A5085284499- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Lipid metabolism and biosynthesis
- Plant-Microbe Interactions and Immunity
- Peroxisome Proliferator-Activated Receptors
- ATP Synthase and ATPases Research
- Endoplasmic Reticulum Stress and Disease
- Nuclear Structure and Function
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Light effects on plants
- RNA Research and Splicing
- Plant biochemistry and biosynthesis
- Genomics and Chromatin Dynamics
- Advanced Proteomics Techniques and Applications
- Plant responses to water stress
- 14-3-3 protein interactions
- RNA and protein synthesis mechanisms
Ritsumeikan University
2016-2025
Nara Institute of Science and Technology
2012-2022
Takayama Red Cross Hospital
2015
Japan Atomic Energy Agency
2012
Northwest African American Museum
2010-2011
Nagoya University
2011
Tokyo Gakugei University
2011
Kyoto University
2004-2008
University of Zurich
2005
Japan Science and Technology Agency
2004
The Arabidopsis thaliana K+ channel KAT1 has been suggested to have a key role in mediating the aperture of stomata pores on surface plant leaves. Although activity is thought be regulated by phosphorylation, endogenous pathway and primary target site for this modification remained unknown. In present study, we demonstrated that C-terminal region acts as phosphorylation calcium-independent ABA (abscisic acid)-activated protein kinase SnRK2.6 (Snf1-related 2.6). This was confirmed LC-MS/MS...
Abstract The nuclear pore complex (NPC) facilitates nucleocytoplasmic transport, a crucial process for various cellular activities. NPC comprises ~30 nucleoporins and is well characterized in vertebrates yeast. However, only eight plant have been identified, little information available about the complete molecular structure of NPCs. In this study, an interactive proteomic approach was used to identify Arabidopsis thaliana nucleoporins. A series five cycles analysis performed using green...
Perception of microbe-associated molecular patterns by host cell surface pattern recognition receptors (PRRs) triggers the intracellular activation mitogen-activated protein kinase (MAPK) cascades. However, it is not known how PRRs transmit immune signals to MAPK cascades in plants. Here, we identify a complete phospho-signaling transduction pathway from PRR-mediated pathogen We found that receptor-like cytoplasmic PBL27 connects chitin receptor complex CERK1-LYK5 and cascade. interacts with...
Plant survival requires the ability to acclimate heat. When plants are subjected heat shock, expression of various genes is induced, and become tolerant higher temperatures. We found that transient treatment with geldanamycin radicicol, two shock protein 90 (HSP90) inhibitors, induced heat-inducible acclimation in Arabidopsis thaliana seedlings. Heat reduced activity exogenously expressed glucocorticoid receptor (GR). Since GR depends on HSP90, this suggests reduces cytosolic HSP90 vivo....
In higher plants, the chloroplast NAD(P)H dehydrogenase (NDH) complex mediates photosystem I (PSI) cyclic and chlororespiratory electron transport. We reported previously that NDH interacts with PSI to form a supercomplex (NDH-PSI). this study, NDH18 FKBP16-2 (FK506 Binding Protein 16-2), detected in NDH-PSI by mass spectrometry, were shown be subunits analysis of their knockdown lines. On basis extensive mutant characterization, we propose structural model for NDH, whereby is divided into...
Here we show that related isoform AtPGP4 is expressed predominantly during early root development. loss‐of‐function plants reveal enhanced lateral initiation and hair lengths both known to be under the control of auxin. Further, atpgp4 altered sensitivities toward auxin transport inhibitor, NPA. Finally, mutant roots elevated free levels reduced capacities. These results together with yeast growth assays suggest a direct involvement in processes controlling
Some subunits of chloroplast NAD(P)H dehydrogenase (NDH) are related to those the respiratory complex I, and NDH mediates photosystem I (PSI) cyclic electron flow. Despite extensive surveys, donor its binding have not been identified. Here, we identified three novel components required for activity. CRRJ CRRL J- J-like proteins, respectively, subcomplex A. CRR31 is an Src homology 3 domain-like fold protein, C-terminal region may form a tertiary structure similar that PsaE, ferredoxin (Fd)...
Oil bodies in seeds of higher plants are surrounded with oleosins. Here we demonstrate a novel role for oleosins protecting oilseeds against freeze/thaw-induced damage their cells. We detected four oil isolated from Arabidopsis thaliana, and designated them OLE1, OLE2, OLE3 OLE4 decreasing order abundance the seeds. For reverse genetics, oleosin-deficient mutants (ole1, ole2, ole3 ole4) generated three double (ole1 ole1 ole2 ole3). Electron microscopy showed an inverse relationship between...
Abstract The micronutrient zinc is essential for all living organisms, but it toxic at high concentrations. Here, to understand the effects of excess on plant cells, we performed an iTRAQ (for isobaric tags relative and absolute quantification)-based quantitative proteomics approach analyze microsomal proteins from Arabidopsis (Arabidopsis thaliana) roots. Our was sensitive enough identify 521 proteins, including several membrane proteins. Among them, IRT1, iron transporter, FRO2, a...
Microdomains in the plasma membrane (PM) have been proposed to be involved many important cellular events plant cells. To understand role of PM microdomains cold acclimation, we isolated as detergent-resistant fractions (DRMs) from Arabidopsis seedlings and compared lipid protein compositions before after acclimation. The DRM was enriched sterols glucocerebrosides, proportion free increased protein-to-lipid ratio greater than that total fraction. amount recovered DRMs decreased gradually...
Numerous plant defense-related proteins are thought to congregate in plasma membrane microdomains, which consist mainly of sphingolipids and sterols. However, the extent microdomains contribute defense responses plants is unclear. To elucidate relationship between innate immunity rice (Oryza sativa), we established lines levels containing 2-hydroxy fatty acids were decreased by knocking down two genes encoding acid 2-hydroxylases (FAH1 FAH2) demonstrated that less abundant these lines. By...
Significance Hormone-like peptides derived from small coding genes (<100 amino acids) have not been extensively characterized in relation to abiotic stress tolerance. Focusing on 17 salinity stress-inducible Arabidopsis , we showed that four conferred increased tolerance when overexpressed transgenic plants. One of the ( AtPROPEP3 ) was found induce by treatment with a 13-peptide (KPTPSSGKGGKHN) fragment, providing unique functional evidence for enhanced plants response peptide treatment....
The plasma membrane-associated Ca(2+)-binding protein-2 of Arabidopsis thaliana is involved in the growth root hair tips. Several transgenic lines that overexpress 23 residue N-terminal domain this protein under control hair-specific EXPANSIN A7 promoter lack hairs completely. role normal and stress conditions was examined one these hair-less (NR23). Compared with wild type, NR23 showed a 47% reduction water absorption, decreased drought tolerance, lower ability to adapt heat. Growth...
The adaptor protein-2 (AP-2) complex is a heterotetramer involved in clathrin-mediated endocytosis of cargo proteins from the plasma membrane animal cells. homologous genes AP-2 subunits are present genomes plants; however, their identities and roles endocytic pathways not clearly defined plants. Here, we reveal molecular composition Arabidopsis thaliana its dynamics on membrane. We identified all α-, β-, σ-, μ-subunits detected weak interaction with clathrin heavy chain. μ-subunit protein...
Oil bodies are intracellular structures present in the seed and leaf cells of many land plants. Seed oil known to function as storage compartments for lipids. However, physiological is unknown. Here, we show that subcellular factories production a stable phytoalexin response fungal infection senescence. Proteomic analysis prepared from Arabidopsis (Arabidopsis thaliana) leaves identified caleosin (CLO3) α-dioxygenase (α-DOX1). Both CLO3 α-DOX1 were localized on surface bodies. Infection with...
In many plant species, roots maintain specific growth angles relative to the direction of gravity, known as gravitropic set point (GSAs). These contribute efficient acquisition water and nutrients. AtLAZY1/LAZY1-LIKE (LZY) genes are involved in GSA control by regulating auxin flow toward gravity Arabidopsis. Here, we demonstrate that RCC1-like domain (RLD) proteins, identified LZY interactors, essential regulators polar transport. We show interaction CCL with BRX RLD is important for...
Membrane trafficking in plants is involved cellular development and the adaptation to various environmental changes. SNARE (soluble N-ethylmaleimide-sensitive factor attachment receptor) proteins mediate fusion between vesicles organelles facilitate transport cargo cells. To characterize further protein networks cells, we carried out interactome analysis of using 12 transgenic Arabidopsis thaliana expressing green fluorescent (GFP)-tagged Qa-SNAREs (SYP111, SYP121, SYP122, SYP123, SYP132,...
Over-reduction of the photosynthetic electron transport (PET) chain should be avoided, because accumulation reducing carriers produces reactive oxygen species (ROS) within photosystem I (PSI) in thylakoid membranes and causes oxidative damage to chloroplasts. To prevent production ROS H+ gradient (ΔpH) needs built up across suppress over-reduction state PET chain. In this study, we aimed identify critical component that stimulates ΔpH formation under illumination higher plants. do this,...
Rett syndrome (RTT) is a neurodevelopmental disorder caused by MECP2 mutations. Although emerging evidence suggests that MeCP2 deficiency associated with dysregulation of mechanistic target rapamycin (mTOR), which functions as hub for various signaling pathways, the mechanism underlying this association and molecular pathophysiology RTT remain elusive. We show here promotes posttranscriptional processing particular microRNAs (miRNAs) component microprocessor Drosha complex. Among...
Significance DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2A (DREB2A) is a key transcription factor for plant adaptation to drought and heat. DREB2A activity strictly regulated via proteolysis mediated by the negative regulatory domain (NRD), although molecular basis this regulation has remained unclear decade. We reveal that BTB/POZ AND MATH DOMAIN proteins (BPMs), substrate adaptors Cullin3-based E3 ubiquitin ligase, are long-sought factors responsible NRD-dependent degradation. Through...
Plant growth and architecture is regulated by the polar distribution of hormone auxin. Polarity flexibility this process provided constant cycling auxin transporter vesicles along actin filaments, coordinated a positive auxin-actin feedback loop. Both transport vesicle are inhibited synthetic inhibitors, such as 1-N-naphthylphthalamic acid (NPA), counteracting effect auxin; however, underlying targets mechanisms unclear. Using NMR, we map NPA binding surface on Arabidopsis thaliana ABCB...
Summary The balance between carbon (C) and nitrogen (N) availability is an important determinant for various phases of plant growth; however, the detailed mechanisms regulating C/N response are not well understood. We previously described two related ubiquitin ligases, ATL31 ATL6, that function in Arabidopsis thaliana . Here, we used FLAG tag affinity purification MS analysis to identify proteins targeted by ATL31, thus likely be involved phase transition checkpoint based on status. This...