A5026355017- Aquaculture disease management and microbiota
- Antimicrobial Peptides and Activities
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- Physiological and biochemical adaptations
- Environmental Toxicology and Ecotoxicology
- Bone Metabolism and Diseases
- Protein Hydrolysis and Bioactive Peptides
- Meat and Animal Product Quality
- Toxic Organic Pollutants Impact
- Neuroendocrine regulation and behavior
- Fluoride Effects and Removal
- Bone health and osteoporosis research
- Cephalopods and Marine Biology
- Bone and Dental Protein Studies
- Birth, Development, and Health
- Bone health and treatments
- Marine Biology and Environmental Chemistry
- Silicon and Solar Cell Technologies
- Zebrafish Biomedical Research Applications
- Echinoderm biology and ecology
- Regulation of Appetite and Obesity
- Neuropeptides and Animal Physiology
- Molecular Biology Techniques and Applications
- Galectins and Cancer Biology
Kanazawa University
2016-2023
Nihon University
2012-2020
Nord University
2013-2017
Kyushu University
2017
Osaka University
2014
Tokyo University of Marine Science and Technology
2006-2013
Tokyo Ohka Kogyo (Japan)
1991
A novel defensin antimicrobial peptide gene was identified in Atlantic cod, Gadus morhua. This three exon/two intron codes for a precursor consisting of two domains: signal 26 amino acids and mature 40 residues. The cod has six conserved cysteine residues that form 1-5, 2-4 3-6 disulphide bridges. pattern is typical beta-defensins this therefore named beta-defensin (defb). tertiary structure Defb exhibits an α/β fold with one α helix β1β2β3 sheets. RT-PCR analysis indicated defb transcripts...
The efficacy of a microbial feed additive (Bactocell(®)) in countering intestinal inflammation Atlantic salmon was examined this study. Fish were fed either the additive-coated (probiotic) or without it (control). After an initial 3-week feeding, inflammatory condition induced by anally intubating all fish with oxazolone. offered feeds for 3 more weeks. Distal intestine from groups obtained at 4 h, 24 and weeks, after oxazolone treatment. Inflammatory responses prominent both documented...
Abstract The threat of predation is a driving force in the evolution animals. We have previously reported that Xenopus laevis enhanced their tail muscles and increased swimming speeds presence Japanese larval salamander predators. Herein, we investigated induced gene expression changes brains tadpoles under using 3′-tag digital profiling. found many muscle genes were expressed after 24 h exposure to predation. Ingenuity pathway analysis further showed threat, various signal transduction...
Fish skin mucus contains a variety of antimicrobial proteins and peptides that seem to play role in self defense. We previously reported an antibacterial protein the secretion rockfish, Sebastes schlegeli , which showed selective activity against Gram‐negative bacteria. This study aimed isolate structurally functionally characterize this protein. The protein, termed SSAP ( S. protein), was purified homogeneity by lectin affinity column chromatography, anion‐exchange HPLC hydroxyapatite HPLC....
Alkaline treatment has been extensively implemented in the extraction process of hydroxyapatite (HAp) from various kinds bio-materials, such as animal bone and scales. The main purpose is to remove proteinaceous substances raw materials. influence alkaline that could alter not only organic contents but also chemical composition—specifically Ca/P mole ratios bio-calcium, HAp, biphasic apatite powders derived salmon bone, a by-product industry—was investigated. Both HAp were obtained...
To study the toxicity of 3-hydroxybenzo[c]phenanthrene (3-OHBcP), a metabolite benzo[c]phenanthrene (BcP), first we compared it with its parent compound, BcP, using an in ovo-nanoinjection method Japanese medaka. Second, examined influence 3-OHBcP on bone metabolism goldfish. Third, detailed mechanism was investigated zebrafish and The LC50s BcP medaka were 5.7 nM 0.003 nM, respectively, indicating that more than 1900 times as toxic compound. In addition, nanoinjected (0.001 nM) induced...
Summary Growth hormone (GH) transgenic Amago (Oncorhynchus masou ishikawae), containing the sockeye GH1 gene fused with metallothionein-B promoter from same species, were generated and physiological condition through lipid metabolism compared among homozygous (Tg/Tg) heterozygous GH (Tg/+) wild type control (+/+). Previously, we have reported that adipose tissue was generally smaller in fish to control, Δ-6 fatty acyl desaturase down-regulated Tg/+ fish. However, acid (FA) compositions not...
Biocalcium powders, Bio-cal-H, and Bio-cal-A, obtained from non-alkaline treated alkaline salmon frame were characterized. Glycine was the dominant amino acid (216.45–321.80 mg/g), followed by glutamic (102.52–110.38 proline (72.96–96.10 hydroxyproline (72.98–83.75 mg/g) in both biocalcium powders. Bio-cal-H possessed a high abundance of volatile compounds such as 2-propanone, acetonitrile, 2-butanone than Bio-cal-A. Bio-cal-A had higher solubility vitro simulated gastrointestinal tract...
It has been reported that spinal deformity was induced in developing fish by the addition of polycyclic aromatic hydrocarbons (PAHs). To examine mechanism disruption bone metabolism, effect benz[a]anthracene (BaA), a kind PAH, on plasma calcium, inorganic phosphorus, osteoblasts, and osteoclasts investigated this study. We also measured several components to analyze toxicity BaA other metabolisms. (1 or 10 ng/g body weight) intraperitoneally injected (four times) into nibbler during...
The mucus of fish skin plays a vital role in innate immune defense. Some proteins have the potential to incapacitate pathogens and/or inhibit their passage through skin. In this study aim was isolate and characterize galectin(s), β-galactosides binding proteins, present mucus. A novel short form galectin-3 isolated from Atlantic salmon by α-lactose agarose based affinity chromatography followed Sephadex G-15 gel filtration. Mass spectrometric analysis showed that protein C-terminal half...
Oxygen deprivation induces multiple changes at the cellular and organismal levels, its re-supply also brings another special physiological status. We have investigated effects of hypoxia/re-oxygenation on embryonic growth using zebrafish model: hypoxia slows growth, but re-oxygenation spurt or catch-up . The mitogen-activated kinase (MAPK)-pathway downstream insulin-like factor (IGF/Igf) has been revealed to positively regulate re-oxygenation-induced role reactive oxygen species generated by...
Abstract This study is the first to demonstrate that deep ocean water (DOW) has physiological significant effects on squid. After 36 h of rearing squids, those reared with DOW had significantly higher total and free cholesterol levels lower alanine transaminase activity in hemolymph as compared surface sea (SSW). SSW also resulted 6.95% weight loss, while caused only 2.5% which might be due liver metabolism suppression. Furthermore, both monovalent (sodium, chloride, potassium ions) divalent...
Summary The rapid induction of a defensive morphology by prey species in face predation risk is an intriguing ecological context; however, the physiological mechanisms that underlie this phenotypic plasticity remain uncertain. Here we investigated changes shown Rana pirica tadpoles response to threat larvae salamander Hynobius retardatus. One such bulgy morph phenotype, relatively swelling size begins within 4 days and reaches maximum at 8 10 days. We found although total volume bodily fluid...