxII E) from Arabidopsis thaliana found this inhibits hydroperoxide-reducing peroxidase...
Abstract Acetylation of the ε-amino group lysine (Lys) is a reversible posttranslational modification recently discovered to be widespread, occurring on proteins outside nucleus, in most subcellular locations mammalian cells. Almost nothing known about this plants beyond well-studied acetylation histone nucleus. Here, we report that Lys also occurs organellar and cytosolic proteins. We identified 91 Lys-acetylated sites 74 diverse functional classes. Furthermore, our study suggests may an...
Summary Peroxiredoxin Q (Prx Q) is one out of 10 peroxiredoxins encoded in the genome Arabidopsis thaliana , and four that are targeted to plastids. functions as a monomeric protein represents about 0.3% chloroplast proteins. It attaches thylakoid membrane detected preparations enriched photosystem II complexes. decomposes peroxides using thioredoxin an electron donor with substrate preference H 2 O > cumene hydroperoxide ≫ butyl linoleoyl insignificant affinity towards complex...
Abstract The posttranslational regulation of proteins by lysine (Lys) acetylation has recently emerged to occur not only on histones, but also organellar in plants and animals. In particular, the catalytic activities metabolic enzymes have been shown be regulated Lys acetylation. Arabidopsis (Arabidopsis thaliana) genome encodes two predicted sirtuin-type deacetylases, which Silent Information Regulator2 homolog (SRT2) contains a presequence for mitochondrial targeting. Here, we investigated...
DOI: 10.1111/j.1469-8137.2009.02731.x Commentary p 505
Cyps (cyclophilins) are ubiquitous proteins of the immunophilin superfamily with proposed functions in protein folding, degradation, stress response and signal transduction. Conserved cysteine residues further suggest a role redox regulation. In order to get insight into conformational change mechanism functional properties chloroplast-located CYP20-3, site-directed mutagenized cysteine→serine variants were generated analysed for enzymatic under reducing oxidizing conditions. Compared...
To cope with the various environmental stresses resulting in reactive oxygen species (ROS) production plant metabolism is known to be altered specifically under different stresses. After overcoming stress should reconfigured recover basal operation however knowledge concerning how this achieved cursory. investigate metabolic recovery of roots following oxidative stress, changes metabolite abundance and carbon flow were analysed. Arabidopsis treated by menadione elicit stress. Roots fed (13)C...
Abstract Photorespiration is essential for the detoxification of glycolate and recycling carbon to Calvin Benson Bassham cycle. Enzymes participating in pathway have been identified, investigations now focus on regulation photorespiration by transporters metabolites. However, gene level has not intensively studied. Here, we show that maximum transcript abundance Glu:glyoxylate aminotransferase 1 (GGT1) regulated intron-mediated enhancement (IME) 5′ leader intron rather than regulatory...
We investigated the threading and controlled translocation of individual lambda-DNA (λ-DNA) molecules through solid-state nanopores with piconewton force sensitivity, millisecond time resolution picoampere ionic current sensitivity a set-up combining quantitative 3D optical tweezers (OT) electrophysiology. With our virtually interference-free OT binding RecA single peroxiredoxin protein to λ-DNA was quantitatively during dynamic experiments where effective forces respective currents threaded...
The transition of C3 , via C2 towards C4 photosynthesis is an important example stepwise evolution a complex genetic trait. A common feature that was gradually emphasized during this trajectory the CO2 concentration mechanism around Rubisco. In plants, based on tissue-specific accumulation glycine decarboxylase (GDC) in bundle sheath (BS) cells, relative to global expression cells leaves. This limits photorespiratory release BS cells. Because are surrounded by photosynthetically active...
The non-proteinogenic amino acid ornithine links several stress response pathways. From a previous study we know that accumulates in to low CO2. To investigate accumulation plants, shifted plants either CO2 or light. Both conditions increased carbon limitation, but only also the rate of photorespiration. Changes metabolite profiles light- and CO2-limited were quite similar. Several acids are known markers senescence accumulated strongly under both conditions. However, urea cycle...
The genome of Synechococcus elongatus PCC 7942 encodes six peroxiredoxins (Prx). Single genes are present each for a 1-Cys Prx and 2-Cys Prx, while four code PrxQ-like proteins (prxQ-A1, -A2, -A3 B). Their transcript accumulation varies with growth conditions in gene-specific manner (Stork et al. J Exp Bot 56:3193-3206, 2005). To address their functional properties, members the prx gene family were produced as recombinant analysed peroxide detoxification capacity quaternary structure by size...
Being a pathway tightly linked to photosynthesis, photorespiration is regulated by light, but also both nutrients and metabolites on transcriptional level. However, only little known about the signals how they are integrated promoter level coordinate whole pathway. Using bioinformatic approach we analyzed co-expression patterns of photorespiratory genes, predicted cis-regulatory elements in their 5′ upstream regions existence introns 5′UTRs. We found that there groups genes strongly...