A5019747610- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Bacteriophages and microbial interactions
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Polymer Nanocomposite Synthesis and Irradiation
- Glycosylation and Glycoproteins Research
- NMR spectroscopy and applications
- Ubiquitin and proteasome pathways
- Transgenic Plants and Applications
- Ion channel regulation and function
- Plant Stress Responses and Tolerance
- Polymer Nanocomposites and Properties
- Metalloenzymes and iron-sulfur proteins
- Plant tissue culture and regeneration
- Insect-Plant Interactions and Control
- Plant Pathogenic Bacteria Studies
- Carbohydrate Chemistry and Synthesis
- Polysaccharides and Plant Cell Walls
Toyo University
2023-2025
Advanced Analysis Center
2018-2023
National Agriculture and Food Research Organization
2018-2021
Institute of Agrobiological Sciences
2007-2016
Osaka University
2007-2014
SPring-8
2007-2014
Protein Research Foundation
2014
Kindai University
2011
Kyushu Tokai University
2011
Osaka Prefecture University
2011
Summary Three gibberellin (GA) receptor genes ( AtGID1a , AtGID1b and AtGID1c ), each an ortholog of the rice GA gene OsGID1 were cloned from Arabidopsis, characteristics their recombinant proteins examined. The GA‐binding activities three confirmed by in vitro assay. Biochemical analyses revealed similar ligand selectivity among recombinants, all recombinants showed higher affinity to 4 than other GAs. was unique its binding pH dependence when compared with two, only showing a narrow range...
Abstract GIBBERELLIN INSENSITIVE DWARF1 (GID1) encodes a soluble gibberellin (GA) receptor that shares sequence similarity with hormone-sensitive lipase (HSL). Previously, yeast two-hybrid (Y2H) assay revealed the GID1-GA complex directly interacts SLENDER RICE1 (SLR1), DELLA repressor protein in GA signaling. Here, we demonstrated, by pull-down and bimolecular fluorescence complementation (BiFC) experiments, GA-dependent GID1–SLR1 interaction also occurs planta. GA4 was found to have...
The B motif is a signature of type-B response regulators (ARRs) involved in His-to-Asp phosphorelay signal transduction systems Arabidopsis. Homologous motifs occur widely the GARP family plant transcription factors. To gain general insight into structure and function (or motifs), we characterized derived from representative ARR, ARR10, which led to number intriguing findings. First, ARR10 (named ARR10-B extending Thr-179 Ser-242) possesses nuclear localization signal, as indicated by...
In rice (Oryza sativa) and Arabidopsis thaliana, gibberellin (GA) signaling is mediated by GIBBERELLIN-INSENSITIVE DWARF1 (GID1) DELLA proteins in collaboration with a GA-specific F-box protein. To explore when plants evolved the ability to perceive GA GID1/DELLA pathway, we examined these components lycophyte Selaginella moellendorffii bryophyte Physcomitrella patens. An silico search identified several homologs of GID1, DELLA, GID2, protein rice, both species. Sm GID1a GID1b, GID1 from S....
Abstract Plasticity of root growth in response to environmental cues and stresses is a fundamental characteristic land plants. However, the molecular basis underlying regulation under stressful conditions poorly understood. Here, we report that rice nuclear factor, RICE SALT SENSITIVE3 (RSS3), regulates cell elongation during adaptation salinity. Loss function RSS3 only moderately inhibits normal conditions, but it provokes spontaneous swelling, accompanied by severe inhibition, saline...
Summary Plants are able to sense and respond changes in the balance between carbon (C) nitrogen (N) metabolite availability, known as C/N response. During transition photoautotrophic growth following germination, of seedlings is arrested if a high external ratio detected. To clarify mechanisms for sensing signaling during this period, we screened large collection FOX transgenic plants, overexpressing full‐length cDNAs, individuals continue post‐germinative under severe stress. One line,...
The LysM domain probably binds peptidoglycans, but how it does so has yet to be described. For this report, we measured the thermal stabilities of recombinant domains derived from Pteris ryukyuensis chitinase-A (PrChi-A) and monitored their binding N-acetylglucosamine oligomers ((GlcNAc)n) using differential scanning calorimetry, isothermal titration NMR spectroscopy. We thereby characterized certain domains' functional structural features. observed that are very resistant denaturation...
A glycosylation island is a genetic region required for glycosylation. The of flagellin in Pseudomonas syringae pv. tabaci 6605 consists three orfs: orf1, orf2 and orf3. Orf1 encode putative glycosyltransferases, their deletion mutants, Deltaorf1 Deltaorf2, exhibit deficient or produce partially glycosylated respectively. Digestion from wild-type bacteria non-glycosylated mutant using aspartic N-peptidase subsequent HPLC analysis revealed candidate amino acids. By generation site-directed...
Tomato mosaic virus (ToMV), like other eukaryotic positive-strand RNA viruses, replicates its genomic in replication complexes formed on intracellular membranes. Previous studies showed that a host seven-pass transmembrane protein TOM1 is necessary for efficient ToMV multiplication. Here, we show small GTP-binding ARL8, along with TOM1, co-purified FLAG epitope-tagged 180K from solubilized membranes of ToMV-infected tobacco (Nicotiana tabacum) cells. When cells expressed FLAG-tagged ARL8...
Abstract Conferring drought resistant traits to crops is one of the major aims current breeding programs in response global climate changes. We previously showed that exogenous application acetic acid roots various plants could induce increased survivability under subsequent stress conditions, but details metabolism exogenously applied acid, and nature signals induced by its application, have not been unveiled. In this study, we show rice rapidly induces jasmonate signaling upon resulting...
An indole-3-acetic acid (IAA)-glucose hydrolase, THOUSAND-GRAIN WEIGHT 6 (TGW6), negatively regulates rice grain weight and starch accumulation before heading. A 1-bp deletion in tgw6 results loss of function enhances size yield. Thus, TGW6 has been a target for breeding strains with increased Although the effect on sink well understood, its impact source ability (the to produce carbohydrates from leaves supply organs, referred as shoot carbohydrate here) unclear. Here, we investigated...
NMR studies of the binding a substrate to an inactive HIV-1 protease construct, containing active site mutation PR(D25N), are reported. Substrate titration measurements monitored by HSQC spectra and (15)N-edited NOESY experiment show that chromogenic analog capsid/p2 cleavage binds PR(D25N) with equilibrium dissociation constant, K(D), 0.27 +/- 0.05 mM, upper limits association rate constants, 2 x 10(4) M(-1)s(-1) 10 s(-1), respectively, at 20 degrees C, pH 5.8. This constant is not in...
Many seed storage proteins, including monomeric 2S albumin and polymeric prolamin, contain conserved sequences in three separate regions, termed A, B, C, which the consensus motifs LxxC, CCxQL, PxxC, respectively. Protein-sorting mechanisms rice (Oryza sativa) endosperm were studied with a green fluorescent protein (GFP) fused to different segments of α-globulin, monomeric, ABC-containing protein. The whole ABC region together GFP was efficiently transported vacuoles (type II bodies [PB-II])...
Summary Rice EL5 is an ATL family gene characterized by a transmembrane domain at the N‐terminal and RING‐H2 finger (RFD), which exhibits ubiquitin ligase (E3) activity. To elucidate physiological roles of EL5, we analyzed transgenic rice plants overexpressing mutant proteins that are impaired in E3 activity to various degrees. Plants expressing EL5C153A EL5W165A , encode inactive E3, showed rootless phenotype accompanied cell death root primordia, those EL5V162A with moderately activity,...
Summary In Arabidopsis, three receptors exist for the phytohormone gibberellin. Of three, only a double loss‐of‐function mutant ( atgid1a atgid1c ) shows dwarf phenotype, while other and all single mutants show no abnormality in height. this study we that expression of AtGID1b–GUS mRNA, driven by AtGID1b promoter, is low inflorescence stems, but may be 10% AtGID1a–GUS AtGID1a promoter. However, – GUS enzymatic activity does not them. This factor strongly suggests lacks sufficient protein...
The genomes of the Tomato mosaic virus and many other plant animal positive-strand RNA viruses agronomic medical importance encode superfamily 1 helicases. Although helicases play important roles in viral replication, crystal structures have not been determined. Here, we report structure a fragment (S666 to Q1116) replication protein from virus. reveals novel N-terminal domain tightly associated with helicase core. core contains two RecA-like α/β domains without any accessory insertions that...
Significance The Red Queen hypothesis proposes that host defense genes evolve to counter the adverse effects of rapidly evolving invasive viruses. Although 3D structures host–viral protein complexes have provided great insights into molecular conflicts between them, a single structure represents only an evolutionary snapshot. Here we present atomic details step-by-step arms race tomato mosaic virus replication and inhibitor Tm-1, in which recognition viral molecule, adaptive evasion...
Previously, we isolated a 4‐kDa peptide capable of binding to 43‐kDa receptor‐like protein and stimulating kinase activity the in soybean. Both them were found localize plasma membranes cell walls. Here, report physiological effects expressed transiently cultured carrot bird's‐foot trefoil cells transfected with pBI 121 plasmid containing gene. At early developmental stage, transgenic callus grew rapidly compared wild both species. Cell proliferation vitro nonembryogenic was apparently...
We found that the CutA1 protein, from Pyrococcus horikoshii ( Ph CutA1), has an extremely high denaturation temperature T d ) of nearly 150 °C, which exceeds highest record determined by DSC about 30 °C. To elucidate mechanism ultra‐high stability CutA1, we analyzed crystal structures proteins three different sources, P. , Thermus thermophilus and Escherichia coli with growth temperatures (98, 75, 37 °C). This analysis revealed remarkably increased number ion pairs in monomeric structure...