A5064754063- Plant nutrient uptake and metabolism
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Plant Physiology and Cultivation Studies
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant Micronutrient Interactions and Effects
- Plant Gene Expression Analysis
- Soil Carbon and Nitrogen Dynamics
- Algal biology and biofuel production
- GABA and Rice Research
- Plant biochemistry and biosynthesis
- Postharvest Quality and Shelf Life Management
- Plant Reproductive Biology
- Polyamine Metabolism and Applications
- Weed Control and Herbicide Applications
- Nitrogen and Sulfur Effects on Brassica
- CRISPR and Genetic Engineering
- Microbial Community Ecology and Physiology
- Allelopathy and phytotoxic interactions
- Rice Cultivation and Yield Improvement
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
Yamagata University
2015-2025
Kyoto University
1999-2025
RIKEN Center for Sustainable Resource Science
2014-2024
Yamagata University Hospital
2021
Osaka Prefecture University
2006-2014
Plant (United States)
2011-2013
RIKEN
2011
Chiba University
2007-2011
Institute of Agrobiological Sciences
2011
University of Science and Technology of China
2008
Abstract Despite its relevance for agricultural production, environmental stress-induced growth inhibition, which is responsible significant yield reductions, only poorly understood. Here, we investigated the molecular mechanisms underlying cell cycle inhibition in young proliferating leaves of model plant Arabidopsis thaliana when subjected to mild osmotic stress. A detailed cellular analysis demonstrated that as soon stress sensed, progression rapidly arrests, but cells are kept a latent...
Summary A comprehensive and large‐scale metabolome quantitative trait loci (mQTL) analysis was performed to investigate the genetic backgrounds associated with metabolic phenotypes in rice grains. The dataset consisted of 759 metabolite signals obtained from grains 85 lines ( Oryza sativa , Sasanishiki × Habataki back‐crossed inbred lines). Metabolome using four mass spectrometry pipelines enhance detection different classes metabolites. This mQTL a wide range metabolites highlighted an...
Abstract Δ22-Unsaturated sterols, containing a double bond at the C-22 position in side chain, occur specifically fungi and plants. Here, we describe identification characterization of cytochrome P450s belonging to CYP710A family as plant desaturase. Recombinant proteins CYP710A1 CYP710A2 from Arabidopsis thaliana CYP710A11 tomato (Lycopersicon esculentum) were expressed using baculovirus/insect system. The exhibited desaturase activity with β-sitosterol produce stigmasterol (CYP710A1, K m =...
Abstract The β-substituted alanine (Ala) synthase (Bsas) family in the large superfamily of pyridoxal 5′-phosphate-dependent enzymes comprises cysteine (Cys) (CSase) [O-acetyl-serine (thiol) lyase] and β-cyano-Ala (CASase) plants. Nine genomic sequences encode putative Bsas proteins Arabidopsis thaliana. physiological roles these isoforms vivo were investigated by characterization T-DNA insertion mutants. Analyses gene expression, activities CSase CASase, levels Cys glutathione bsas mutants...
The degradation pathway of glutathione (GSH) in plants is not well understood. In mammals, GSH predominantly metabolized through the gamma-glutamyl cycle, where degraded by sequential reaction transpeptidase (GGT), cyclotransferase, and 5-oxoprolinase to yield glutamate (Glu) dipeptides that are subject peptidase action. this study, we examined if same Arabidopsis (Arabidopsis thaliana) as occurs mammals. Arabidopsis, oxoprolinase knockout mutants (oxp1-1 oxp1-2) accumulate more 5-oxoproline...
Lysine decarboxylase (LDC) catalyzes the first-step in biosynthetic pathway of quinolizidine alkaloids (QAs), which form a distinct, large family plant alkaloids. A cDNA lysine/ornithine (L/ODC) was isolated by differential transcript screening QA-producing and nonproducing cultivars Lupinus angustifolius. We also obtained L/ODC cDNAs from four other plants, Sophora flavescens, Echinosophora koreensis, Thermopsis chinensis, Baptisia australis. These L/ODCs phylogenetically distinct subclade...
Because cyanobacteria directly harvest CO2 and light energy, their carbon metabolism is important for both basic applied sciences. Here, we show that overexpression of the sigma factor sigE in Synechocystis sp. PCC 6803 widely changes sugar catabolism increases production biodegradable polyester polyhydroxybutyrate (PHB) during nitrogen starvation. elevates levels proteins implicated glycogen catabolism, oxidative pentose phosphate pathway, polyhydroxyalkanoate biosynthesis. PHB accumulation...
As metabolomics can provide a biochemical snapshot of an organism's phenotype it is promising approach for charting the unintended effects genetic modification. A critical obstacle this application inherently limited metabolomic coverage any single analytical platform. We propose using multiple platforms direct acquisition interpretable data set estimable chemical diversity. example, we report our multi-platform that assesses substantial equivalence tomatoes over-expressing taste-modifying...
Sulphonation of small molecules by cytosolic sulphotransferases in mammals is an important process which endogenous are modified for inactivation/activation their biological effects. Plants possess large numbers sulphotransferase genes, but functions largely unknown. Here, we present a functional analysis the Arabidopsis AtSOT12 (At2g03760). gene expression strongly induced salt, and osmotic stress hormone treatments. The T-DNA knock-out mutant sot12 exhibited hypersensitivity to NaCl ABA...
Metabolites in the fruits of edible plants include sweet sugars, visually appealing pigments, various products with human nutritional value, and biologically active plant hormones. Although quantities these metabolites vary during fruit development ripening because cell division enlargement, there are few reports describing actual dynamics changes. Therefore, we applied multiple metabolomic techniques to identify changes metabolite levels pear (Pyrus communis L. ‘La France’). We quantified...
Nitrogen availability is one of the most important factors for survival cyanobacteria. Previous studies on Synechocystis revealed a contradictory situation with regard to metabolism during nitrogen starvation; that is, glycogen accumulated even though expressions sugar catabolic genes were widely upregulated. Here, we conducted transcript and metabolomic analyses using capillary electrophoresis-mass spectrometry sp. PCC 6803 under starvation. The levels some tricarboxylic acid cycle...
Root exudates are derived from plant metabolites and their composition is affected by the nutritional status. The purpose of this study was to examine soybean root under phosphorus (P) deficiency using a metabolite profiling technique. Glycine max L. (cv. Suzuyutaka) grown in culture solution at P concentrations 0 (P0) 8 (P8) mg L−1 for 1, 5, 10 15 days after transplanting. Shoot extract, extract were collected determined capillary electrophoresis/time-of-flight mass spectrometry (CE-TOF...
Rice bran (RB) is a major by-product of rice polishing and rich source bioactive compounds. Here, we investigated the anti-colitis effect diet supplementation with fermented (FRB) in murine model ulcerative colitis. FRB was prepared by dual fermentation RB using fungi lactic acid bacteria. Colitis induced C57Bl/6N male mice (n = 8/group) dextran sodium sulfate (DSS). Body weight change, disease activity index (DAI), histopathology score, tissue myeloperoxidase (MPO) activity, cytokine...
Abstract Response regulators of two-component systems play pivotal roles in the transcriptional regulation responses to environmental signals bacteria. Rre37, an OmpR-type response regulator, is induced by nitrogen depletion unicellular cyanobacterium Synechocystis species PCC 6803. Microarray and quantitative real-time polymerase chain reaction analyses revealed that genes related sugar catabolism metabolism were up-regulated rre37 overexpression. Protein levels GlgP(slr1367), one two...
Abstract Liver fibrosis results from chronic liver injury triggered by factors such as viral infection, excess alcohol intake, and lipid accumulation. However, the mechanisms underlying are not fully understood. Here, we demonstrate that expression of fibroblast growth factor 18 ( Fgf18 ) is elevated in mouse livers following induction models. Deletion hepatocytes attenuates fibrosis; conversely, overexpression promotes fibrosis. Single-cell RNA sequencing reveals an increase number Lrat +...
We have developed a metabolic profiling scheme based on direct-infusion Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS). The consists of: (1) reproducible data collection under optimized FT-ICR/MS analytical conditions; (2) automatic mass-error correction and multivariate analyses for metabolome characterization using newly metabolomics tool (DMASS software); (3) identification of marker metabolite candidates by searching species-metabolite relationship database,...
Background In metabolomics researches using mass spectrometry (MS), systematic searching of high-resolution data against compound databases is often the first step metabolite annotation to determine elemental compositions possessing similar theoretical numbers. However, incorrect hits derived from errors in analyses will be included results composition searches. To assess quality peak information, a novel methodology for false discovery rates (FDR) evaluation presented this study. Based on...
Abstract Site-directed mutagenesis via gene targeting (GT) based on homologous recombination is the ultimate mutation breeding technology because it enables useful information acquired from structural- and computational-based protein engineering to be applied directly molecular breeding, including metabolic engineering, of crops. Here, we employed this rationale introduce precise mutations in OASA2—an α-subunit anthranilate synthase that a key enzyme tryptophan (Trp) biosynthesis rice (Oryza...
Root exudates improve the nutrient acquisition of plants and affect rhizosphere microbial communities. The plant status affects composition root exudates. purpose this study was to examine common bean (Phaseolus vulgaris L.) under phosphorus (P) deficiency using a metabolite profiling technique. Common were grown in culture solution at P concentrations 0 (P0), 1 (P1) 8 (P8) mg L-1 for 1, 10 20 days after transplanting (DAT). collected, their metabolites determined by capillary...