A5028698031- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Surgical Treatments
- Pancreatic and Hepatic Oncology Research
- Metabolomics and Mass Spectrometry Studies
- Gastrointestinal disorders and treatments
- Natural product bioactivities and synthesis
- Colorectal and Anal Carcinomas
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Gastrointestinal Tumor Research and Treatment
- Inflammatory Bowel Disease
- Pancreatitis Pathology and Treatment
- Metastasis and carcinoma case studies
- Antioxidant Activity and Oxidative Stress
- Synthetic Organic Chemistry Methods
- Asymmetric Synthesis and Catalysis
- Microbial Natural Products and Biosynthesis
- Gallbladder and Bile Duct Disorders
- Esophageal and GI Pathology
- Plant Stress Responses and Tolerance
- Liver Disease Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Plant tissue culture and regeneration
Kazusa DNA Research Institute
2015-2025
Chiba Hokusou Hospital
2019-2024
Tohoku University Hospital
2019-2024
Tohoku University
1989-2023
Tohoku Medical and Pharmaceutical University
2022-2023
Tohoku Medical and Pharmaceutical University Hospital
2023
Murata (Japan)
2020-2022
Nippon Medical School
2011-2021
Genomics (United Kingdom)
2018-2020
Tohoku Medical Megabank Organization
2018-2020
Abstract MassBank is the first public repository of mass spectra small chemical compounds for life sciences (<3000 Da). The database contains 605 electron‐ionization spectrometry(EI‐MS), 137 fast atom bombardment MS and 9276 electrospray ionization (ESI)‐MS n data 2337 authentic metabolites, 11 545 EI‐MS 834 other‐MS 10 286 volatile natural synthetic compounds, 3045 ESI‐MS 2 679 drugs contributed by 16 research groups (January 2010). were analyzed under nonstandardized, independent...
Understanding plant metabolism as an integrated system is essential for metabolic engineering aimed at the effective production of compounds useful to human life and global environment. The “omics” approach integrates transcriptome metabolome data into a single set can lead identification unknown genes their regulatory networks involved in pathways interest. One intriguing, although poorly described plants biosynthesis glucosinolates (GSLs), group bioactive secondary products derived from...
Drought is the major environmental threat to agricultural production and distribution worldwide. Adaptation by plants dehydration stress a complex biological process that involves global changes in gene expression metabolite composition. Here, using one type of functional genomics analysis, metabolomics, we characterized metabolic phenotypes Arabidopsis wild-type knockout mutant NCED3 (nc3-2) under stress. plays role dehydration-inducible biosynthesis abscisic acid (ABA), phytohormone...
Abstract Jasmonic acid (JA) and methyl jasmonate (MeJA), collectively known as JAs, regulate diverse physiological processes in plants, including the response to wounding. Recent reports suggest that a cyclopentenone precursor of JA, 12-oxo-phytodienoic (OPDA), can also induce gene expression. However, little is about significance OPDA-dependent We used microarray analysis approximately 21,500 Arabidopsis (Arabidopsis thaliana) genes compare responses MeJA, OPDA treatment. Although many...
Since the completion of genome sequences model organisms, functional identification unknown genes has become a principal challenge in biology. Post-genomics sciences such as transcriptomics, proteomics, and metabolomics are expected to discover gene functions. This report outlines elucidation gene-to-gene metabolite-to-gene networks via integration with transcriptomics presents strategy for novel Metabolomics data Arabidopsis grown under sulfur deficiency were combined analyzed by...
DREB1A/CBF3 and DREB2A are transcription factors that specifically interact with a cis-acting dehydration-responsive element (DRE), which is involved in cold- gene expression Arabidopsis (Arabidopsis thaliana). Overexpression of DREB1A improves stress tolerance to both freezing dehydration transgenic plants. In contrast, overexpression an active form results significant but only slight The downstream products for reported have similar putative functions, genes encoding enzymes carbohydrate...
Summary Jasmonic acid (JA) and methyl jasmonate (MeJA), collectively termed jasmonates, are ubiquitous plant signalling compounds. Several types of stress conditions, such as wounding pathogen infection, cause endogenous JA accumulation the expression jasmonate‐responsive genes. Although jasmonates important components for response in plants, mechanism by which contributes to tolerance has not been clearly defined. A comprehensive analysis jasmonate‐regulated metabolic pathways Arabidopsis...
Summary A large number of metabolites are found in each plant, most which have not yet been identified. Development a methodology is required to deal systematically with unknown metabolites, and elucidate their biological roles an integrated ‘omics’ framework. Here we report the development ‘metabolite annotation’ procedure. The metabolite annotation process by structures functions inferred for metabolites. Tomato ( Solanum lycopersicum cv. Micro‐Tom) was used as model this study using...
Correlations between gene expression and metabolite/phytohormone levels under abiotic stress conditions have been reported for Arabidopsis (Arabidopsis thaliana). However, little is known about these correlations in rice (Oryza sativa 'Nipponbare'), despite its importance as a model monocot. We performed an integrated analysis to clarify the relationships among cold- dehydration-responsive metabolites, phytohormones, transcription rice. An of metabolites indicated that several genes encoding...
P450 CYP722C is a key enzyme catalyzing orobanchol synthesis and undetectable in root exudates after gene knockout.
Summary The saponins of the model legume Medicago truncatula are glycosides at least five different triterpene aglycones: soyasapogenol B, E, medicagenic acid, hederagenin and bayogenin. These aglycones most likely derived from β‐amyrin, a product cyclization 2,3‐oxidosqualene. Mining M. EST data sets led to identification sequences putatively encoding three early enzymes aglycone formation: squalene synthase (SS), epoxidase (SE), β‐amyrin (β‐AS). SS was functionally characterized by...
Abstract Δ22-Unsaturated sterols, containing a double bond at the C-22 position in side chain, occur specifically fungi and plants. Here, we describe identification characterization of cytochrome P450s belonging to CYP710A family as plant desaturase. Recombinant proteins CYP710A1 CYP710A2 from Arabidopsis thaliana CYP710A11 tomato (Lycopersicon esculentum) were expressed using baculovirus/insect system. The exhibited desaturase activity with β-sitosterol produce stigmasterol (CYP710A1, K m =...
Plant activators are compounds, such as analogs of the defense hormone salicylic acid (SA), that protect plants from pathogens by activating plant immune system. Although some have been widely used in agriculture, molecular mechanisms induction largely unknown. Using a newly established high-throughput screening procedure screens for compounds specifically potentiate pathogen-activated cell death Arabidopsis thaliana cultured suspension cells, we identified five prime response. These...
The phytohormone auxin is critical for plant growth and many developmental processes. Members of the P-glycoprotein (PGP/ABCB) subfamily ATP-binding cassette (ABC) transporters have been shown to function in polar movement by transporting over plasma membrane both monocots dicots. Here, we characterize a new Arabidopsis member ABCB subfamily, ABCB21/PGP21, close homolog ABCB4, which conflicting transport directionalities reported. ABCB21 strongly expressed abaxial side cotyledons junctions...
Brassica napus (canola) plants were genetically manipulated to increase the amount and composition of carotenoids in seeds by using seven key enzyme genes involved ketocarotenoid formation, which originated from a soil bacterium Pantoea ananatis (formerly called Erwinia uredovora 20D3), marine bacteria Brevundimonas sp. strain SD212 Paracoccus N81106 Agrobacterium aurantiacum). The gene cassettes, each was surrounded an appropriate promoter terminator, connected tandem manner, resulting...
Abstract Penitrem A is one of the most elaborated members fungal indole diterpenes. Two separate penitrem gene clusters were identified using genomic and RNA sequencing data, 13 out 17 transformations in biosynthesis elucidated by heterologous reconstitution relevant genes. These reactions involve 1) a prenylation‐initiated cationic cyclization to install bicyclo[3.2.0]heptane skeleton (PtmE), 2) two‐step P450‐catalyzed oxidative processes forming unique tricyclic (PtmK PtmU), 3) five...
Abstract The application of DNA array technology and chromatographic separation techniques coupled with mass spectrometry to transcriptomic metabolomic analyses in plants has resulted the generation considerable quantitative data related transcription metabolism. integration “omic” is one major concerns associated research into identifying gene function. Thus, we developed a Web-based tool, KaPPA-View, for representing individual transcripts and/or metabolites on plant metabolic pathway...
Camalexin is the major phytoalexin in Arabidopsis. An almost complete set of camalexin biosynthetic enzymes have been elucidated but only limited information available regarding molecular mechanisms regulating biosynthesis. Here, we demonstrate that ANAC042, a member NAM, ATAF1/2, and CUC2 (NAC) transcription factor family genes, involved biosynthesis induction. T-DNA insertion mutants ANAC042 failed to accumulate at levels achieved wild type, were highly susceptible Alternaria brassicicola...
BackgroundAlthough DNA methylation of colonic mucosa in ulcerative colitis (UC) has been suggested, the majority published reports indicate correlation between colon and occurrence UC-related dysplasia or cancer without considering mucosal inflammatory status. The aim this study was to verify whether inflammation-specific occurs UC.
The Panax genus has been a source of natural medicine, benefitting human health over the ages, among which japonicus represents an important species. Our understanding several key pathways and enzymes involved in biosynthesis ginsenosides, pharmacologically active class metabolites major chemical constituents rhizome extracts from species, are limited. Limited genomic information, lack studies on comparative transcriptomics across species have restricted our biosynthetic mechanisms these...