A5102696053- RNA Interference and Gene Delivery
- Bone Tissue Engineering Materials
- Virus-based gene therapy research
- Protein Kinase Regulation and GTPase Signaling
- Advanced biosensing and bioanalysis techniques
- Receptor Mechanisms and Signaling
- Orthopaedic implants and arthroplasty
- Advanced Proteomics Techniques and Applications
- Graphene and Nanomaterials Applications
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Parathyroid Disorders and Treatments
- Immune cells in cancer
- CAR-T cell therapy research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Extracellular vesicles in disease
- Melanoma and MAPK Pathways
- Immunotherapy and Immune Responses
- Dental Implant Techniques and Outcomes
- biodegradable polymer synthesis and properties
- Click Chemistry and Applications
- Titanium Alloys Microstructure and Properties
- Chronic Myeloid Leukemia Treatments
National Institute of Advanced Industrial Science and Technology
2016-2024
Osaka University
2013-2024
Biomedical Research Institute
2023
Kyushu University
2010-2021
Fukuoka University
2015
National Cerebral and Cardiovascular Center
2013-2015
University of Dental Medicine
2014-2015
Japan Science and Technology Agency
2007-2013
Nagasaki University
2008
St. Marianna University School of Medicine
2008
Bisphenol A (BPA; 4,4′-isopropylidenediphenol) is a well-known endocrine disruptor. Most human exposure to BPA occurs through the consumption of BPA-contaminated foods. Cardiovascular or cardiometabolic diseases such as diabetes, obesity, hypertension, acute kidney disease, chronic and heart failure are leading causes death worldwide. Positive associations have been reported between blood urinary levels cardiovascular diseases. also induces disorders dysfunctions in tissues associated with...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDesign of Polymeric Carriers for Cancer-Specific Gene Targeting: Utilization Abnormal Protein Kinase Cα Activation in Cancer CellsJeong-Hun Kang*†‡, Daisuke Asai§, Jong-Hwan Kim∥, Takeshi Mori†⊥, Riki Toita†, Tetsuro Tomiyama†, Yoji Asami†, Jun Oishi†, Yuko T. Sato†‡, Takuro Niidome†⊥, Byungdug Jun∥, Hideki Nakashima‡§, and Yoshiki Katayama*†‡⊥View Author Information Department Applied Chemistry, Faculty Engineering, Kyushu University, 744...
Herein, we report the preparation of genetically engineered protein cages (HspG41C-SP94), taken up selectively by human hepatocellular carcinoma (HCC) cells. An cage–doxorubicin (DOX) conjugate was as cytotoxic free DOX against HCC cells but much less normal hepatocytes.
Abstract This study was aimed to investigate the osseointegration ability of poly(ether ether ketone) (PEEK) implants with modified surface roughness and/or chemistry. The roughened prepared by a sandblast method, and phosphate groups on substrates were two-step chemical reaction. in vitro osteogenic activity rat mesenchymal stem cells (MSCs) developed assessed measuring cell proliferation, alkaline phosphatase activity, osteocalcin expression, bone-like nodule formation. Surface roughening...
We described herein a human hepatocellular carcinoma (HCC) cell-targeted protein cage for which the HCC-binding peptide termed SP94 was modified at surface of naturally occurred heat shock (Hsp) cage. Six types HCC-targeted Hsp cages were chemically synthesized using two heterobifunctional linker (SM(PEG)(n)) with different lengths and peptide, contained unique Cys residue N- or C-terminus peptide. These characterized matrix-assisted laser desorption/ionization-time-of-flight mass...
Both bone-forming cells and immune have pivotal roles in bone tissue repair. In this work, we prepared a Ca-modified poly(ether ether ketone) (PEEK) to enhance the osteoconductivity but mitigate response of cells. To modify Ca ions onto chemically inert PEEK, PEEK was first coated with poly(norepinephrine) followed by soaking Ca(OH)2 aqueous solution. Modification enhanced proliferation differentiation MC3T3-E1 preosteoblast human mesenchymal stem cells, had no apparent effect on MLC-6...
Poly(ether ether ketone) (PEEK) has emerged as a candidate to replace metal implants because of its satisfactory mechanical properties, radiolucency, and lack allergy. However, PEEK lacks osseointegration ability limiting clinical applications. To overcome this problem, we prepared with micro-rough surface using the sandblast method modulate property; is simple, cost-effective, already applied implants. The roughness sandblasted was about 2.3 μm, whereas that mirror-polished 0.06 μm. Rat...
The purpose of this study was to find protein kinase C (PKC) isozyme-specific peptides. A peptide library containing 1772 sequences designed using Scansite and screened by MALDI-TOF MS activity assays for PKC isozyme-specificity. (Alphatomega; H-FKKQGSFAKKK-NH(2)) with high specificity alpha relative other isozymes identified. phosphorylated a greater extent tissue lysates from B16 melanoma, HepG2, human breast cancer, which had higher levels activated alpha, when compared normal skin,...
A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged recognize the change in net charge of peptide upon phosphorylation. Despite its simple mechanism, this is sensitive robust enough to be applied evaluation inhibitors.
Biomedical applications of poly(ether ether ketone) (PEEK) are hindered by its inherent bioinertness and lack osseointegration capability. In the present study, to enhance osteogenic activity and, hence, capability PEEK, we proposed a strategy combined phosphate calcium surface-functionalization, in which ozone-gas treatment wet chemistry were used for introduction hydroxyl groups modification and/or calcium, respectively. Surface functionalization significantly elevated surface...
Protein kinase C (PKC)α plays a key role in the differentiation, proliferation and apoptosis of cancer cells, its activity is higher cells than normal cells. In present study, we investigated existence activated PKCα plasma possibility for diagnosis. Plasma samples were prepared from xenograft mouse models mice. Phosphorylation ratios PKCα-specific peptide substrate (Alphatomega) analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry was identified western...
In this work we designed a novel nano carrier, linear polyethylenimine (LPEI)-peptide conjugate, for cancer-specific expression of transgenes. The conjugate was easily synthesized by using click chemistry scheme orthogonal to the reactive side groups peptide, which is substrate protein kinase Cα (PKCα). Polyplexes conjugates with plasmid DNA (pDNA) were intact and stably dispersed even in presence cell lysate. Despite stability, polyplexes readily dissociated upon phosphorylation grafted...
Protein nanocages are self-organized complexes of oligomers whose three-dimensional architecture can been determined in detail. These structures possess nanoscale inner cavities into which a variety molecules, including therapeutic or diagnostic agents, be encapsulated. properties yield these particles suitable for new class drug delivery carrier, as bioimaging reagent that might respond to biochemical signals many different cellular processes. We report here the design, synthesis, and...
Among five C57BL/6 mice fed a high-fat diet (HFD) for 12 weeks, one mouse showed body weight (BW) similar to normal (ND)-fed mice. We compared obesity-related parameters of three groups (ND-fed mice, HFD-fed normal-weight mouse, and overweight mice), including visceral fat weight, serum levels total cholesterol (TC), glucose, aminotransferases (AST ALT), adipocyte size, percentage crown-like structures, severity hepatic steatosis, number inflammatory foci. Compared ND-fed the exhibited...