A5065533748- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Enzyme Production and Characterization
- Ubiquitin and proteasome pathways
- Plant Stress Responses and Tolerance
- Rice Cultivation and Yield Improvement
- Phytase and its Applications
- Plant responses to water stress
- GABA and Rice Research
- Mycobacterium research and diagnosis
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Tuberculosis Research and Epidemiology
- Plant-Microbe Interactions and Immunity
- Endoplasmic Reticulum Stress and Disease
- Food composition and properties
- Cytomegalovirus and herpesvirus research
- Biofuel production and bioconversion
- 14-3-3 protein interactions
- Plant Gene Expression Analysis
- Herpesvirus Infections and Treatments
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Viral-associated cancers and disorders
Hokkaido University
2015-2025
Hokkaido University of Science
2022
Juntendo University
2022
Fracture Analysis Consultants (United States)
2016
Hokkaido Sapporo Kita High School
2015
Gunma Industrial Technology Center
2013
Nagoya University
1992-2008
Kindai University
1985-2007
Columbia University
2003-2006
Japan Science and Technology Agency
2005-2006
The rice slender mutant (slr1-1) is caused by a single recessive mutation and results in constitutive gibberellin (GA) response phenotype. elongates as if saturated with GAs. In this mutant, (1) elongation was unaffected an inhibitor of GA biosynthesis, (2) GA-inducible α-amylase produced the aleurone layers without gibberellic acid application, (3) endogenous content lower than wild-type plant. These indicate that product SLR1 gene intermediate signal transduction pathway. maps to OsGAI has...
A subline of the P3 (Jijoye) Burkitt lymphoma cell line, designated P3HR-1, initially contained 1 to 5% cells which were positive by indirect immunofluorescence with selected human sera. After 4 months propagation, this line regularly showed 15 40% reactive cells. Antigen(s) in was similar or identical that found several other tumor lines previous studies. When incubated at 35 32 C for 9 days without refeeding, more than 50% became immunofluorescence-positive. Thirteen different cultures...
To study the regulation of ammonium uptake into rice roots, three transporter genes (OsAMT1;1, 1;2 and 1;3; Oryza sativa transporter) were isolated examined. OsAMT1s belong to AMT1 family, containing 11 putative transmembrane-spanning domains. Southern blot analysis screening genome database confirmed that with OsAMT1;1–1;3 complete family had been isolated. Heterologous expression in yeast Saccharomyces cerevisiae mutant 31019b showed all exhibit transport activity. Northern a distinct...
Increasing evidence shows that sugars can act as signals affecting plant metabolism and development. Some of the effects on growth development suggest an interaction sugar with hormonal regulation. We investigated induction [alpha]-amylase by gibberellic acid in barley embryos aleurone layers. Our results show signaling interact regulation acid-induced gene expression grains. The layer is unaffected presence sugars, but repression carbohydrates effective embryo. [alpha]-Amylase embryo...
To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of an ammonium-uptake-defective yeast mutant showed that this gene encodes functional transporters. was constitutively expressed both roots shoots irrespective the supply inorganic nitrogen medium, whereas OsAMT3;1 expression relatively weak. A database search amino acid sequence there are 10 putative OsAMT genes i.e. three each for...
An adequate carbohydrate supply contributes to the survival of seeds under conditions limited oxygen availability. The amount soluble, readily fermentable carbohydrates in dry cereal is usually very limited, with starch representing main storage compound. Starch breakdown during germination result action hydrolytic enzymes and only through concerted [alpha]-amylase (EC 3.2.1.1), [beta]-amylase 3.2.1.2), debranching enzyme 3.2.1.41), [alpha]-glucosidase 3.2.1.20) can be hydrolyzed completely....
Significance Nitrogen availability has a large impact on plant biomass and crop production. Both depletion excessive application of nitrogen affect yields quality disturb important ecosystems. Thus, understanding the mechanisms by which regulates developmental transitions affecting reproduction, such as flowering, is to sustainably improve yields. Our study identified FBH4 key transcription factor mediating nitrogen-responsive flowering in Arabidopsis . levels control phosphorylation state...
The three members of the rice OsAMT1 gene family ammonium transporters show distinct expression patterns; constitutive and ammonium-promoted in shoots roots for OsAMT1;1; root-specific ammonium-inducible OsAMT1;2; nitrogen-repressible OsAMT1;3 [Sonoda et al. (2003), Plant Cell Physiol. 44: 726]. To clarify feedback mechanisms, to identify regulatory factors genes, accumulation mRNAs its dependence on endogenous nitrogen compounds (as quantified by capillary electrophoresis) was studied....
Summary Plants are able to sense and respond changes in the balance between carbon (C) nitrogen (N) metabolite availability, known as C/N response. During transition photoautotrophic growth following germination, of seedlings is arrested if a high external ratio detected. To clarify mechanisms for sensing signaling during this period, we screened large collection FOX transgenic plants, overexpressing full‐length cDNAs, individuals continue post‐germinative under severe stress. One line,...
Summary The balance between carbon (C) and nitrogen (N) availability is an important determinant for various phases of plant growth; however, the detailed mechanisms regulating C/N response are not well understood. We previously described two related ubiquitin ligases, ATL31 ATL6, that function in Arabidopsis thaliana . Here, we used FLAG tag affinity purification MS analysis to identify proteins targeted by ATL31, thus likely be involved phase transition checkpoint based on status. This...
Abstract Silk is a protein fiber used to weave fabrics and as biomaterial in medical applications. Recently, genetically modified silks have been produced from transgenic silkworms. In the present study, silkworms for mass production of three colors fluorescent silks, (green, red, orange) are generated using vector originating fibroin H chain gene classical breeding method. The suitability recombinant making investigated by harvesting large amounts cocoons, obtained rearing over 20 thousand...
Carbon (C) and nitrogen (N) are essential elements for metabolism, their availability, called the C/N balance, must be tightly coordinated optimal growth in plants. Previously, we have identified ubiquitin ligase CNI1/ATL31 as a novel regulator by screening plants grown on stress medium containing excess sugar limited N. To elucidate further effect of balance plant to determine physiological function ATL31, performed response analysis using an atmospheric CO2 manipulation system. Under...
Apolipoprotein B100 (apoB) is a large (520-kDa) complex secretory protein; its secretion regulated posttranscriptionally by several degradation pathways. The best described of these degradative processes co-translational ubiquitinylation and proteasomal nascent apoB, involving the 70- 90-kDa heat shock proteins multiple components pathway. Ubiquitinylation involves proteins, including ligases called E3s, that coordinate covalent binding ubiquitin to target proteins. recent discovery tumor...
We investigated sugar uptake and transport in rice (Oryza sativa) embryo during grain germination. Endogenous levels, accumulation of starch granules, gene expression a sucrose transporter (OsSUT1) were examined using embryos germinated with or without exogenous supply. Starch granules remarkably accumulated the cells around vascular bundles as consequence taken up by embryos, indicating that taken-up sugars are transiently converted into starch. In situ detection for OsSUT1 mRNA indicated...
To clarify the processes involved in plant immunity, we have isolated and characterized a single recessive Arabidopsis mutant, cad1 (constitutively activated cell death 1), which shows phenotype that mimics lesions seen hypersensitive response (HR). This mutant spontaneously expression of pathogenesis-related (PR) genes, leading to 32-fold increase salicylic acid (SA). Inoculation plants with Pseudomonas syringae pv tomato DC3000 mutation results restriction bacterial growth. Cloning CAD1...
The gibberellin‐inducible rice α‐amylase gene, RAmy1A , was demonstrated to be sugar repressed in embryos and functional dissection of the promoter relation its sugar‐modulated expression performed. Gibberellin‐response cis ‐elements GARE (TAACAAA) pyrimidine box (CCTTTT) were partially involved repression.