A5063952388- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Enzyme Production and Characterization
- GABA and Rice Research
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Food composition and properties
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Biofuel production and bioconversion
- Retinal Diseases and Treatments
- Plant Disease Resistance and Genetics
- Toxin Mechanisms and Immunotoxins
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Superconducting Materials and Applications
- Plant biochemistry and biosynthesis
- Retinal Imaging and Analysis
- Plant Molecular Biology Research
- Phytase and its Applications
- Plant Virus Research Studies
Kindai University
2014-2025
National Institutes for Quantum Science and Technology
2022-2024
Insight (China)
2024
Nara Institute of Science and Technology
2007-2018
Graduate School USA
2012-2013
Ritsumeikan University
2011
Laboratory of Molecular Genetics
2007
Japan Research Institute
2005
Kyoto University
2005
Yokohama City University
2003
Abstract Reactive oxygen species (ROS) produced by NADPH oxidase play critical roles in various cellular activities, including plant innate immunity response. In contrast with the large multiprotein complex of phagocytes, plants, only homologs catalytic subunit gp91phox and cytosolic regulator small GTPase Rac are found. Plant known as Rboh (for respiratory burst homolog). Although numerous have been isolated regulation enzymatic activity remains unknown. All rboh genes identified to date...
OsRac1, one of the Rac/Rop family small GTPases, plays important roles in defense responses, including a role production reactive oxygen species mediated by NADPH oxidase. We have identified an effector namely rice ( Oryza sativa ) cinnamoyl-CoA reductase 1 (OsCCR1), enzyme involved lignin biosynthesis. Lignin, which is polymerized through peroxidase activity using H 2 O cell wall, factor plant because it presents undegradable mechanical barrier to most pathogens. Expression OsCCR1 was...
Perception of microbe-associated molecular patterns by host cell surface pattern recognition receptors (PRRs) triggers the intracellular activation mitogen-activated protein kinase (MAPK) cascades. However, it is not known how PRRs transmit immune signals to MAPK cascades in plants. Here, we identify a complete phospho-signaling transduction pathway from PRR-mediated pathogen We found that receptor-like cytoplasmic PBL27 connects chitin receptor complex CERK1-LYK5 and cascade. interacts with...
Cell death plays important roles in the development and defense of plants as other multicellular organisms. Rapid production reactive oxygen species often is associated with plant against pathogens, but their molecular mechanisms are not known. We introduced constitutively active dominant negative forms small GTP-binding protein OsRac1 , a rice homolog human Rac, into wild type lesion mimic mutant analyzed H 2 O cell transformed cultures plants. The results indicate that Rac regulator well rice.
Production of reactive oxygen intermediates (ROI) and a form programmed cell death called hypersensitive response (HR) are often associated with disease resistance plants. We have previously shown that the Rac homolog rice, OsRac1, is regulator ROI production in rice. Here we show constitutively active OsRac1 (i) causes HR-like responses greatly reduces lesions against virulent race rice blast fungus; (ii) bacterial blight; (iii) enhanced phytoalexin alters expression defense-related genes....
Metallothioneins are small, ubiquitous Cys-rich proteins known to be involved in reactive oxygen species (ROS) scavenging and metal homeostasis. We found that the expression of a metallothionein gene (OsMT2b) was synergically down-regulated by OsRac1 rice (Oryza sativa) blast-derived elicitors. Transgenic plants overexpressing OsMT2b showed increased susceptibility bacterial blight blast fungus. OsMT2b-overexpressing cells reduced elicitor-induced hydrogen peroxide production. In contrast,...
We used rice dwarf1 ( d1 ) mutants lacking a single-copy Gα gene and addressed Gα's role in disease resistance. exhibited highly reduced hypersensitive response to infection by an avirulent race of blast. Activation PR expression the leaves infected with blast was delayed for 24 h relative wild type. H 2 O production induced sphingolipid elicitors (SE) were strongly suppressed cell cultures. Expression constitutively active OsRac1, small GTPase Rac rice, restored SE-dependent defense...
This study describes the effect of starch-synthesizing enzymes on biosynthesis storage starch in rice amylose-extender mutants, which contain branched D-glucans with abnormal structures. Western blot analysis indicated that two out five mutant lines lacked an isoform branching enzyme, termed RBE3, although levels granule-bound synthase and a major form RBE1, were normal these mutants. Proteins corresponding to 87-kDa RBE3 molecule present three other mutants as well wild type. However, level...
Summary We screened 93 lesion mimic mutants of rice for resistance to the blast fungus, Magnaporthe grisea , and found eight that exhibited significant fungus. called these cdr (cell death resistance) further analyzed three them. Two mutations, cdr1 cdr2 were recessive one, Cdr3 was dominant. Many small brownish lesions developed over entire leaf 20–50 days after sowing. TUNEL staining revealed DNA fragmentation occurred in blade cells homozygous mutants. Autofluorescence callose deposition...
Abstract A small GTPase, Rac1, plays a key role in rice (Oryza sativa) innate immunity as part of complex regulatory proteins. Here, we used affinity column chromatography to identify RACK1 (for Receptor for Activated C-Kinase 1) an interactor with Rac1. functions various mammalian signaling pathways and is involved hormone development plants. Rice contains two genes, RACK1A RACK1B, the protein interacts GTP form Rac1 positively regulates at both transcriptional posttranscriptional levels....
Serotonin is a well known neurotransmitter in mammals and plays an important role various mental functions humans. In plants, the serotonin biosynthesis pathway its function are not understood. The rice sekiguchi lesion (sl) mutants accumulate tryptamine, candidate substrate for biosynthesis. We isolated SL gene by map-based cloning found that it encodes CYP71P1 cytochrome P450 monooxygenase family. A recombinant protein exhibited tryptamine 5-hydroxylase enzyme activity catalyzed conversion...
Abstract Mitogen-activated protein kinase (MAPK) cascades are activated in plants during responses to pathogens or pathogen-derived elicitors and mediate intracellular stress responses. Here, we show that a rice (Oryza sativa) MAPK, OsMAPK6, was posttranslationally cell culture by sphingolipid elicitor. Suppression of OsMAPK6 expression RNA interference resulted strong reduction pathogen-induced Phe ammonia-lyase mRNA, whereas the mRNA level another OsMAPK5a, highly increased. Silencing...
Recognition of microbe-associated molecular patterns (MAMPs) initiates pattern-triggered immunity in host plants. Pattern recognition receptors (PRRs) and receptor-like cytoplasmic kinases (RLCKs) are the major components required for sensing transduction these patterns. However, regulation RLCKs by PRRs their specificity remain obscure. In this study we show that PBL27, an Arabidopsis ortholog OsRLCK185, is immediate downstream component chitin receptor CERK1 contributes to chitin-induced...
Abstract A rice (Oryza sativa) Rac/Rop GTPase, Os Rac1, is involved in innate immunity, but its molecular function largely unknown. RAR1 (for required for Mla12 resistance) and HSP90 (a heat shock protein 90 kD) are important components of R gene–mediated disease resistance, their conserved several plant species. has also recently been shown to be mammalian immunity. However, functions at the level not well understood. In this study, we examined functional relationships between RAR1, HSP90....
Plant NADPH oxidases (Rboh, for respiratory burst oxidase homolog) produce reactive oxygen species that are key regulators of various cellular events including plant innate immunity. Rbohs possess a highly conserved cytoplasmic N-terminal region containing two EF-hand motifs regulate Rboh activity. Rice (Oryza sativa) RbohB (OsRbohB) is regulated by the direct binding small GTPase (Rac1) to this regulatory as well Ca(2+) EF-hands. Here, we present atomic structure OsRbohB. The reveals...
Plant-specific Rac/Rop small GTPases function as molecular switches for numerous signal transduction events, including defense responses. To understand the of each seven family members in rice, we studied tissue-specific expression patterns genes by semi-quantitative reverse transcription–PCR (RT–PCR), and also subcellular localization using green fluorescent protein (GFP) fusion proteins transient systems. We investigated roles these disease resistance testing single Rac/Rop-RNAi (RNA...
Perception of microbe-associated molecular patterns (MAMPs) including chitin by pattern recognition receptors (PRRs) rapidly induces activation mitogen-activated protein kinase (MAPK) cascades. However, how PRRs transmit immune signals to the MAPK cascade is largely unknown. Recently, Arabidopsis receptor-like cytoplasmic PBL27 has been reported activate MAPKs through phosphorylation AtMAPKKK5 in signaling pathway. In this study, we found that OsRLCK185, a rice ortholog PBL27, regulates...
The U-box type ubiquitin ligase PUB44 positively regulates pattern-triggered immunity in rice. Here, we identify PBI1, a protein that interacts with PUB44. Crystal structure analysis indicates PBI1 forms four-helix bundle structure. also WRKY45, master transcriptional activator of rice immunity, and negatively its activity. is degraded upon perception chitin, this suppressed by silencing or expression XopP, indicating degradation depends on These data suggest suppresses WRKY45 activity when...
Suc, an end product of photosynthesis, is metabolized by Suc synthase in sink organs as initial step the biosynthesis storage products. activity known to be regulated reversible phosphorylation, but details this process are unclear at present. Rice SPK, a calcium-dependent protein kinase, expressed uniquely endosperm immature seed, and its involvement biosynthetic pathways products was suggested. Antisense SPK transformants lacked ability accumulate such starch, produced watery seed with...
Summary The Arabidopsis RPM1 protein confers resistance to disease caused by Pseudomonas syringae strains delivering either the AvrRpm1 or AvrB type III effector proteins into host cells. We characterized two closely related RPM1‐interacting proteins, RIN2 and RIN3. RIN3 encode RING‐finger ubiquitin ligases with six apparent transmembrane domains an ubiquitin‐binding CUE domain. are orthologs of mammalian autocrine motility factor receptor, a cytokine receptor localized in both plasma...