A5083341947- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Fungal and yeast genetics research
- Biochemical and Structural Characterization
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Insect Resistance and Genetics
- GABA and Rice Research
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Phytase and its Applications
- Enzyme Structure and Function
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Plant nutrient uptake and metabolism
- Proteins in Food Systems
- Natural product bioactivities and synthesis
- Enzyme Production and Characterization
- Viral Infectious Diseases and Gene Expression in Insects
- Plant biochemistry and biosynthesis
National Agriculture and Food Research Organization
2025
Institute of Agrobiological Sciences
1999-2025
Institute of Crop Science
2018-2020
Iwate University
2019
Nagoya University
1999-2017
Fukui Prefectural University
2000-2012
Nara Women's University
2005
Hokkaido University
2003
Kyushu University
2002
Nara Institute of Science and Technology
2002
We have characterized a rice (Oryza sativa) dwarf mutant, dwarf11 (d11), that bears seeds of reduced length. To understand the mechanism by which seed length is regulated, D11 gene was isolated map-based cloning method. The found to encode novel cytochrome P450 (CYP724B1), showed homology enzymes involved in brassinosteroid (BR) biosynthesis. phenotype d11 mutants restored application brassinolide (BL). Compared with wild-type plants, aberrant mRNA accumulated at higher levels and...
Previously, we reported that the rice dwarf mutant, d1 , is defective in α subunit of heterotrimeric G protein (Gα). In present study, gibberellin (GA) signaling and role Gα GA-signaling pathway were investigated. Compared with wild type, GA induction α-amylase activity aleurone cells was greatly reduced. Relative to 3 -treated layer had lower expression Ramy1A which encodes α-amylase, OsGAMYB a GA-inducible transcriptional factor, no increase Ca 2 + -ATPase . However, presence high...
Transgenic rice containing an antisense cDNA for the α subunit of heterotrimeric G protein produced little or no mRNA and exhibited abnormal morphology, including dwarf traits setting small seeds. In normal rice, was abundant in internodes florets, tissues closely related to abnormality transformants. The position α-subunit gene mapped on chromosome 5 by mapping with restriction fragment length polymorphism. linked locus a mutant, Daikoku ( d-1 ), which is known exhibit phenotypes similar...
We used rice dwarf1 ( d1 ) mutants lacking a single-copy Gα gene and addressed Gα's role in disease resistance. exhibited highly reduced hypersensitive response to infection by an avirulent race of blast. Activation PR expression the leaves infected with blast was delayed for 24 h relative wild type. H 2 O production induced sphingolipid elicitors (SE) were strongly suppressed cell cultures. Expression constitutively active OsRac1, small GTPase Rac rice, restored SE-dependent defense...
In the present study, we investigated function of heterotrimeric G protein β-subunit (Gβ) gene (RGB1) in rice. RGB1 knock-down lines were generated wild type and d1-5, a mutant deficient for α-subunit (Gα) (RGA1). Both transgenic showed browning lamina joint regions nodes that could be attributed to reduction function, as abnormality was not observed d1-5. The d1-5 shorter, suggesting positive regulator cellular proliferation, addition RGA1. number sterile seeds also increased both lines....
Abstract Triterpenoid saponins are specialised metabolites distributed widely in the plant kingdom that consist of one or more sugar moieties attached to triterpenoid aglycones. Despite accepted view glycosylation is catalysed by UDP-dependent glycosyltransferase (UGT), UGT which catalyses transfer conserved glucuronic acid moiety at C-3 position glycyrrhizin and various soyasaponins has not been determined. Here, we report a cellulose synthase superfamily-derived (CSyGT) 3- O-...
Summary Two genes in the rice genome were identified as those encoding γ subunits, γ1 and γ2, of heterotrimeric G proteins. Using antibodies against recombinant proteins for α, β, γ1, γ2 subunits protein complexes, all proven to be localized plasma membrane rice. Gel filtration solubilized showed that α present large complexes (about 400 kDa) containing other probably also some proteins, whereas amounts β (γ1 γ2) freed from took a 60‐kDa form. A yeast two‐hybrid assay co‐immunoprecipitation...
The α subunit of plant heterotrimeric G proteins (Gα) plays pivotal roles in multiple aspects development and responses to hormones. Recently, several lines evidence have shown that Gα participates brassinosteroid (BR) Arabidopsis rice plants. In this study, we conducted a comprehensive analysis the BR using defective mutant gene, T65d1. Decreased sensitivity 24-epi-brassinolide (24-epiBL) T65d1 was observed many processes examined, e.g. inhibition root growth promotion coleoptile...
<title>Abstract</title> Soybeans fix atmospheric N<sub>2</sub> through symbiosis with rhizobia, N<sub>2</sub>-fixing bacteria. The relationship between rhizobia and soybeans, particularly those high nitrous oxide (N<sub>2</sub>O)-reducing activities (Nos<sup>++</sup>), can be used for reducing N<sub>2</sub>O emissions from agricultural soils. However, inoculating soybeans Nos<sup>++</sup> under field conditions often fails because of the competition indigenous Nos<sup>−</sup> (no...
We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm mixed Escherichia coli strains. By simply coculturing plasmid-free F(-) strain and another harboring plasmid on antibiotic-free agar media, transformed cells were produced within 24-48 h at frequency 10(-10)-10(-9) per recipient cell. PCR analysis demonstrated occurrence transfer. These survived until least day 7 under conditions. Liquid cultures same strains Luria-Bertani broth no or few transformants,...
Magnaporthe oryzae, the fungus causing rice blast disease, should contend with host innate immunity to develop invasive hyphae (IH) within living cells. However, molecular strategies establish biotrophic interactions are largely unknown. Here, we report biological function of a M. oryzae-specific gene, Required-for-Focal-BIC-Formation 1 (RBF1). RBF1 expression was induced in appressoria and IH only when inoculated plant tissues. Long-term successive imaging live cell fluorescence revealed...
Inducible resistance to macrolide, lincosamide, and streptogramin type B antibiotics in Streptomyces spp. comprises a family of diverse phenotypes which characteristic subsets the macrolide-lincosamide-streptogramin induce mediated by mono- or dimethylation adenine, both, 23S ribosomal ribonucleic acid. In these studies, patterns induction specificity associated acid changes are described. fradiae NRRL 2702 erythromycin induced vernamycin B, whereas hygroscopicus IFO 12995, reverse was...
It has been shown that the disruption of α-subunit gene heterotorimeric G-proteins (Gα) results in dwarf traits, erection leaves and setting small seeds rice. These mutants are called d1. We have studied expression profiles transcripts translation products rice Gα ten alleles d1 including five additional newly identified. By RT-PCR, were detected all alleles. western blot, proteins not plasma membrane fractions with exception d1-4. In d1-4, one amino acid change GTP-binding box A protein was...
Abstract Lotus japonicus is an important model legume plant in several fields of research, such as secondary (specialized) metabolism and symbiotic nodulation. This accumulates triterpenoids; however, less information regarding its composition, content biosynthesis available compared with Medicago truncatula Glycine max. In this study, we analyzed the triterpenoid composition L. japonicus. accumulated C-28-oxidized triterpenoids (ursolic, betulinic oleanolic acids) soyasapogenols...
Soyasaponins are specialized metabolites present in soybean seeds that affect the taste and quality of soy-based foods. The composition sugar chains attached to aglycone moiety soyasaponins is regulated by genetic loci such as sg-1, sg-3 sg-4. Here, we report cloning characterization Sg-3 gene, which responsible for conjugating terminal (third) glucose (Glc) at C-3 chain soyasaponins. gene Glyma.10G104700 disabled cultivar, 'Mikuriya-ao', due deletion genomic DNA results absence a Glc...
The d1 mutant, which is deficient for the heterotrimeric G-protein α subunit (Gα) gene of rice, shows dwarfism and sets small round seeds. To determine whether in due to a reduction cell number or shortened length, leaf sheath, internode, root lemma was compared between Nipponbare, wild-type rice d1-5, allele derived from Nipponbare. Our results indicate that reduced all organs analyzed d1-5. In addition, enlargement found roots although organ length d1-5 shorter than rice. These suggest Gα...
We used site-directed mutagenesis to engineer two constitutively active forms of the alpha subunit a rice heterotrimeric G protein. The recombinant proteins produced from these novel cDNAs had GTP-binding activity but no GTPase activity. A chimeric gene for form was introduced into mutant d1, which is defective alpha-subunit gene. All transformants essentially showed wild-type phenotype compared with normal cultivars, although seed sizes were substantially increased and internode lengths...
Abstract The rice dwarf1 ( d1 ) mutant, which lacks the α subunit of a heterotrimeric G protein (Gα protein), shows abnormal morphology due to shortened internodes, dark green leaves and grains that are small round. Proteome analysis was used in this study aid determining function Gα embryos. Using 2‐DE, seven seed embryo proteins were shown be down‐regulated mutant as compared with its wild type. These included receptor for activated C‐kinase (RACK) six globulin‐2 (REG2). REG2 have similar...
We show that a rice GRAS family protein, CIGR2, is bonafide transcriptional activator, and through this function, targets the B-type heat shock protein-encoding gene OsHsf23 (Os09g0456800). CIGR2 (Os07g0583600) an N-acetylchitooligosaccharide elicitor-responsive whose activity, direct control of OsHsf23, required for mediating hypersensitive cell death activation during pathogen infection. RNAi lines similarly exhibited higher level granulation in epidermal cells leaf sheath inoculated with...
We present evidence that the rice receptor for N ‐acetylchitooligosaccharide elicitor does not couple to heterotrimeric G‐protein (G‐protein), one of most important signal transducers from cell surface down‐stream effectors in various cellular responses many organisms. Using mutant cells lacking functional α‐subunit, suspension‐cultured derived Daikoku dwarf ( d1 ) mutants were shown contain mutations coding region α‐subunit (Fujisawa et al. 1999) (oligochitin) compared with those...
Triterpenes (C30) constitute one of the diverse class natural products with potential applications in food, cosmetic and pharmaceutical industries. Soyasaponins are oleanane-type triterpenoids widespread among legumes particularly abundant soybean seeds. They have associated various pharmacological implications undesirable taste properties soybean-based food products. Uncovering biosynthetic genes soyasaponins will provide new opportunities to control pathway for human benefits. However,...