A5100680389- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Renal Diseases and Glomerulopathies
- Synthetic Organic Chemistry Methods
- Advanced MRI Techniques and Applications
- Advanced X-ray Imaging Techniques
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Medical Imaging Techniques and Applications
- Asymmetric Synthesis and Catalysis
- Cardiac Imaging and Diagnostics
- Oxidative Organic Chemistry Reactions
- Advanced X-ray and CT Imaging
- Chemical Synthesis and Reactions
- Chemical Synthesis and Analysis
- Drug Transport and Resistance Mechanisms
- Chronic Kidney Disease and Diabetes
- Alzheimer's disease research and treatments
- Cell Adhesion Molecules Research
- Synthesis of heterocyclic compounds
- Carbohydrate Chemistry and Synthesis
- Genetics, Aging, and Longevity in Model Organisms
- Asymmetric Hydrogenation and Catalysis
- Vasculitis and related conditions
- Birth, Development, and Health
- Acute Lymphoblastic Leukemia research
Kitasato University
2009-2024
Dokkyo Medical University
2012-2023
Social Insurance Saitama Chuo Hospital
2018-2023
Dokkyo Medical University Saitama Medical Center
2013-2022
Wakayama University
2015-2022
Kindai University
2020-2022
Weatherford College
2021
Cardiff University
2020
Osaka University
1973-2018
Koto Hospital
2018
Podocalyxin (PC), the major sialoprotein of glomerular epithelial cells (GECs), helps maintain characteristic architecture foot processes and patency filtration slits. PC associates with actin via ezrin, a member ERM family cytoskeletal linker proteins. Here we show that is linked to ezrin cytoskeleton Na+/H+-exchanger regulatory factor 2 (NHERF2), scaffold protein containing two PDZ (PSD-95/Dlg/ZO-1) domains an ERM-binding region. The cytoplasmic tail contains C-terminal PDZ-binding motif...
Podocalyxin is a major membrane protein of the glomerular epithelium and thought to be involved in maintenance architecture foot processes filtration slits characteristic this unique by virtue its high negative charge. However, until now there has been no direct evidence for podocalyxin's function. type 1 transmembrane sialoprotein with an N-terminal mucin-like domain. To assess function, we cloned rat podocalyxin examined effects expression on cell adhesion properties stably transfected...
Abstract. During development, renal glomerular epithelial cells (podocytes) undergo extensive morphologic changes necessary for creation of the filtration apparatus. These include formation interdigitating foot processes, replacement tight junctions with slit diaphragms, and concomitant opening intercellular urinary spaces. It was postulated previously confirmed recently that podocalyxin, a sialomucin, plays major role in maintaining space open by virtue physicochemical properties its highly...
ABSTRACT. Podocalyxin (PC) is the major sialoglycoprotein expressed on apical membrane of podocyte. Previously it was shown that PC connected to actin through PC/NHERF2/ezrin complex, and this connection disrupted in nephrotic syndrome. For assessing whether expression affects organization cytoskeleton, MDCK cell lines stably expressing either full-length or a mutant lacking NHERF binding site established. It found but not actin, induces redistribution toward membrane, leads increased RhoA...
The success of pancreatic β-cells transplantation to treat type 1 diabetes has been hindered by massive β-cell dysfunction and loss that follows the procedure. Hypoxia-mediated cell death considered one main difficulties must be overcome for regarded as a reliable therapy. Here we have investigated mechanisms underlying in response hypoxia (1% O2). Our studies show mouse insulinoma line 6 (Min6) cells undergo apoptosis with caspase-3 activation occurring early 2 h following exposure hypoxia....
Leptin is secreted by adipocytes and a circulating factor that regulates food intake energy expenditure. Its serum level elevated in patients with renal failure has been suggested to be associated malnutritional factors these patients. primarily metabolized the kidneys, although precise molecular mechanisms are not known. The purpose of this study was determine nephron segments potential receptors involved leptin metabolism. To segment uptake, we performed histoautoradiography kidney...
Megalin is an endocytic receptor that binds multiple ligands and essential for many physiological processes such as brain development uptake of proteins by the kidney tubule, yolk sac, thyroid. The cytoplasmic tail megalin contains two FXNPXY motifs. Autosomal recessive hypercholesterolemia (ARH) adaptor protein to motif low-density lipoprotein well clathrin AP-2. We found ARH also first in two-hybrid, pull-down coimmunoprecipitation assays. colocalizes with coated pits recycling endosomes...
Two putative serum precursors which cross-react with antiserum against murine senile amyloid protein (ASSAM) were isolated from the high density lipoprotein (HDL) of normal mouse serum.Apolipoproteins designated "aPOSASsA~-I" and POSAS SASS AM-^" have same molecular weight as tissue fibril protein.ApoSASSAM-1 apoSASsAM.2migrate to an intermediate position between apoA-I apoC on alkalineurea polyacrylamide gel electrophoresis are present mainly in HDL apoproteins a slight extent very low when...
Three types of apolipoprotein A-II (apoA-II) proteins (A, B and C) were predicted from the nucleotide sequence apoA-II cDNA. Substitution amino acid residues was noted at four positions (type A: Pro-5, Asp-20, Met-26, Ala-38; B: Glu-20, Val-26, Val-38; C: Gln-5, Ala-38). Each type identifiable by digestion amplified DNA PCR, using restriction-fragment-length polymorphism gene for restriction enzymes Cfr13I MspI. The molecular determined among 23 strains mice including nine senescence...
We studied 96 infants and children with untreated neuroblastomas. Chromosomes of tumor cells were analyzed in 68, N-myc copy numbers determined 67 patients. Patients found by a mass screening program for 6-month-old (group A1, 39 patients) or those less than 12 months age clinically A2, 13 rarely the disseminated stage (A1, three 39; A2 one 13); their tumors usually had near-triploid (3n) hypertetraploid (greater 4n) karyotypes 28 37; nine 11), never amplification zero 34; 11). In contrast,...
Impairment of proximal tubular endocytosis glomerular-filtered proteins including albumin results in the development proteinuria/albuminuria patients with chronic kidney disease. However, mechanisms regulating function are largely unknown. This study aimed to investigate role angiotensin II type 1A receptor (AT(1A)R)- and insulin-mediated signaling pathways expression megalin, a multiligand endocytic tubule cells (PTCs). Opossum PTC-derived OK that stably express rat AT(1A)R but deficient...
OBJECTIVE Megalin, an endocytic receptor in proximal tubule cells, is involved the mechanisms of albuminuria diabetic nephropathy (DN). To develop efficient novel biomarkers associated with pathogenesis DN, we investigated urinary megalin excretion type 2 diabetes. RESEARCH DESIGN AND METHODS Sandwich enzyme-linked immunosorbent assay systems were established monoclonal antibodies against NH2 (amino [A]-megalin assay) and COOH (C-megalin termini to analyze forms 68 patients RESULTS The...
We describe a new attenuation correction method for fluorescent X-ray computed tomography (FXCT) applied to image nonradioactive contrast materials in vivo. The principle of the FXCT imaging is that first generation. Using monochromatized synchrotron radiation from BLNE-5A bending-magnet beam line Tristan Accumulation Ring KEK, Japan, we studied phantoms with method, and succeeded delineating 4-mm-diameter channel filled 500 /spl mu/g I/ml iodine solution 20-mm-diameter acrylic cylindrical...