A5027091598- Migraine and Headache Studies
- Medical and Biological Ozone Research
- Sympathectomy and Hyperhidrosis Treatments
- Cholesterol and Lipid Metabolism
- Trigeminal Neuralgia and Treatments
- Neurology and Historical Studies
- Cardiovascular Syncope and Autonomic Disorders
- Steroid Chemistry and Biochemistry
- Hedgehog Signaling Pathway Studies
- Neurological Complications and Syndromes
- Pain Mechanisms and Treatments
- Glycogen Storage Diseases and Myoclonus
- Olfactory and Sensory Function Studies
- Pain Management and Placebo Effect
- Pituitary Gland Disorders and Treatments
- Obsessive-Compulsive Spectrum Disorders
- Plant biochemistry and biosynthesis
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Neurosurgical Procedures and Complications
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Pharmaceutical industry and healthcare
- Neuropeptides and Animal Physiology
- Immune Cell Function and Interaction
- Microbial Metabolic Engineering and Bioproduction
University of Liverpool
2023
American Headache Society
2019-2020
New York Proton Center
2020
Rush University
2020
Sleep Research Society
2020
Hartpury University
2018-2019
Carolina Headache Institute
2019
University of Minnesota Medical Center
2013-2018
University of Minnesota
2004-2017
Grant Medical Center
2017
During hedgehog biosynthesis, autocatalytic processing produces a lipid-modified amino-terminal fragment (residues 24–197 in the human Sonic sequence) that is responsible for all known signaling activity and highly conserved evolutionarily. Published <i>in vitro</i> biochemical studies using <i>Drosophila</i> identified membrane anchor as cholesterol, localized site of attachment to COOH terminus fragment. We have expressed full-length insect mammalian cells determined by mass spectrometry...
Support for the role of a cytosolic oxysterol-binding protein in regulation 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was obtained by correlating relative binding affinities wide range oxysterols to their potency suppressing HMG-CoA activity mouse fibroblast cell cultures.Forty-seven encompassing 100- fold both assays were tested and two parameters closely correlated 35 sterols.Twelve sterols showed poor when compared ability suppress cultures.Among these seven with ketone...
Objective.–To assess the scaling properties, reliability, and validity of revised Migraine‐Specific Quality Life Questionnaire (MSQ) (Version 2.1) Background.–The MSQ is a disease‐specific, quality‐of‐life instrument with three hypothesized scales that has been developed, tested, revised. Methods.–The study used multicenter, nondrug, prospective, parallel group, quasi‐experimental design. Patients migraine were recruited at outpatient headache specialty practices administered MSQ, Medical...
Type I interferons (IFN-I) are implicated in the pathogenesis of systemic lupus erythematosus (SLE). In SLE, immune complexes bind to CD32a (FcγRIIa) receptor on surface plasmacytoid dendritic cells (pDCs) and stimulate secretion IFN-I from pDCs. BDCA2 is a pDC-specific that, when engaged, inhibits production human engagement, therefore, represents an attractive therapeutic target for inhibiting pDC-derived may be effective therapy treatment SLE. this study, we show that 24F4A, humanized...
Post-translational modifications of the developmental signaling protein Sonic hedgehog (Shh) by a long-chain fatty acid at N-terminus and cholesterol C-terminus greatly activate in cell-based assay. To investigate structural determinants this activation phenomenon, hydrophobic hydrophilic moieties have been introduced chemical mutagenic methods to soluble N-terminal domain Shh tested both vitro vivo assays. A wide variety increased potency when added protein, ranging from acids amino acids,...
The zona pellucida of mouse oocytes becomes resistant to chymotrypsin digestion, or "hardened", when spontaneous maturation occurs in serum-free medium (De Felici and Siracusa, Gam Res 1982; 6:107). hardened is refractory sperm penetration, thus preventing fertilization. Conversion the glycoprotein ZP2 ZP2f by a protease from precociously released oocyte cortical granules appears be major contributory factor hardening (Ducibella et al., Dev Biol 1990; 137:46). Fetal bovine serum (FBS)...
cholesterol biosynthesis in SI,
Bios~thetically tritiated sterols from Chinese hamster lung (Dede) cells were fractionated by high performance liquid chromatography, and fractions assayed for their ability to repress 3-hydroxy-3-methylglutaryl-CoA reductase in L cell cultures.Most o f the activity found was associated with two oxysterols, 24(~,25-epoxycholesterol 25-hydroxycholesterol.The identities of established eo-chromatography authentic samples isotopic dilution recrystallization.Only low levels repressor other sterol...
Previous studies of peripheral immune cells have documented that activation adenosine 2A receptors (A Rs) decrease proinflammatory cytokine release and increase the potent anti-inflammatory cytokine, interleukin-10 (IL-10). Given growing literature supporting glial cytokines importantly contribute to neuropathic pain IL-10 can suppress such pain, we evaluated effects intrathecally administered A R agonists on using chronic constriction injury (CCI) model. single intrathecal injection...
The interconversion of free and esterified sterols was followed radioisotopically with [U-14C]acetate [methyl-14C]methionine. In pulse-chase experiments, radioactivity first appeared mainly in unesterified exponential-phase cells. Within one generation time, the label equilibrated between sterol pools subsequently accumulated steryl esters stationary-phase When were prelabeled by growing cells aerobically to stationary phase diluted into unlabeled medium, returned form under several...
We have defined regions in the Sonic hedgehog (Shh) molecule that are important for Patched (Ptc) receptor binding by targeting selected surface amino acid residues with probes of diverse sizes and shapes assessing effects these modifications on function. Eleven surround protein were chosen studies mutated to cysteine residues. These cysteines then selectively modified thiol-specific probes, proteins tested activity their ability induce differentiation C3H10T1/2 cells into osteoblasts. Based...
Hedgehog proteins modulate development and patterning of the embryonic nervous system. As expression desert hedgehog receptor, patched-1, persist in postnatal adult peripheral nerves, pathway may have a role maturation maintenance system normal disease states. We measured nerve maturing diabetic rats found that diabetes caused significant reduction mRNA. Treating with sonic hedgehog–IgG fusion protein fully restored motor- sensory-nerve conduction velocities maintained axonal caliber large...
In view of the potential importance 24,25-epoxysterols as intracellular regulators 3-hydroxy-3-methylglutaryl-CoA reductase, C-24 epimers 24,25-oxidolanosterol and 24,25-epoxycholesterol were tested for their biological activity metabolism in cell cultures. All four compounds produced repression reductase cultured mouse fibroblasts (L cells), both 24(S)- 24(R),25-epoxycholesterol exhibited high affinity binding to cytosolic oxysterol-binding protein. However, epimeric 24,25-oxidolanosterols...