A5079342872- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Zebrafish Biomedical Research Applications
- Cancer, Hypoxia, and Metabolism
- Lymphatic System and Diseases
- Electrospun Nanofibers in Biomedical Applications
- Peripheral Artery Disease Management
- Coronary Interventions and Diagnostics
- Cardiac and Coronary Surgery Techniques
- Cerebrovascular and Carotid Artery Diseases
- Pluripotent Stem Cells Research
- Cardiac Fibrosis and Remodeling
- Hedgehog Signaling Pathway Studies
- MicroRNA in disease regulation
- Biomarkers in Disease Mechanisms
- Extracellular vesicles in disease
- Immune cells in cancer
- Bone fractures and treatments
- Axon Guidance and Neuronal Signaling
- Photodynamic Therapy Research Studies
- Venous Thromboembolism Diagnosis and Management
- Nitric Oxide and Endothelin Effects
- Pregnancy and preeclampsia studies
Tokai University
2014-2024
Shonan Kamakura General Hospital
2021-2024
Tokai University Hospital
2008-2020
Nagasaki University
2019
Foundation for Biomedical Research and Innovation
2007-2018
RIKEN Center for Computational Science
2006-2016
Ajinomoto (Japan)
2016
Institute for Biomedical Research and Innovation
2010-2013
University of Yamanashi
1999-2013
Pusan National University
2011-2013
Putative endothelial cell (EC) progenitors or angioblasts were isolated from human peripheral blood by magnetic bead selection on the basis of surface antigen expression. In vitro, these cells differentiated into ECs. animal models ischemia, heterologous, homologous, and autologous EC incorporated sites active angiogenesis. These findings suggest that may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) delivering anti- pro-angiogenic agents,...
Abstract —Circulating endothelial progenitor cells (EPCs) have been isolated in peripheral blood of adult species. To determine the origin and role EPCs contributing to postnatal vasculogenesis, transgenic mice constitutively expressing β-galactosidase under transcriptional regulation an cell–specific promoter (Flk-1/LZ or Tie-2/LZ) were used as transplant donors. Localization EPCs, indicated by flk-1 tie-2/lacZ fusion transcripts, identified corpus luteal endometrial neovasculature after...
Animal studies and preliminary results in humans suggest that lower extremity myocardial ischemia can be attenuated by treatment with angiogenic cytokines. The resident population of endothelial cells is competent to respond an available level growth factors, however, may potentially limit the extent which cytokine supplementation enhances tissue neovascularization. Accordingly, we transplanted human progenitor (hEPCs) athymic nude mice hindlimb ischemia. Blood flow recovery capillary...
Background —We investigated the therapeutic potential of ex vivo expanded endothelial progenitor cells (EPCs) for myocardial neovascularization. Methods and Results —Peripheral blood mononuclear obtained from healthy human adults were cultured in EPC medium harvested 7 days later. Myocardial ischemia was induced by ligating left anterior descending coronary artery male Hsd:RH-rnu (athymic nude) rats. A total 10 6 EPCs labeled with 1,1′-dioctadecyl-1 to 3,3,3′,3′-tetramethylindocarbocyanine...
We tested the hypothesis that endothelial nitric oxide synthase (eNOS) modulates angiogenesis in two animal models which therapeutic has been characterized as a compensatory response to tissue ischemia. first administered L-arginine, previously shown augment endogenous production of NO, normal rabbits with operatively induced hindlimb Angiogenesis ischemic was significantly improved by dietary supplementation compared placebo-treated controls; angiographically evident vascularity limb,...
Stromal cell-derived factor-1 (SDF-1) is a chemokine considered to play an important role in the trafficking of hematopoietic stem cells. Given close relationship between cells and endothelial progenitor (EPCs), we investigated effect SDF-1 on EPC-mediated vasculogenesis.Flow cytometric analysis demonstrated expression CXCR4, receptor SDF-1, by 66+/-3% EPCs after 7 days culture. In vitro modified Boyden chamber assay showed dose-dependent EPC migration toward (control versus 10 ng/mL 100...
Background — Primary and secondary prevention trials suggest that statins possess favorable effects independent of cholesterol reduction. We investigated whether statin therapy may also accelerate reendothelialization after carotid balloon injury. Methods Results Simvastatin treatment in 34 male Sprague-Dawley rats accelerated the balloon-injured arterial segments (reendothelialized area at 2 weeks, 12.3±1.8 versus 5.4±1.1 mm , P < 0.01) resulted a dose-dependent (0.2 or 1 mg/kg IP)...
The effect of aging on angiogenesis in ischemic vascular disease has not been studied. Accordingly, we investigated the hypothesis that is impaired as a function age.Forty days after resection 1 femoral artery, collateral vessel development was significantly old (aged 4 to 5 years; n=7) versus young 6 8 months; n=6) New Zealand White (NZW) rabbits basis reduced hindlimb perfusion (ischemic: normal blood pressure ratio=0.58+/-0.05 0.77+/-0.06; P<0.005), number angiographically visible vessels...
Abstract —Angiopoietin-1 (Ang1) has been recently identified as the major physiological ligand for tyrosine kinase receptor Tie2 and assigned responsibility recruiting sustaining periendothelial support cells. Angiopoietin-2 (Ang2) was found to disrupt blood vessel formation in developing embryo by antagonizing effects of Ang1 thus considered represent a natural Ang1/Tie2 inhibitor. In vivo either angiopoietin on postnatal neovascularization, however, have not previously described....
Background— We investigated whether catheter-based, intramyocardial transplantation of autologous endothelial progenitor cells can enhance neovascularization in myocardial ischemia. Methods and Results— Myocardial ischemia was induced by placement an ameroid constrictor around swine left circumflex artery. Four weeks after placement, CD31+ mononuclear (MNCs) were freshly isolated from the peripheral blood each animal. After overnight incubation MNCs noncoated plates, nonadhesive (NA/CD31+...
Endothelial progenitor cells (EPCs) have been isolated from circulating mononuclear in peripheral blood and shown to incorporate into foci of neovascularization, consistent with postnatal vasculogenesis. These EPCs are derived bone marrow mobilized endogenously response tissue ischemia or exogenously by cytokine stimulation. We show here, using a chemotaxis assay vitro EPC culture simvastatin-treated animals vivo, that the HMG-CoA reductase inhibitor, simvastatin, augments population EPCs....
Background Recent studies have suggested that vascular endothelial growth factor (VEGF) and basic fibroblast (bFGF) may synergistic effects on the induction of angiogenesis in vitro. Therefore, we investigated hypothesis simultaneous administration VEGF bFGF, each having been previously shown to independently enhance collateral development an animal model hind limb ischemia, could a effect vivo. Methods Results Ten days after surgical unilateral New Zealand White rabbits were randomized...
Endothelial progenitor cells (EPCs) have been isolated from circulating mononuclear in peripheral blood and shown to incorporate into foci of neovascularization, consistent with postnatal vasculogenesis. These EPCs are derived bone marrow mobilized endogenously response tissue ischemia or exogenously by cytokine stimulation. We show here, using a chemotaxis assay vitro EPC culture simvastatin-treated animals vivo, that the HMG-CoA reductase inhibitor, simvastatin, augments population EPCs....
Previous studies have established that bone marrow-derived endothelial progenitor cells (EPCs) are present in the systemic circulation. In current study, we investigated hypothesis gene transfer can be used to achieve phenotypic modulation of EPCs.In vitro, ex vivo murine vascular growth factor (VEGF) 164 augmented EPC proliferative activity and enhanced adhesion incorporation EPCs into quiescent as well activated cell monolayers. To determine if such may facilitate therapeutic...
Preclinical studies in animal models and early results of clinical trials patients suggest that intramuscular injection naked plasmid DNA encoding vascular endothelial growth factor (VEGF) can promote neovascularization ischemic tissues. Such has been attributed exclusively to sprout formation cells derived from preexisting vessels. We investigated the hypothesis VEGF gene transfer may also augment population circulating progenitor (EPCs). In with critical limb ischemia receiving transfer,...
Background Most strategies designed to reduce restenosis by the use of pharmacological or biological reagents involve direct inhibition vascular smooth muscle cell (SMC) proliferation. Alternatively, SMC proliferation might be indirectly inhibited if reendothelialization could specifically facilitated at sites balloon-induced arterial injury. Accordingly, we investigated hypothesis that application an endothelial (EC)-specific mitogen a freshly denuded intimal surface accelerate and thereby...
Background— A growing population of patients with coronary artery disease experiences angina that is not amenable to revascularization and refractory medical therapy. Preclinical studies have indicated human CD34 + stem cells induce neovascularization in ischemic myocardium, which enhances perfusion function. Methods Results— Twenty-four (19 men 5 women aged 48 84 years) Canadian Cardiovascular Society class 3 or 4 who were undergoing optimal treatment candidates for mechanical enrolled a...
We have identified a subpopulation of stem cells within adult human BM, isolated at the single-cell level, that self-renew without loss multipotency for more than 140 population doublings and exhibit capacity differentiation into all 3 germ layers. Based on surface marker expression, these clonally expanded BM-derived multipotent (hBMSCs) do not appear to belong any previously described cell population. Intramyocardial transplantation hBMSCs after myocardial infarction resulted in robust...