A5008991478- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Antifungal resistance and susceptibility
- Nanoparticle-Based Drug Delivery
- Advanced Biosensing Techniques and Applications
- Virus-based gene therapy research
- Infectious Diseases and Mycology
- Advanced Polymer Synthesis and Characterization
- Immunotherapy and Immune Responses
- Fungal Infections and Studies
- Hydrogels: synthesis, properties, applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Synthesis and properties of polymers
- Click Chemistry and Applications
- Reproductive System and Pregnancy
- Nanoplatforms for cancer theranostics
- Glycosylation and Glycoproteins Research
- Pneumonia and Respiratory Infections
- Layered Double Hydroxides Synthesis and Applications
- Surfactants and Colloidal Systems
- CAR-T cell therapy research
- DNA and Nucleic Acid Chemistry
- Immune Cell Function and Interaction
- Acute Lymphoblastic Leukemia research
Kyushu University
2016-2025
Kobe Children's Hospital
2020-2025
Aichi Medical University
2000-2023
Kobe University Hospital
2015-2022
Japanese Red Cross Kumamoto Hospital
2019-2022
Juntendo University
2003-2021
Mukogawa Women's University
2021
Hiroshima University
2019-2021
Center for Strategic Research
2021
Kobe University
2014-2020
Gold nanorods can be used as photothermal converters, permitting near-infrared (NIR) light to transmitted deep into tissues without causing damage. We prepared hybrid with a core−shell structure, i.e., single gold nanorod encapsulated in poly (N-isopropylacrylamide) nanogel. Hybrid demonstrated remote, reversible, pulsatile phase transition and vivo action after irradiation using NIR laser.
OBJECTIVE The clinical significance of cytomegalovirus (CMV) reactivation complicating ulcerative colitis (UC) patients has been uncertain. It therefore remained undetermined whether or not CMV should be treated in UC under immunosuppression. aim the study was to clarify natural history patients. METHODS Sixty-nine with moderate severe activity were enrolled study. All prednisolone, and/or immunosuppressants such as cyclosporine A. We sequentially monitored every 2 wk up until 8 using...
By means of a cell culture method, the attachment and growth mouse L929 fibroblast cells were studied on matrices (-SDS)- (+SDS)-keratins, which extracted from wool in absence presence sodium dodecyl sulfate, respectively. The (+SDS)-keratin showed some toxic effect growth, but upon washing with pH 7/phosphate buffer, protein behaved similarly to substratum (-SDS)-keratin. comparative assay keratins, collagen (type I), glass revealed that keratins more adhesive supportive for proliferation...
The efficacy and safety of micafungin (FK463), which is a new lipopeptide antifungal agent the echinocandin class active against both Aspergillus Candida species, were investigated in patients with deep-seated mycosis this study. 70 treated 12.5–150 mg/d intravenously for up to 56 d. overall clinical response rates 60% (6/10) invasive pulmonary aspergillosis, 67% (6/9) chronic necrotizing 55% (12/22) aspergilloma, 100% (6/6) candidemia, 71% (5/7) esophageal candidiasis. prior treatment was...
In this study, we prepared gold nanorod (NR)-embedded N-isopropylacrylamide (NIPAM) hydrogels and studied their volume phase transition behavior induced by near-infrared (near-IR) laser irradiation utilizing the photothermal conversion characteristics of NRs. When poly(ethylene glycol)-modified NRs were used for preparation composite gels, showed marked dispersion stability in gel. Near-IR gel (cylindrical shape, diameter = 140 μm) under following conditions, NR concentrations ≥100 μM power...
BACKGROUND: There are few effective approaches to infertile patients with repeated failure in IVF–embryo transfer therapy. Since recent evidence suggests that some populations of maternal immune cells positively support embryo implantation, we have developed a new approach using peripheral blood mononuclear (PBMCs). METHODS: Patients who had not experienced successful pregnancy despite four or more sessions were enrolled this study (n = 35, 35 cycles). PBMCs obtained from on the day oocyte...
Human luteal cells have been reported to express human leukocyte antigen-DR and lymphocyte functional antigen-3 on the cell surface, suggesting physiological interaction between T-lymphocytes through menstrual cycle into early pregnancy. To elucidate role of peripheral lymphocytes corpus luteum differentiation, effect blood mononuclear (PBMC) steroidogenesis by was investigated. The production Th-2 cytokines such as interleukin (IL)-4 IL-10 co-cultured also examined, effects these...
We isolated three strains of canine distemper virus (CDV)-the Ueno, Hamamatsu, and Yanaka -from dogs in Japan analysed the molecular properties their haemagglutinin (H) proteins. Immunoprecipitation all with a monoclonal antibody revealed H proteins masses 84 kDa, which differs from mass (78 kDa) protein Onderstepoort vaccine strain. However, after tuni- camycin treatment immunoprecipitation identified identical (68 for field isolates Sequence analysis showed nine potential sites...
The thermal properties and temperature-responsive nanoparticle formation of poly(N-isopropylacrylamide) grafted with single-stranded DNA (PNIPAAm-g-DNA) were investigated. Copolymerization between nonamer a vinyl group at its 5' terminus (DNA macromonomer) NIPAAm was carried out so that the macromonomer unit content should be less than 1 mol %. turbidimetry differential scanning calorimetry copolymer showed transition temperature increased enthalpy change phase decreased increasing in...
Abstract Toward the development of a novel cancer immunotherapy, we have previously identified several tumor‐associated antigens (TAAs) and epitopes recognized by human histocompatibility leukocyte (HLA)‐A2/A24‐restricted cytotoxic T lymphocyte (CTL). In this study, tried to identify TAA lung (LC) its HLA‐A2 restricted CTL provide target antigen useful for immunotherapy LC. We testis antigen, cell division cycle associated gene 1 (CDCA1), overexpressed in nonsmall LC using cDNA microarray...
Abstract Gold nanorods, which have a strong surface plasmon band at the near-infrared region, absorb light energy is then converted to heat. Since can penetrate deeply into tissue, gold nanorods are expected be useful as photosensitizers for photothermal therapy. In this study, length of poly(ethylene glycol) (PEG) chain was optimized in order stabilize blood circulation after intravenous injection. PEG5000- and PEG10000-modified showed higher stability compared with PEG2000-...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDesign of Polymeric Carriers for Cancer-Specific Gene Targeting: Utilization Abnormal Protein Kinase Cα Activation in Cancer CellsJeong-Hun Kang*†‡, Daisuke Asai§, Jong-Hwan Kim∥, Takeshi Mori†⊥, Riki Toita†, Tetsuro Tomiyama†, Yoji Asami†, Jun Oishi†, Yuko T. Sato†‡, Takuro Niidome†⊥, Byungdug Jun∥, Hideki Nakashima‡§, and Yoshiki Katayama*†‡⊥View Author Information Department Applied Chemistry, Faculty Engineering, Kyushu University, 744...
Intravenous delivery of apolipoprotein B-specific siRNA with a sixth-generation dendritic poly(L-lysine) (KG6) resulted in siRNA-mediated knockdown ApoB healthy C57BL/6 mice without hepatotoxicity, and significant reduction serum low-density lipoprotein cholesterol E-deficient mice.
Controlled-release systems that respond to external stimuli have received great interest for use in medical treatments such as drug delivery specific sites. Gold nanorods an absorption band at the near-infrared region and convert absorbed light energy into heat, which is known a "photothermal effect". Therefore, gold are expected act not only on-demand thermal converter photothermal therapy but also controller of drug-release system capable responding irradiation. In this study, construct...
We propose a method to improve the enhanced permeability and retention (EPR) effect of nanomedicines based on tumor-specific vasodilation using nitric oxide (NO) donor-containing liposome. NONOate, typical NO donor, was incorporated into PEGylated liposome retard protonation-induced release from NONOate by protecting lipid bilayer membrane. The NONOate-containing (NONOate-LP) showed similar blood an empty but almost twice amount accumulated within tumor. This improvement in EPR is thought...