A5070307601- Enzyme Structure and Function
- Marine Invertebrate Physiology and Ecology
- Biofuel production and bioconversion
- Marine Sponges and Natural Products
- Global trade and economics
- Antimicrobial Peptides and Activities
- Fungal and yeast genetics research
- Marine Toxins and Detection Methods
- Microbial Metabolic Engineering and Bioproduction
- Fermentation and Sensory Analysis
- Protein Hydrolysis and Bioactive Peptides
- Ion channel regulation and function
- Biochemical Acid Research Studies
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Tryptophan and brain disorders
- Pharmacological Effects of Natural Compounds
- Microbial Community Ecology and Physiology
- Collagen: Extraction and Characterization
- Helicobacter pylori-related gastroenterology studies
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Economic Zones and Regional Development
- Bacterial Genetics and Biotechnology
- Venomous Animal Envenomation and Studies
Tokyo University of Marine Science and Technology
2013-2024
Kanagawa Prefecture Education Center
2022
Japan Women's University
2021
Japan External Trade Organization
2010-2019
Osaka University
1978-2014
University of Hyogo
2013
SPring-8
2005-2013
Kyoto Prefectural University
2013
Shiseido Group (Japan)
2004-2007
Kanagawa University
2006
The present study was intended to develop a new oral mucosal dosage form with view solving the problems of administration insulin by injections. We prepared potentially suitable and tested it in beagle dogs. consists core-base, which contains cacao butter, additive, peripheral-base, mixture hydroxypropyl cellulose-H (HPC) Carbopol-934 (CP). mixing ratio HPC CP peripheral-base chosen as 1 : 2 on basis experimental results concerning stickiness, dissolution properties, viscosity fracture...
In the present study, as a continuation of previous work on new mucosal dosage form for insulin using hydroxypropyl cellulose-H (HPC) and Carbopol-934 (CP), we attempted to develop containing local anesthetic toothache, lidocaine model drug in HPC CP peripheral base. The dissolution profile was investigated with various mixing ratios freeze-dried HPC/CP (FD-HPC/CP) core base, absorption when this applied human gingiva also studied. It found that rate test delayed increase amount FD-HPC/CP...
A new type of highly viscous ointment containing Carbopol-934 (CP) for application to the oral mucosa was formulated. The release properties Brilliant Blue FCF (B.B.FCF) and absorption sodium salicylate through from were studied. In test using agar gel bed method, there no significant difference in % B.B. after 12 h ointments glycerin polyethylene glycol (PEG) as excipients. However, form maintained 24 whereas PEG liquefied. 50% time (T50) decreased with increase content, but water...
Fish skin mucus contains a variety of antimicrobial proteins and peptides that seem to play role in self defense. We previously reported an antibacterial protein the secretion rockfish, Sebastes schlegeli , which showed selective activity against Gram‐negative bacteria. This study aimed isolate structurally functionally characterize this protein. The protein, termed SSAP ( S. protein), was purified homogeneity by lectin affinity column chromatography, anion‐exchange HPLC hydroxyapatite HPLC....
The ATP hydrolysis of the V1-ATPase Thermus thermophilus have been investigated with an ATP-regenerating system at 25 °C. ratio ATPase activity to concentration ranged from 40 4000 μm; this, apparent Km 240 ± 24 μm and a Vmax 5.2 0.5 units/mg were deduced. An negative cooperativity, which is frequently observed in case F1-ATPases, was not for V1-ATPase. Interestingly, rate decayed rapidly during hydrolysis, finally stopped. Furthermore, inactivation attained by prior incubation ADP-Mg....
The major allergen (Tur c 1) in the muscle of gastropod, Turbo cornutus, was isolated by Sephacryl S-300, Mono Q HR 5/5 and TSKgel Phenyl-5PW RP column chromatography. ELISA showed Tur 1 to react strongly with sera from three individuals sensitive both mollusks crustaceans. SDS-PAGE produce a band corresponding molecular mass 35 kDa under reduced condition. Its amino acid composition characterized abundance Glx, followed Leu, Ala Lys decreasing abundance, absence Trp. In addition these...
The aim of this study was to investigate the availability an oral ointment base which contains Carbopol-934 (CP) and sticks mucosa for treatment aphtha. (CP ointment) prepared by mixing 0, 10, 20 or 30% (w/w) CP with following three kinds bases : white petrolatum (WP), hydrophilic (HP), absorptive (AO). Finally, prednisolone added each as a model drug. in vitro pharmaceutical properties ointments were evaluated penetrating, shearing stickiness drug release tests. An vivo absorption test...
Benzo[a]pyrene (BaP) is one of the polycyclic aromatic hydrocarbons, and has serious detrimental effects on human health aquatic environments. In this study, we isolated nine bacterial strains capable degrading BaP from Tokyo Bay area Tama River in Japan. The bacteria belonged to phyla Actinobacteria, Firmicutes, Proteobacteria Bacteroidetes, indicating that BaP-degrading were widely present hydrosphere. ITB11, which shared 100% 16S rRNA identity with Mesoflavibacter zeaxanthinifaciens...
A potassium channel toxin (AeK) was isolated from the sea anemone Actinia equina by gel filtration on Sephadex G‐50 and reverse‐phase HPLC TSKgel ODS‐120T. AeK α‐dendrotoxin inhibited binding of 125 I‐α‐dendrotoxin to rat synaptosomal membranes with IC 50 22 0.34 nM, respectively, indicating that is about sixty‐five times less toxic than α‐dendrotoxin. The complete amino acid sequence elucidated; it composed 36 residues including six half‐Cys residues. determined showed analogous three...
ABSTRACT The major allergen ( Cra g 1) isolated from the oyster Crassostrea gigas was digested with lysylendopeptidase. Twenty‐one peptide fragments were digest by reverse‐phase HPLC and some of them sequenced. 1 identified as tropomyosin. In competitive ELISA inhibition analyses lysylendopeptidase fragments, a (K21) showed maximum binding IgE antibodies in sensitive human serum to 1. On further tryptic hydrolysis K21, two peptides exhibited high inhibitory ability equivalent K21. These...
Journal Article Purification and properties of a histidine decarboxylase from Tetragenococcus muriaticus, halophilic lactic acid bacterium Get access Y. Konagaya, Konagaya Department Food Science Technology, Tokyo University Fisheries, Minato‐ku, Tokyo, Japan Search for other works by this author on: Oxford Academic Google Scholar B. Kimura, Kimura M. Ishida, Ishida T. Fujii Applied Microbiology, Volume 92, Issue 6, 1 June 2002, Pages 1136–1142,...
ABSTRACT In this study, we demonstrate that Porphyromonas gingivalis fimbriae use molecules of β 2 integrin (CD11/CD18) on mouse peritoneal macrophages as cellular receptors and also show the chain (CD18) may play a functional role in signalling for fimbria-induced expression interleukin-1β (IL-1β) tumor necrosis factor alpha (TNF-α) genes cells. Using binding assay with 125 I-labeled fimbriae, observed fimbrial to was inhibited by treatment CD11a, CD11b, CD11c, or CD18 antibody but not CD29...