A5048175681- EEG and Brain-Computer Interfaces
- Chemical Synthesis and Analysis
- Molecular spectroscopy and chirality
- Functional Brain Connectivity Studies
- Protein Structure and Dynamics
- Visual perception and processing mechanisms
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- Neural dynamics and brain function
- Glycosylation and Glycoproteins Research
- HIV Research and Treatment
- Plant nutrient uptake and metabolism
- Linguistics and Language Studies
- Neurobiology and Insect Physiology Research
- Analytical Chemistry and Chromatography
- Silk-based biomaterials and applications
- Legume Nitrogen Fixing Symbiosis
- DNA and Nucleic Acid Chemistry
- Enzyme Structure and Function
- Plant Molecular Biology Research
- Biochemical Analysis and Sensing Techniques
- Neural Networks and Applications
- Neuropeptides and Animal Physiology
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
Kyushu Institute of Technology
2013-2024
Advanced Analysis Center
2016-2024
National Agriculture and Food Research Organization
2016-2023
Institute of Agrobiological Sciences
2006-2016
Peptide Institute (Japan)
2009
Fukuoka Institute of Technology
2009
RIKEN Center for Integrative Medical Sciences
2008
Waseda University
2007
NEC (Japan)
1991-2007
Cisco Systems (China)
2006
The complex and coordinated regulation of flowering has high ecological agricultural significance. maturity locus E1 a large impact on time in soybean, but the molecular basis for is largely unknown. Through positional cloning, we delimited to 17.4-kb region containing an intron-free gene ( ). protein contains putative bipartite nuclear localization signal distantly related B3 domain. In recessive allele, nonsynonymous substitution occurred signal, leading loss specificity earlier flowering....
Significance Genetically improving the root system architectures of plants is an effective strategy for developing climate-resilient crops. In this study, we revealed that a cloned rice quantitative trait locus associated with growth angle, qSOR1 , DRO1 homolog involved in gravitropic responses. The loss-of-function allele qsor1 resulted roots developed on soil surface and enabled to avoid reducing stress found saline paddy soils and, consequently, increased yields. We show homologs could be...
The B motif is a signature of type-B response regulators (ARRs) involved in His-to-Asp phosphorelay signal transduction systems Arabidopsis. Homologous motifs occur widely the GARP family plant transcription factors. To gain general insight into structure and function (or motifs), we characterized derived from representative ARR, ARR10, which led to number intriguing findings. First, ARR10 (named ARR10-B extending Thr-179 Ser-242) possesses nuclear localization signal, as indicated by...
Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is central negative regulator response in germination; however, the molecular function AHG1 remain elusive. Here we report that interacts with DELAY OF (DOG1), which pivotal positive dormancy. DOG1 acts upstream impairs...
In order to improve the design of HIV-1 protease inhibitors, it is essential understand how they interact with active site residues, particularly catalytic Asp25 and Asp125 residues. KNI-272 a promising, potent inhibitor (Ki ≈ 5 pM), currently undergoing phase 1 clinical trials. Because asymmetric, complex forms homodimeric also lacks symmetry, two monomers can have distinct NMR spectra. Monomer specific signal assignments were obtained for amino acid residues in drug binding as well six...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNMR and X-ray Evidence That the HIV Protease Catalytic Aspartyl Groups Are Protonated in Complex Formed by a Non-Peptide Cyclic Urea-Based InhibitorToshimasa Yamazaki, Linda K. Nicholson, Paul Wingfield, Stephen J. Stahl, Joshua D. Kaufman, Charles Eyermann, C. Nicholas Hodge, Patrick Y. S. Lam, A. Torchia, Cite this: Am. Chem. Soc. 1994, 116, 23, 10791–10792Publication Date (Print):November 1, 1994Publication History Published online1 May...
Significance Worker ants are responsible for various tasks their colony. In chemical communication, odorant-binding proteins and chemosensory proteins, which accumulate in the sensillum lymph antennae, play roles transporting semiochemicals to receptors. However, number of these is not sufficient interact with a large semiochemicals. Niemann–Pick type C2 protein was identified from antenna worker Japanese carpenter ant, Camponotus japonicus (CjapNPC2). CjapNPC2 accumulated cavity basiconic...
Abstract Ca2+/calmodulin (CaM)–dependent protein kinase (CCaMK) is a key regulator of root nodule and arbuscular mycorrhizal symbioses believed to be decoder for Ca2+ signals induced by microbial symbionts. However, it unclear how CCaMK activated these microbes. Here, we investigated in vivo activation symbiotic signaling, focusing mainly on the significance epistatic relationships among functional domains CCaMK. Loss-of-function mutations EF-hand motifs revealed critical importance third EF...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA topochemical approach to explain morphiceptin bioactivityToshimasa Yamazaki, Seonggu Ro, Murray Goodman, Nga N. Chung, and Peter W. SchillerCite this: J. Med. Chem. 1993, 36, 6, 708–719Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/jm00058a007https://doi.org/10.1021/jm00058a007research-articleACS PublicationsRequest reuse permissionsArticle...
Juvenile hormone (JH) plays crucial roles in many aspects of the insect life. All JH actions are initiated by transport hemolymph as a complex with JH-binding protein (JHBP) to target tissues. Here, we report structural mechanism delivery JHBP based upon crystal and solution structures apo JH-bound JHBP. In solution, apo-JHBP exists equilibrium multiple conformations different orientations gate helix for hormone-binding pocket ranging from closed open forms. gate-open form results fully...
Dihydroflavonol 4-reductase (DFR) is the key enzyme committed to anthocyanin and proanthocyanidin biosynthesis in flavonoid biosynthetic pathway. DFR proteins can catalyse mainly three substrates (dihydrokaempferol, dihydroquercetin, dihydromyricetin), show different substrate preferences. Although relationships between preference amino acids region responsible for specificity have been investigated several plant species, molecular basis of not yet fully understood.By using degenerate...
The silk fibroin stored in the gland of Bombyx mori silkworm, called "liquid silk", is spun out and converted into fiber with extremely high strength toughness. Therefore it important to determine structure before spinning Silk I at an atomic level clarify formation mechanism. We proposed repeated type II β-turn as solid state model peptide (AG)15 several NMR techniques previously. In this paper, solution native silk" was determined NMR, especially for tandem sequences (GAGXGA)n (X = S, Y,...
Flowering time is one of the most important agronomic traits in rice (Oryza sativa L.), because it defines harvest seasons and cultivation areas, affects yields. We used a map-based strategy to clone Heading date 18 (Hd18). The difference flowering between Japanese cultivars Koshihikari Hayamasari was due single nucleotide polymorphism within Hd18 gene, which encodes an amine oxidase domain-containing protein homologous Arabidopsis FLOWERING LOCUS D (FLD). allele knockdown gene expression...
N2O is an important greenhouse gas influencing global warming, and agricultural land the predominant (anthropogenic) source of emissions. Here, we report high N2O-reducing activity Bradyrhizobium ottawaense, suggesting potential for efficiently mitigating emission from lands. Among 15 B. ottawaense isolates examined, activities most (13) strains were approximately five-fold higher than that diazoefficiens USDA110T under anaerobic conditions. This robust was confirmed by reductase (NosZ)...
NMR studies of the binding a substrate to an inactive HIV-1 protease construct, containing active site mutation PR(D25N), are reported. Substrate titration measurements monitored by HSQC spectra and (15)N-edited NOESY experiment show that chromogenic analog capsid/p2 cleavage binds PR(D25N) with equilibrium dissociation constant, K(D), 0.27 +/- 0.05 mM, upper limits association rate constants, 2 x 10(4) M(-1)s(-1) 10 s(-1), respectively, at 20 degrees C, pH 5.8. This constant is not in...
Abstract The three‐dimensional solution structure of the HIV‐1 protease homodimer, MW 22.2 kDa, complexed to a potent, cyclic urea‐based inhibitor, DMP323, is reported. This first an HIV protease/inhibitor complex that has been elucidated. Multidimensional heteronuclear NMR spectra were used assemble more than 4,200 distance and angle constraints. Using constraints, together with hybrid geometry/simulated annealing protocol, ensemble 28 structures was calculated having no or violations...
Previously, we isolated a 4‐kDa peptide capable of binding to 43‐kDa receptor‐like protein and stimulating kinase activity the in soybean. Both them were found localize plasma membranes cell walls. Here, report physiological effects expressed transiently cultured carrot bird's‐foot trefoil cells transfected with pBI 121 plasmid containing gene. At early developmental stage, transgenic callus grew rapidly compared wild both species. Cell proliferation vitro nonembryogenic was apparently...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDeuterium NMR analysis of poly(.gamma.-benzyl L-glutamate) in the lyotropic liquid-crystalline state: orientational order .alpha.-helical backbone and conformation pendant side chainAkihiro Abe Toshimasa YamazakiCite this: Macromolecules 1989, 22, 5, 2138–2145Publication Date (Print):May 1, 1989Publication History Published online1 May 2002Published inissue 1...
We report the synthesis, biological activity, and conformational analysis of tetrapeptide analogs related to [Val4]morphiceptin [D-Val4]morphiceptin in which proline at second position has been replaced with cis-2-aminocyclopentane carboxylic acid (cis-2-Ac5c). Since cis-2-Ac5c residue contains a normal amide, only trans form observed about amide bond between first residues. The is beta amino two chiral centers resulting possible configurational isomers, namely (1S, 2R) 1R, 2S) forms....
Leguminous plants establish endosymbiotic associations with rhizobia and form root nodules in which the fix atmospheric nitrogen. The host plant intracellular strictly control this symbiotic nitrogen fixation. We recently reported a Lotus japonicus Fix − mutant, apn1 ( aspartic peptidase nodule-induced 1 ), that impairs APN1 encodes nodule-specific involved phenotype rhizobial strain-specific manner. This host-strain specificity implies some molecular interactions between factors are...