A5011192571- CCD and CMOS Imaging Sensors
- Legume Nitrogen Fixing Symbiosis
- Infrared Target Detection Methodologies
- Plant nutrient uptake and metabolism
- Image Processing Techniques and Applications
- Nematode management and characterization studies
- Thin-Film Transistor Technologies
- Agronomic Practices and Intercropping Systems
- Advanced Optical Sensing Technologies
- Telecommunications and Broadcasting Technologies
- Advanced Optical Imaging Technologies
- Constructed Wetlands for Wastewater Treatment
- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Cassava research and cyanide
- Plant-Microbe Interactions and Immunity
- Image Enhancement Techniques
- Coastal wetland ecosystem dynamics
- Soybean genetics and cultivation
- Analytical Chemistry and Sensors
- Optical Systems and Laser Technology
- Transgenic Plants and Applications
- Image and Video Quality Assessment
- Advanced Measurement and Detection Methods
- Noise Effects and Management
Obihiro University of Agriculture and Veterinary Medicine
2023-2025
Tohoku University
2007-2023
University of Minnesota
2008-2019
Biotechnology Institute
2010-2019
Hokkaido University
2013-2018
Network Group (Czechia)
2017
Japan Science and Technology Agency
2014-2015
Twin Cities Orthopedics
2013
Advanced Dermatology
2011
University of Missouri
2010
Genome-wide association study (GWAS) has revolutionized the search for genetic basis of complex traits. To date, GWAS have generally relied on relatively sparse sampling nucleotide diversity, which is likely to bias results by preferentially high-frequency SNPs not in complete linkage disequilibrium (LD) with causative SNPs. avoid these limitations we conducted >6 million identified sequencing genomes 226 accessions model legume Medicago truncatula. We used data identify candidate genes and...
Abstract Background The sinorhizobia are amongst the most well studied members of nitrogen-fixing root nodule bacteria and contribute substantial amounts fixed nitrogen to biosphere. While alfalfa symbiont Sinorhizobium meliloti RM 1021 was one first rhizobial strains be completely sequenced, little information is available about genomes this large diverse species group. Results Here we report draft assembly annotation 48 comprising five genospecies. S. medicae taxonomically related, they...
Abstract Nodulation of soybean (Glycine max) root hairs by the nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum is a complex process coordinated mutual exchange diffusible signal molecules. A metabolomic study was performed to identify small molecules produced in roots and during rhizobial infection process. Metabolites extracted from mock inoculated or with B. were analyzed gas chromatography-mass spectrometry ultraperformance liquid chromatography-quadrupole time flight-mass...
The slanted-edge method specified in ISO Standard 12233, which measures the modulation transfer function (MTF) by analyzing an image of a slightly slanted knife-edge target, is not robust against noise because it takes derivative each data line edge-angle estimation. We propose here modified that estimates edge angle fitting two-dimensional to data. has higher accuracy, precision, and robustness than 12233 applicable any arbitrary pixel array, enabling multidirectional MTF estimate single...
Genotype-specific incompatibility in legume-rhizobium symbiosis has been suggested to be controlled by effector-triggered immunity underlying pathogenic host-bacteria interactions. However, the rhizobial determinant interacting with host resistance protein (e.g., Rj2) and molecular mechanism of symbiotic remain unclear. Using natural mutants Bradyrhizobium diazoefficiens USDA 122, we identified a type III-secretory NopP as Rj2-soybean. The analysis nopP mutations variants culture collection...
An increasing number of outbreaks gastroenteritis recently caused by Escherichia coli O157:H7 have been linked to the consumption leafy green vegetables. Although it is known that E. survives and grows in phyllosphere lettuce plants, molecular mechanisms which this bacterium associates with plants are largely unknown. The goal study was identify genes relevant its interaction, survival, or attachment leaf surfaces, comparing K-12, a model system, O157:H7, pathogen associated large outbreaks....
The symbiosis between rhizobial bacteria and legume plants has served as a model for investigating the genetics of nitrogen fixation evolution facultative mutualism. We used deep sequence coverage (>100×) to characterize genomic diversity at nucleotide level among 12 Sinorhizobium medicae 32 S. meliloti strains. Although these species are closely related share host plants, based on ratio shared polymorphisms fixed differences we found that horizontal gene transfer (HGT) was confined almost...
An ultrahigh-definition video system is considered to be a prospective candidate for future broadcasting services providing high sensation of reality. This paper will discuss the design considerations in construction an experimental with 4000 scanning lines. The parameters were determined based on relationship between reality and viewing angle. camera was developed four-CCD (charge-coupled device) image-acquisition technology: two 2.5-in. 8M-pixel CCDs are used green, one each red blue....
Trehalose, a disaccharide accumulated by many microorganisms, acts as protectant during periods of physiological stress, such salinity and desiccation. Previous studies reported that the trehalose biosynthetic genes (otsA, treS, treY) in Bradyrhizobium japonicum were induced desiccation stresses. Functional mutational analyses indicated disruption otsA decreased accumulation cells an treY double mutant extremely low level trehalose. In contrast, to greater extent treS mutant, maltose levels...
ABSTRACT It was previously demonstrated that there are no indigenous strains of Bradyrhizobium japonicum forming nitrogen-fixing root nodule symbioses with soybean plants in arable field soils Poland. However, bacteria currently classified within this species present (together canariense ) as populations specific for nodulation legumes the Genisteae tribe. These rhizobia, infecting such lupins, well established Polish soils. The studies described here were based on experiments, at Poznań...
Gibberellic acid (GA3), indole-3-acetic (IAA), salicylic (SA), 1-aminocyclopropane-1-carboxylic (ACC) and aminoethoxyvinylglycine (AVG) are popular endogenous growth regulators of plants. However, the effects their exogenous addition on biomass production aquatic plants, including Lemnoidae "duckweeds," largely unknown. In this study, Lemna minor was tested for 10 d in Hoagland medium containing each compound at different concentrations 0−50 µM. GA3, IAA, SA were found to have no apparent...
N2O is an important greenhouse gas influencing global warming, and agricultural land the predominant (anthropogenic) source of emissions. Here, we report high N2O-reducing activity Bradyrhizobium ottawaense, suggesting potential for efficiently mitigating emission from lands. Among 15 B. ottawaense isolates examined, activities most (13) strains were approximately five-fold higher than that diazoefficiens USDA110T under anaerobic conditions. This robust was confirmed by reductase (NosZ)...
<title>Abstract</title> Soybeans fix atmospheric N<sub>2</sub> through symbiosis with rhizobia, N<sub>2</sub>-fixing bacteria. The relationship between rhizobia and soybeans, particularly those high nitrous oxide (N<sub>2</sub>O)-reducing activities (Nos<sup>++</sup>), can be used for reducing N<sub>2</sub>O emissions from agricultural soils. However, inoculating soybeans Nos<sup>++</sup> under field conditions often fails because of the competition indigenous Nos<sup>−</sup> (no...
rRNA is essential for life because of its functional importance in protein synthesis. The (rrn) operon encoding 16S, 23S, and 5S rRNAs located on the "main" chromosome all bacteria documented to date frequently used as a marker chromosomes. Here, our genome analysis plant-associated alphaproteobacterium, Aureimonas sp. AU20, indicates that this strain has sole rrn small (9.4 kb), high-copy-number replicon. We designated unusual replicon carrying background an rrn-lacking (RLC) rrn-plasmid....
Although microbial inoculation may be effective for sustainable crop production, detrimental aspects have been argued because of the potential inoculated microorganisms to behave as invaders and negatively affect ecosystem. We herein compared impact rhizobial on soil bacterial community with that agricultural land-use changes using a 16S rRNA amplicon ana-lysis. Soybean plants were cultivated without five types bradyrhizobial inoculants (Bradyrhizobium diazoefficiens or Bradyrhizobium...
Nitrogen-fixing nodules are formed as a result of series interactions between rhizobia and leguminous plants. Bradyrhizobium elkanii produces rhizobitoxine, an enol-ether amino acid, which has been regarded phytotoxin because it causes chlorosis in soybeans. However, recent studies have revealed that rhizobitoxine plays positive role establishing symbiosis B. host legumes: enhances the nodulation process competitiveness by inhibiting ethylene biosynthesis roots. In addition, gene for...
The ultrahigh-definition television (UDTV) camera system requires an image sensor having four times higher resolution and two frame rate than the conventional HDTV systems. Also, with a small optical format low power consumption is required for practical UDTV To respond to these requirements, we have developed 8.3-M-pixel digital-output CMOS active pixel (APS) application. It features of 1.25inch, less 600 mW at dark, while reproducing low-noise, 60-frames/s progressive scan image. equipped...
Bradyrhizobium japonicum is a facultative chemoautotroph capable of utilizing hydrogen gas as an electron donor in respiratory chain terminated by oxygen to provide energy for cellular processes and carbon dioxide assimilation via reductive pentose phosphate pathway. A transcriptomic analysis B. cultured chemoautotrophically identified 1,485 transcripts, representing 17.5% the genome, differentially expressed when compared heterotrophic cultures. Genetic determinants required utilization...
ABSTRACT Agrobacterium -mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the transfer. To suppress evolution, we introduced 1-aminocyclopropane-1-carboxylate (ACC) deaminase into tumefaciens . The ACC enhanced A. plants.
Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and ottawaense were isolated from the roots field-grown sorghum plants in Fukushima, classified into "Rhizobia" with nodulated soybeans, "Free-living diazotrophs", "Non-diazotrophs" by nitrogen fixation nodulation assays. Genome analyses revealed that possessed genes for N2O reduction, but lacked those Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing...
Symbiosis between organisms influences their evolution via adaptive changes in genome architectures. Immunity of soybean carrying the Rj2 allele is triggered by NopP (type III secretion system [T3SS]-dependent effector), encoded symbiosis island A (SymA) B. diazoefficiens USDA122. This immunity was overcome many mutants with large SymA deletions that encompassed T3SS (rhc) and N2 fixation (nif) genes were bounded insertion sequence (IS) copies direct orientation, indicating homologous...
An amplified MOS imager (AMI) for use in each pixel a high-speed TV camera is discussed. The AMI performs such functions as amplification, readout, and resetting independently completely. Thus the easier to operate completely free from image lag, has relatively little smear (2*10/sup -5/) compared with other devices. can also handle both hole electron signals when photoconductive layer laminated on its surface. area dimensions are 8.8 mm (H)*6.6 (V) 510 (H)*490 pixels. Each an FET amplifier...
Aims The objective of this study was to examine transcriptional changes in Escherichia coli when the bacterium growing lettuce rhizoshpere. Methods and Results A combination microarray analyses, colonization assays confocal microscopy used gain a more complete understanding bacterial genes involved growth E. K12 root rhizosphere using novel hydroponic assay system. After 3 days interaction with roots, protein synthesis, stress responses attachment were up-regulated. Mutants curli production...