A5060872382- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Light effects on plants
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Heat shock proteins research
- Plant Gene Expression Analysis
- Seed Germination and Physiology
- Plant responses to water stress
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Soybean genetics and cultivation
- Plant Genetic and Mutation Studies
- Advanced Wireless Communication Techniques
- Research in Cotton Cultivation
- Plant Reproductive Biology
- Physiological and biochemical adaptations
- Plant Water Relations and Carbon Dynamics
- thermodynamics and calorimetric analyses
- Cryptographic Implementations and Security
- Plant Physiology and Cultivation Studies
- Polysaccharides and Plant Cell Walls
- Plant Micronutrient Interactions and Effects
- Lipid metabolism and biosynthesis
- Coding theory and cryptography
Tokyo Institute of Technology
2023-2024
The University of Tokyo
2013-2023
Japan International Research Center for Agricultural Sciences
2008-2013
Kazusa DNA Research Institute
2009
A myriad of drought stress-inducible genes have been reported, and many these are activated by abscisic acid (ABA). In the promoter regions such ABA-regulated genes, conserved cis-elements, designated ABA-responsive elements (ABREs), control gene expression via bZIP-type AREB/ABF transcription factors. Although all three members subfamily, AREB1, AREB2, ABF3, upregulated ABA water stress, it remains unclear whether functional homologs. Here, we report that factors require for full...
ABA is an important phytohormone regulating various plant processes, including stress tolerance, seed development and germination. SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 SRK2I/SnRK2.3 are redundant ABA-activated SNF1-related protein kinases 2 (SnRK2s) in Arabidopsis thaliana. We examined the role of these These SnRK2 proteins were mainly expressed nucleus during The triple mutant (srk2d srk2e srk2i) was sensitive to desiccation showed severe growth defects development. It exhibited a loss...
Responses to water stress are thought be mediated by transcriptional regulation of gene expression via reversible protein phosphorylation events. Previously, we reported that bZIP (basic-domain leucine zipper)-type AREB/ABF (ABA-responsive element-binding protein/factor) transcription factors involved in ABA signaling under conditions Arabidopsis. The AREB1 is phosphorylated vitro ABA-activated SNF1-related kinase 2s (SnRK2s) such as SRK2D/SnRK2.2, SRK2E/SnRK2.6 and SRK2I/SnRK2.3...
Abstract The DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) transcription factor controls water deficit–inducible gene expression and requires posttranslational modification for its activation. activation mechanism is not well understood; however, the stability of this protein in nucleus was recently found to be important Here, we report isolation Arabidopsis thaliana DREB2A-INTERACTING PROTEIN1 (DRIP1) DRIP2, C3HC4 RING domain–containing proteins that interact with DREB2A...
DREB1A/CBF3 and DREB2A are transcription factors that specifically interact with a cis-acting dehydration-responsive element (DRE), which is involved in cold- gene expression Arabidopsis (Arabidopsis thaliana). Overexpression of DREB1A improves stress tolerance to both freezing dehydration transgenic plants. In contrast, overexpression an active form results significant but only slight The downstream products for reported have similar putative functions, genes encoding enzymes carbohydrate...
Summary Soybean ( Glycine max ) is a globally important crop, and its growth yield are severely reduced by abiotic stresses, such as drought, heat, cold. The cis ‐acting element DRE (dehydration‐responsive element)/ CRT plays an role in activating gene expression response to these stresses. Arabidopsis DREB 1/ CBF genes that encode ‐binding proteins function transcriptional activators the cold stress responsive expression. In this study, we identified 14 1‐type transcription factors (Gm 1s)...
Abstract OsTZF1 is a member of the CCCH-type zinc finger gene family in rice (Oryza sativa). Expression was induced by drought, high-salt stress, and hydrogen peroxide. expression also abscisic acid, methyl jasmonate, salicylic acid. Histochemical activity β-glucuronidase transgenic plants containing promoter fused with observed callus, coleoptile, young leaf, panicle tissues. Upon OsTZF1-green fluorescent protein localization cytoplasm cytoplasmic foci. Transgenic overexpressing driven...
In plants, cold temperatures trigger stress responses and long-term that result in tolerance. Arabidopsis thaliana, three dehydration-responsive element (DRE) binding protein 1/C-repeat factors (DREB1/CBFs) act as master switches cold-responsive gene expression. Induction of DREB1 genes triggers the stress-inducible transcriptional cascade, followed by induction numerous function response Many regulatory involved have been identified, but how these orchestrate stress-specific expression...
In order to analyze the molecular mechanisms underlying responses of plants different levels drought stress, we developed a soil matric potential (SMP)-based irrigation system that precisely controls moisture. Using this system, rice seedlings were grown under three levels, denoted Md1, Md2 and Md3, with SMP values set -9.8, -31.0 -309.9 kPa, respectively. Although Md1 treatment did not alter visible phenotype, caused stomatal closure shoot growth retardation (SGR). The Md3 markedly induced...
Abstract Drought and cold represent distinct types of abiotic stress, each initiating unique primary signaling pathways in response to dehydration temperature changes, respectively. However, a convergence at the gene regulatory level is observed where common set stress-responsive genes activated mitigate impacts both stresses. In this review, we explore these intricate networks, illustrating how plants coordinate stress signals into collective transcriptional strategy. We delve molecular...
Abstract Transcription factors of the DRE-Binding1 (DREB1)/C-repeat binding factor family specifically interact with a cis-acting dehydration-responsive element/C-repeat involved in low-temperature stress-responsive gene expression Arabidopsis (Arabidopsis thaliana). Expression DREB1s is induced by low temperatures and regulated circadian clock under unstressed conditions. Promoter sequences contain six conserved motifs, boxes I to VI. We analyzed promoter region DREB1C using transgenic...
Arabidopsis thaliana DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) functions as a transcriptional activator that increases tolerance to osmotic and heat stresses; however, its expression also leads growth retardation reduced reproduction. To avoid these adverse effects, the of DREB2A is predicted be tightly regulated. We identified short promoter region represses under nonstress conditions. Yeast one-hybrid screening for interacting factors GROWTH-REGULATING FACTOR7 (GRF7). GRF7...
Abstract In plants, abiotic stresses induce various physiological changes and growth inhibition that result in adaptive responses to these stresses. However, little is known about how such cause plant inhibition. Many genes have been reported be repressed plants under stress conditions. ZPT2 (for petunia [Petunia hybrida] zinc-finger protein 2)-related proteins with two Cys2/His2-type motifs an ethylene-responsive element binding factor-associated amphiphilic repression motif are thought...
Group A1 heat shock transcription factors (HsfA1s) are the master regulators of stress response (HSR) in plants. Upon shock, HsfA1s trigger a transcriptional cascade that is composed many factors. Despite importance and their downstream acquisition thermotolerance plants, molecular basis activation remains poorly understood. Here, domain analysis HsfA1d, one several Arabidopsis thaliana, demonstrated central region HsfA1d key regulatory represses transactivation activity through interaction...
Soybean (Glycine max) is an important crop around the world. Abiotic stress conditions, such as drought and heat, adversely affect its survival, growth, production. The DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2 (DREB2) group includes transcription factors that contribute to heat tolerance by activating through cis-element dehydration-responsive element (DRE) in response these stimuli. Two modes of regulation, transcriptional posttranslational, are for activation gene expression DREB2A...
Summary Although a variety of transgenic plants that are tolerant to drought stress have been generated, many these show growth retardation. To improve tolerance and plant growth, we applied gene‐stacking approach using two transcription factor genes: DEHYDRATION ‐ RESPONSIVE ELEMENT BINDING 1A ( DREB ) rice PHYTOCHROME INTERACTING FACTOR LIKE 1 Os PIL ). The overexpression has reported in various crops, although it also causes severe dwarf phenotype. is homologue Arabidopsis 4 PIF ,...
The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling Arabidopsis; however, the roles other subclasses remain elusive. Here, we established a double mutant SRK2C/SnR2.8 SRK2F/SnRK2.7 investigate functions II SnRK2s. Microarray analysis suggested that SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) their targets, quantitative reverse...
The mechanisms for plant growth restriction during stress conditions remains unclear. Here, we demonstrate that a phytochrome-interacting factor-like protein, OsPIL1/OsPIL13, acts as key regulator of reduced internode elongation in rice under drought conditions. level OsPIL1 mRNA seedlings grown nonstressed with light/dark cycles oscillated circadian manner peaks the middle light period. Under conditions, expression was inhibited We found highly expressed node portions stem using...
Significance DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2A (DREB2A) is a key transcription factor for plant adaptation to drought and heat. DREB2A activity strictly regulated via proteolysis mediated by the negative regulatory domain (NRD), although molecular basis this regulation has remained unclear decade. We reveal that BTB/POZ AND MATH DOMAIN proteins (BPMs), substrate adaptors Cullin3-based E3 ubiquitin ligase, are long-sought factors responsible NRD-dependent degradation. Through...