A5103036778- Kawasaki Disease and Coronary Complications
- Blood Coagulation and Thrombosis Mechanisms
- Coronary Artery Anomalies
- Streptococcal Infections and Treatments
- Coronary Interventions and Diagnostics
- Angiogenesis and VEGF in Cancer
- Inflammasome and immune disorders
- Mechanical Circulatory Support Devices
- Neonatal and Maternal Infections
- RNA Interference and Gene Delivery
- Peripheral Artery Disease Management
- Virus-based gene therapy research
- Cardiac Imaging and Diagnostics
- Venous Thromboembolism Diagnosis and Management
- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Erythrocyte Function and Pathophysiology
- Cardiovascular Issues in Pregnancy
- Liver Disease Diagnosis and Treatment
- Vasculitis and related conditions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bone Metabolism and Diseases
- Muscle Physiology and Disorders
- Acute Kidney Injury Research
- Xenotransplantation and immune response
Nagasaki Medical Center
2019-2022
National Defense Medical College
2002-2020
National Defense Medical College Hospital
1997-2015
Nagasaki Municipal Hospital
2013
Ibaraki University
2005-2011
Gunma Children's Medical Center
2011
Gunma University
2011
National Institute of Technology and Evaluation
2007
Nagasaki University
1992-2007
Tohoku University
2007
Vascular endothelial growth factor (VEGF) is a heparin-binding, cell-specific mitogen. Previous studies have suggested that VEGF regulator of naturally occurring physiologic and pathologic angiogenesis. In this study we investigated the hypothesis angiogenic potential sufficient to constitute therapeutic effect. The soluble 165-amino acid isoform was administered as single intra-arterial bolus internal iliac artery rabbits in which ipsilateral femoral excised induce severe, unilateral hind...
A cDNA library prepared from the mouse osteoblastic cell line MC3T3-E1 was screened for presence of specifically expressed genes by employing a combined subtraction hybridization/differential screening approach. identified and sequenced which encodes protein designated osteoblast-specific factor 2 (OSF-2) comprising 811 amino acids. OSF-2 has typical signal sequence, followed cysteine-rich domain, fourfold repeated domain C-terminal domain. The lacks transmembrane region. shows homology with...
I"
This study was designed to assess the physiological consequences of augmented vascularity induced by administration vascular endothelial growth factor (VEGF), an cell-specific mitogen, in a rabbit model hindlimb ischemia. Ten days after excision common and superficial femoral arteries from one 24 New Zealand White rabbits, VEGF (n = 15) or saline (control; n 9) selectively injected into ipsilateral internal iliac artery. Limb perfusion evaluated immediately pre-VEGF (baseline) again at 10...
The possibility of using an exclusively percutaneous strategy to deliver foreign DNA normal and balloon-dilated atherosclerotic arteries was studied by analysis transfection efficiency in a rabbit model. A total 22 external iliac from rabbits (10 12 atherosclerotic) were transfected with solution luciferase expression vector plasmid liposome, dual balloon-catheter system. Analysis the segments revealed activity 10 (4/10 vs 6/12 balloon-injured atherosclerotic, P = NS); no could be detected...
Markedly activated neutrophils or higher plasma levels of neutrophil elastase are involved in the poor response to intravenous immunoglobulin (IVIG) and formation coronary artery lesions (CAL) patients with acute Kawasaki disease. We hypothesized that ulinastatin (UTI), by both direct indirect suppression neutrophils, would reduce occurrence CAL.We retrospectively analyzed clinical records disease between 1998 2009. Three hundred sixty-nine were treated a combination UTI, aspirin, IVIG as an...
Arterial gene transfer represents a novel strategy that is potentially applicable to variety of cardiovascular disorders. Attempts perform arterial using nonviral vectors have been compromised by low transfection efficiency. We investigated the hypothesis cellular proliferation induced injury could augment expression after liposome-mediated transfer. Nondenuded and denuded rabbit strips were maintained in culture for up 21 d, which was performed with mixture plasmid encoding firefly...
Background We recently reported that the combination of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte (PLR) is a novel useful predictor intravenous immunoglobulin (IVIG)-resistance in Kawasaki disease (KD). In present study, to evaluate effectiveness new risk score, we compared its predictive validity previously scores. Materials methods The laboratory records 437 patients with KD before IVIG therapy were retrospectively analyzed, IVIG-responsive (n = 344) IVIG-resistant...
To investigate the inhibitory effect of serine protease inhibitors (SPI) on neutrophil-mediated endothelial cell (EC) injury, we analyzed in vitro cytotoxicity radiolabeled human umbilical vein EC (HUVEC) mediated by neutrophils presence SPI. The injury was inhibited dose-dependently urinary trypsin inhibitor (ulinastatin, UTI) and ONO-5046, which have ability to inactivate neutrophil elastase, but not gabexate mesilate, nafamostat aprotinin, argatroban, no elastase. In addition, when UTI...
summary Recent reports have demonstrated that circulating endothelial cells (CECs) are observed in several diseases with vascular injury. Because Kawasaki disease (KD) is one type of systemic vasculitis, we hypothesized an increased number CECs may be associated the appearance complicated coronary artery lesions (CAL). In present study investigated enumeration and origin 20 patients KD, using immunohistochemical method monoclonal antibodies: clone P1H12 against ECs AC133 progenitor (EPCs),...
BACKGROUND The development of molecular strategies for the treatment restenosis has been hindered by low efficiencies in vivo arterial transfection. Expression intracellular marker proteins is generally evident < 1% vascular smooth muscle cells after Efforts to improve efficiency gene transfer have further impeded use transgenes encoding proteins, necessitating tissue removal and limiting survey expression one point time. METHODS AND RESULTS To study on a serial basis determine relation...
Matrix metalloproteinases (MMPs) play an important role in the progression of tumour cells and invasion inflammatory by degrading extracellular matrix. In MMP family, MMP-9 gelatinase is thought to contribute pathogenesis arteritis disrupting elastic lamina. The aim present study investigate potential Kawasaki disease (KD), acute type systemic vasculitis children. We studied total levels (free proMMP-9 free MMP-9) sera using a new assay system expression mRNA leucocytes reverse...
BACKGROUND Complexing recombinant DNA with cationic liposomes is a convenient means of introducing foreign genes into cells (lipofection) and could potentially form the basis for genetically modifying diseased blood vessels in patients. The mechanism lipofection incompletely understood, but it recognized that degree successful gene transfer highly dependent on cell type. To date, there has been no reported experience human vascular smooth muscle cells. METHODS AND RESULTS Primary cultures...
Objectives: Natural angiogenesis has been shown to be impaired in spontaneously hypertensive rats (SHR). The purpose of this study was determine whether pathological the setting tissue ischemia is also SHR, and what extent it modified by angiotensin-converting enzyme (ACE) inhibition. Methods: Ischemia induced hindlimb SHR excision femoral artery, after which animals were randomly assigned receive low-dose perindopril (sub-antihypertensive, 0.2 mg/kg/day), high-dose (antihypertensive, 2.0 or...