A5026843792- Antibiotics Pharmacokinetics and Efficacy
- Inflammatory mediators and NSAID effects
- Pharmacological Effects and Toxicity Studies
- Metabolism and Genetic Disorders
- Antiplatelet Therapy and Cardiovascular Diseases
- Coronary Interventions and Diagnostics
- Electrolyte and hormonal disorders
- Drug Transport and Resistance Mechanisms
- Immune Response and Inflammation
- Synthesis of β-Lactam Compounds
- Pharmaceutical studies and practices
- Blood disorders and treatments
- Asthma and respiratory diseases
- Renal function and acid-base balance
- Drug-Induced Hepatotoxicity and Protection
- Angiogenesis and VEGF in Cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Atherosclerosis and Cardiovascular Diseases
- Eicosanoids and Hypertension Pharmacology
- Adenosine and Purinergic Signaling
- Apelin-related biomedical research
- Protease and Inhibitor Mechanisms
- Medical and Biological Ozone Research
- S100 Proteins and Annexins
- Diet and metabolism studies
Inserm
2007-2018
Sorbonne Paris Cité
2018
Laboratoire de Recherche Vasculaire Translationnelle
2015-2017
Hôpital Bichat-Claude-Bernard
2015
University of Geneva
1966-2015
Université de Rouen Normandie
2015
Institut de génétique et de biologie moléculaire et cellulaire
2007-2013
Université de Strasbourg
2012
Centre National de la Recherche Scientifique
2012
Assistance Publique – Hôpitaux de Paris
2010
The effect of aging on angiogenesis in ischemic vascular disease has not been studied. Accordingly, we investigated the hypothesis that is impaired as a function age.Forty days after resection 1 femoral artery, collateral vessel development was significantly old (aged 4 to 5 years; n=7) versus young 6 8 months; n=6) New Zealand White (NZW) rabbits basis reduced hindlimb perfusion (ischemic: normal blood pressure ratio=0.58+/-0.05 0.77+/-0.06; P<0.005), number angiographically visible vessels...
Prostanoids, bioactive lipids derived from arachidonic acid (AA), are important for vascular homeostasis. Among them, prostaglandin E2 (PGE2) enhances aggregation of platelets submaximally stimulated in vitro. This results activation EP3, one the four PGE2 receptors, which decreases threshold at agonists activate to aggregate. Although altered venous thrombosis induced by administration AA, its role pathophysiopathological conditions has remained speculative. We report that arterial walls...
Background —Endothelial cells (ECs) represent the critical cellular element responsible for postnatal angiogenesis. Because ACE inhibitors may favorably affect endothelial function, we investigated hypothesis that administration of inhibitor quinaprilat could enhance angiogenesis in vivo. Methods and Results —Ten days after resection 1 femoral artery, New Zealand White (NZW) rabbits were randomly assigned to receive recombinant human vascular growth factor (rhVEGF) administered as a single...
The importance of arachidonic acid metabolites (termed eicosanoids), particularly those derived from the COX-1 and COX-2 pathways prostanoids), in platelet homeostasis has long been recognized. Thromboxane is a potent agonist, whereas prostacyclin an inhibitor aggregation. In contrast, effect prostaglandin E2 (PGE2) on aggregation varies significantly depending its concentration. Low concentrations PGE2 enhance aggregation, high levels inhibit mechanism for this dual action not clear. This...
Background: The great variability of periodontal and systemic responses to experimental periodontitis reflects the inherent pathogenic complexity mice models could limit resulting interpretations their extension human diseases. This study compared effect Porphyromonas gingivalis ( Pg ) infection duration at local levels in various models. Methods: Periodontitis was induced C57BL/6J by ligatures previously incubated with (LIGPG group) or not (LIG oral gavage (GAV) ATCC 33277. Blood samples...
AimsHaemostasis interrupts bleeding from disrupted blood vessels by activating platelet aggregation and coagulation. A similar mechanism termed thrombosis generates obstructive thrombi inside diseased arteries. As a consequence of this similarity, current anti-thrombotic agents increase the risk bleeding. Atherosclerotic plaques produce significant amounts prostaglandin E2 (PGE2), which activates its receptor EP3 on platelets aggravates atherothrombosis. We investigated whether blocking...
Both leukotrienes and neutrophils have been linked to plaque destabilization. Despite being evoked, the role of leukotriene B4 (LTB4) in neutrophil recruitment plaques concomitant effects these two actors on stability remain be proven. Since both are elicited during endotoxaemia, a condition associated with risk cardiovascular events, we investigated whether endotoxaemia promotes LTB4-mediated infiltration explored roles LTB4 Endotoxaemia induced by repeated peritoneal endotoxin injections...
Objective— Atherosclerotic plaque rupture leads to acute thrombus formation and may trigger serious clinical events such as myocardial infarction or stroke. Therefore, it would be valuable identify atherothrombosis vulnerable plaques before the onset of events. We sought determine whether noninvasive in vivo visualization activated platelets was effective when using a target-specific MRI contrast agent thrombi, hallmarks high-risk atherosclerotic plaques. Methods Results— Inflammatory...
Atherosclerotic plaque phenotypes are classified based on the extent of macrophage infiltration into lesions, and presence certain subsets might be a sign for vulnerability. The mannose receptor (MR) is over-expressed in activated macrophages. Tilmanocept tracer that targets MR approved Europe USA detection sentinel lymph nodes. In this study, our aim was to evaluate potential 111In-labelled tilmanocept MR-positive macrophages atherosclerotic plaques apolipoprotein E-knockout (ApoE-KO) mouse...
Leukotrienes are lipid mediators that evoke primarily proinflammatory responses by activating receptors present on virtually all cells. The production of leukotrienes is tightly regulated, and expression 5-lipoxygenase, the enzyme required for first step in leukotriene synthesis, generally restricted to leukocytes. Arachidonic acid released from cell membrane activated leukocytes rapidly converted LTA4 5-lipoxygenase. further metabolized either LTC4 or LTB4 synthase hydrolase, respectively....
Purinergic receptors are expressed throughout the respiratory system in diverse cell types. The efficiency of mucus clearance airways, cascade leading to tissue injury, and inflammation modulated by autocrine/paracrine release nucleotides signaling purinergic receptors. We assessed role innate host defense lung vivo infecting mice deficient P2Y1, P2Y2, or both with intratracheal instillation Pseudomonas aeruginosa. After P. aeruginosa challenge, all double knockout (P2Y1/P2Y2-/-) succumbed...
Little is known about the behaviour of antibiotics in tissues. To investigate this problem, rats were sacrificed groups six, 1, 2, 4, 6, 8, 12 and 18 h after an intraperitoneal injection 10 mg doxycycline/kg body weight. The antibiotic levels determined nine major organs serum by a microbiological method. One hour injection, doxycycline concentrations all tissues already higher than concentrations. After 4 h, concentration exceeded 2.5 mug/ml lungs, muscles, testes heart, much greater...
Background Early and non-invasive detection of platelets on micro atherothrombosis provides a means to identify unstable plaque thereby allowing prophylactic treatment towards prevention stroke or myocardial infarction. Molecular magnetic resonance imaging (mMRI) activated as early markers rupture using targeted contrast agents is promising strategy. In this study, we aim specifically image in murine by vivo mMRI, dedicated animal model rupture. Methods An antibody targeting ligand-induced...
Nephrotoxicity of carboplatin is rare, especially in children with normal renal function. A 3-year-old boy had localized esthesioneuroblastoma and received 2 courses (200 mg/m2/day during a 1-hour infusion for 3 consecutive days) associated etoposide (150 after carboplatin). Because good tumor response, second course was given 21 days later. Complete surgical excision local irradiation were performed. However, the recurred few months later, patient subsequently died disease.Renal function...