- GABA and Rice Research
- Phytochemicals and Antioxidant Activities
- Plant tissue culture and regeneration
- Cannabis and Cannabinoid Research
- Plant biochemistry and biosynthesis
- Polyamine Metabolism and Applications
- Cancer therapeutics and mechanisms
- Plant Gene Expression Analysis
- Food Quality and Safety Studies
- Phytoestrogen effects and research
- Tea Polyphenols and Effects
- Potato Plant Research
- Plant-Microbe Interactions and Immunity
- Postharvest Quality and Shelf Life Management
- Antioxidant Activity and Oxidative Stress
- Metabolomics and Mass Spectrometry Studies
- Plant Physiology and Cultivation Studies
- Biochemical Analysis and Sensing Techniques
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Nitrogen and Sulfur Effects on Brassica
- Coffee research and impacts
- Genomics, phytochemicals, and oxidative stress
- Microbial Natural Products and Biosynthesis
- Antimicrobial Peptides and Activities
Chulalongkorn University
2016-2025
Srinakharinwirot University
2018
National Center for Genetic Engineering and Biotechnology
2018
Chiba University
2007-2015
University of Copenhagen
2008-2009
Kyushu University
2003-2007
Delta(1)-tetrahydrocannabinolic acid (THCA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic into THCA, precursor Delta(1)-tetrahydrocannabinol. We cloned a novel cDNA (GenBank trade mark accession number AB057805) encoding THCA by reverse transcription and polymerase chain reactions from rapidly expanding leaves Cannabis sativa. This gene consists 1635-nucleotide open reading frame, 545-amino polypeptide which first 28 amino residues constitute signal peptide....
Tetrahydrocannabinolic acid (THCA) synthase is the enzyme responsible for production of tetrahydrocannabinol (THC), psychoactive component marijuana (Cannabis sativa L.). We suggest herein that THCA biosynthesized in storage cavity glandular trichomes based on following observations. (i) The exclusive expression was confirmed secretory cells by reverse transcription-PCR (RT-PCR) analysis. (ii) activity detected content. (iii) Transgenic tobacco expressing fused to green fluorescent protein...
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, dominant cannabinoid constituent fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA by reverse transcription and polymerase chain reactions with degenerate gene-specific primers. Biochemical characterization recombinant demonstrated covalently flavinylated oxidase. The structural functional properties are quite similar to those tetrahydrocannabinolic-acid...
Durian (Durio zibethinus L.), a tropical fruit celebrated in Southeast Asia for its distinct flavor, is the focus of this comprehensive review. The fruit’s pulp rich high-value bioactive compounds, including gamma-glutamylcysteine, precursor to essential antioxidant glutathione. With durian cultivation gaining prominence due economic potential through cultivar enhancement, an in-depth examination durian-related research becomes crucial. This review explores health benefits durian, analyzing...
Plants produce a variety of toxic compounds, which are often used as anticancer drugs. The self-resistance mechanism to these metabolites in the producing plants, however, remains unclear. plant-derived alkaloid camptothecin (CPT) induces cell death by targeting DNA topoisomerase I (Top1), enzyme that catalyzes changes topology. We found CPT-producing including Camptotheca acuminata, Ophiorrhiza pumila, and liukiuensis, have Top1s with point mutations confer resistance CPT, suggesting effect...
Isocitrate lyase (ICL) is a key enzyme in the glyoxylate cycle. In previous study rice, expression of ICL-encoding gene (OsICL) was highly induced by salt stress and its enhanced transgenic rice lines overexpressing OsCam1-1, calmodulin (CaM)-encoding gene. CaM has been implicated tolerance mechanisms plants; however, cellular mediated are not clearly understood. this study, role OsICL plant possible involvement were investigated using plants expressing or OsCam1-1.OsICL expressed senesced...
DNA binding with one finger (Dof) proteins constitute a ubiquitous plant-specific transcription factor (TF) family associated diverse biological processes, including ripening. We conducted genome-wide analysis of durian (Durio zibethinus Murr.) and identified 24 Dofs (DzDofs), 15 which were expressed in fruit pulp. Gene expression revealed differential DzDofs during ripening two commercial cultivars from Thailand, Monthong Chanee. Comparing the levels pulp-expressed between ten potential...
Fruit ripening is a highly coordinated developmental process driven by complex hormonal network. Ethylene the main regulator of climacteric fruit ripening. However, putative role other key phytohormones in this cannot be excluded. We previously observed an increasing level auxin during post-harvest durian fruit, which occurred concomitantly with rise ethylene biosynthesis. Herein, we connect signaling component, response factors (ARFs), regulatory network that controls through identification...
Sunflower (Helianthus annuus L.) sprouts accumulate high amounts of caffeoylquinic acids (CQAs) including chlorogenic acid (5-CQA) and 1,5-diCQA. These compounds, which can be found in many plants, tomato, globe artichoke, chicory, have health benefits, antioxidant, antihepatotoxic, antiglycative activities. However, CQA profiles biosynthesis not previously been studied sunflower sprouts. In the present study, we that 5-CQA 1,5-diCQA were major CQAs We also identified minor accumulation...
Durian is an economically important fruit of Southeast Asia. There is, however, a lack in-depth information on the alteration its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from pulp durian cv. Monthong at unripe and ripe stages, using capillary electrophoresis- gas chromatography- time-of-flight mass spectrometry, specifically focusing taste-related metabolites. During ripening, sucrose content increased. Change in raffinose-family...
Summary Studies have recently highlighted potential health benefits of young rice leaves; however, optimization extraction process was often overlooked. The present study aimed to assess impacts methods, namely, maceration and decoction, on total phenolics content antioxidant activities based FRAP, DPPH, ABTS assays extracts from Thai cultivar Jasmine leaves. examined influences ethanol concentration (0–100% (v/v) for maceration), boiling time (10–30 min decoction) solvent‐to‐solid ratio...