A5048621891- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Seed Germination and Physiology
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Soybean genetics and cultivation
- Plant Reproductive Biology
- Plant Water Relations and Carbon Dynamics
- Plant responses to water stress
- CRISPR and Genetic Engineering
- Bone health and treatments
- Silicon Effects in Agriculture
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Plant biochemistry and biosynthesis
- Cancer-related gene regulation
- Light effects on plants
- Biofuel production and bioconversion
- Lipid metabolism and biosynthesis
- Plant responses to elevated CO2
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Polymer-Based Agricultural Enhancements
- RNA Research and Splicing
The University of Tokyo
2013-2024
Swedish University of Agricultural Sciences
2020-2022
RIKEN Center for Sustainable Resource Science
2016-2019
Kumamoto University
1995
Hiroshima University
1995
SUMMARY Global climate change is predicted to result in increased yield losses of agricultural crops caused by environmental conditions. In particular, heat and drought stress are major factors that negatively affect plant development reproduction, previous studies have revealed how these stresses induce responses at physiological molecular levels. Here, we provide a comprehensive overview current knowledge concerning drought, heat, combinations conditions the status plants, including crops,...
DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) is a key transcription factor for drought and heat stress tolerance in Arabidopsis thaliana. DREB2A induces the expression of dehydration- stress-inducible genes under corresponding conditions. Target gene selectivity assumed to require stress-specific posttranslational regulation, but mechanisms this process are not yet understood. Here, we identified DNA POLYMERASE II SUBUNIT B3-1 (DPB3-1), which was previously annotated as NUCLEAR...
Significance The plant hormone abscisic acid (ABA) is essential for drought-stress responses in plants, and its functions have been well studied; however, the detailed molecular mechanisms of ABA biosynthesis during early drought stress need to be further explored. present study identified a transcription factor, NGTHA1 (NGA1), which positively regulates accumulation dehydration by activating NCED3 gene encoding key biosynthetic enzyme. We also cis -acting element bound NGA1 5′ untranslated...
Significance DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2A (DREB2A) is a key transcription factor for plant adaptation to drought and heat. DREB2A activity strictly regulated via proteolysis mediated by the negative regulatory domain (NRD), although molecular basis this regulation has remained unclear decade. We reveal that BTB/POZ AND MATH DOMAIN proteins (BPMs), substrate adaptors Cullin3-based E3 ubiquitin ligase, are long-sought factors responsible NRD-dependent degradation. Through...
Functional diversification of transcription factors allows the precise regulation transcriptomic changes under different environmental conditions. The NUCLEAR FACTOR Y (NF-Y) factor comprises three subunits, NF-YA, NF-YB, and NF-YC, is broadly diversified in plant species, whereas Humans (Homo sapiens) have one protein for each subunit. However, there remains much to be learned about functions subunit plants. Here, we found that NF-YB2 NF-YB3, which greatest sequence similarity other among...
The Arabidopsis thaliana transcription factor DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2A (DREB2A) controls the expression of many genes involved in plant's response to dehydration and heat stress. Despite significance post-translational regulation DREB2A activation, mechanism underlying this activation remains unclear. Here, with aid a newly produced antibody against DREB2A, we characterized stability plants exposed stress stimuli. Endogenous accumulated wild-type subjected A...
The enhancement of heat stress tolerance in crops is an important challenge for food security to facilitate adaptation global warming. In Arabidopsis thaliana, the transcriptional regulator DNA polymerase II subunit B3-1 (DPB3-1)/nuclear factor Y C10 (NF-YC10) has been reported as a positive Dehydration-responsive element binding protein 2A (DREB2A), and overexpression DPB3-1 enhances without growth retardation. Here, we show that interacts with DREB2A homologues rice soya bean....
During de novo plant organ regeneration, auxin induction mediates the formation of a pluripotent cell mass called callus, which regenerates shoots upon cytokinin induction. However, molecular mechanisms underlying transdifferentiation remain unknown. Here, we showed that loss HDA19, histone deacetylase (HDAC) family gene, suppresses shoot regeneration. Treatment with an HDAC inhibitor revealed activity this gene is essential for Further, identified target genes whose expression was regulated...
The subnuclear distribution of centromeres is cooperatively regulated by condensin II and the linker nucleoskeleton cytoskeleton (LINC) complex. However, other nuclear membrane structures proteins are probably involved in centromere dynamics distribution. Here, we focused on pore complex (NPC), which known to regulate gene expression, transcription memory, chromatin structure addition transport between cytoplasm nucleoplasm. We report here that some nucleoporins (Nups), including Nup85,...
Abstract The endosperm is a nutritive tissue supporting embryo growth in flowering plants. Most commonly, the initially develops as coenocyte (multinucleate cell) and then cellularizes. This process of cellularization frequently disrupted hybrid seeds generated by crosses between different plant species or plants that differ ploidy, resulting arrest seed lethality. reason for upon failure remains unclear. In this study, we show triploid Arabidopsis thaliana embryos surrounded uncellularized...
Polycomb Repressive Complex 2 (PRC2)-mediated trimethylation of histone H3 on lysine 27 (H3K27me3) and methylation 3 9 (H3K9me) are two repressive epigenetic modifications that typically localized in distinct regions the genome. For reasons unknown, however, they co-occur some organisms special tissue types. In this study, we show maternal alleles marked by H3K27me3 Arabidopsis endosperm were targeted demethylase REF6 became activated during germination. contrast, H3K27me3, H3K9me2, CHG...
We report a 39-year-old Japanese man with HDL and apoA-I deficiency as well data from members of his family. Corneal opacity stomatocyte were found but not tonsillar hypertrophy, xanthomas, or splenomegaly. His serum cholesterol, apoA-I, apoA-II, LDL cholesterol levels t mg/dL, < 3 6 175 respectively. Plasma triglyceride, phospholipid, apoB, apoC-III, apoE all within normal limits. Lecithin:cholesterol acyltransferase activity was half normal, while lipoprotein lipase hepatic triglyceride...
The Polycomb Repressive Complex 2 (PRC2) is well-known for its role in controlling developmental transitions by suppressing the premature expression of key regulators. Previous work revealed that PRC2 also controls onset senescence, a form programmed cell death (PCD) plants. Whether induction PCD response to stress similarly suppressed remained largely unknown. In this study, we explored whether triggered immunity- and disease-promoting pathogen effectors associated with changes distribution...
Abstract Seed development in angiosperms starts with double fertilization, where two paternal sperm cells fertilize the maternal gametes. This leads to formation of embryo and endosperm. These fertilization products are enveloped by maternally-derived seed coat, which is inhibited prior Polycomb Repressive Complex 2 (PRC2). complex deposits repressive histone mark H3K27me3, whose removal necessary for coat formation. Here, we show that JUMONJI-type (JMJ) demethylases expressed coats...
Gene editing technology based on the clustered regularly interspaced short palindromic repeats (CRISPR) system has garnered widespread use in plant genomes because of its cost-effectiveness, efficiency, and simplicity. To avoid integration foreign genes any DNA fragments into target cell genomes, researchers have developed a that introduces vitro-assembled ribonucleoproteins (RNPs) consisting guide RNA (gRNA) Cas protein cells, enabling direct genome editing. This was designed to deliver...
Abstract Polycomb Repressive Complex 2 (PRC2)-mediated trimethylation of histone H3 on lysine 27 (H3K27me3) and methylation 3 9 (H3K9me) are two repressive epigenetic modifications that typically localized in distinct regions the genome. For reasons unknown, however, they co-occur some organisms special tissue types. In this study, we show maternal alleles marked by H3K27me3 Arabidopsis endosperm were targeted demethylase REF6 became activated during germination. contrast, H3K27me3, H3K9me2,...