A5002540740- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Acute Lymphoblastic Leukemia research
- Monoclonal and Polyclonal Antibodies Research
- Estrogen and related hormone effects
- Protein Kinase Regulation and GTPase Signaling
- Chronic Myeloid Leukemia Treatments
- Chemical Synthesis and Analysis
- Receptor Mechanisms and Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- HIV Research and Treatment
- Acute Myeloid Leukemia Research
- Connective tissue disorders research
- Effects and risks of endocrine disrupting chemicals
- Melanoma and MAPK Pathways
- Digital Accessibility for Disabilities
- Advanced Proteomics Techniques and Applications
- Click Chemistry and Applications
- Mobile Health and mHealth Applications
- Technology Use by Older Adults
- Circadian rhythm and melatonin
- Cell death mechanisms and regulation
- Cancer Research and Treatments
- Usability and User Interface Design
Showa Pharmaceutical University
2021-2024
Meiji University
2023-2024
Hamamatsu University School of Medicine
2020-2023
Kyushu University
1999-2021
St. Marianna University School of Medicine
2012-2021
Japanese Red Cross Society Kyoto Daini Hospital
2016-2018
Kawasaki Hospital
2018
Fukuyama City Hospital
2014-2015
Kyoto Prefectural University of Medicine
2011-2015
North Medical Center
2015
Bisphenol A (BPA; 4,4′-isopropylidenediphenol) is a well-known endocrine disruptor. Most human exposure to BPA occurs through the consumption of BPA-contaminated foods. Cardiovascular or cardiometabolic diseases such as diabetes, obesity, hypertension, acute kidney disease, chronic and heart failure are leading causes death worldwide. Positive associations have been reported between blood urinary levels cardiovascular diseases. also induces disorders dysfunctions in tissues associated with...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDesign of Polymeric Carriers for Cancer-Specific Gene Targeting: Utilization Abnormal Protein Kinase Cα Activation in Cancer CellsJeong-Hun Kang*†‡, Daisuke Asai§, Jong-Hwan Kim∥, Takeshi Mori†⊥, Riki Toita†, Tetsuro Tomiyama†, Yoji Asami†, Jun Oishi†, Yuko T. Sato†‡, Takuro Niidome†⊥, Byungdug Jun∥, Hideki Nakashima‡§, and Yoshiki Katayama*†‡⊥View Author Information Department Applied Chemistry, Faculty Engineering, Kyushu University, 744...
Abstract Genetic alterations of Ikaros family zinc finger protein 1 ( IKZF ), point mutations in Janus kinase 2 JAK and overexpression cytokine receptor‐like factor CRLF ) were recently reported to be associated with poor outcomes pediatric B‐cell precursor BCP )‐ ALL . Herein, we conducted genetic analyses deletion, mutation exon 16, 17, 21, expression, the presence P2 RY 8‐ fusion F232C 202 ‐ patients newly diagnosed registered Japan Childhood Leukemia Study 02 protocol find out if these...
Octyl‐ and nonylphenols in the environment have been proposed to function as estrogens. To gain insight into their structural essentials binding estrogen receptor, a series of phenols with saturated alkyl groups at para position, HO‐C 6 H 4 ‐C n 2n+1 ( = 0–12), were examined for ability displace [ 3 H]17β‐estradiol recombinant human which was expressed Sf9 cells using vaculovirus expression system. All tested ‐alkylphenols found bind fully receptors dose‐dependent manner. The interaction...
Brachytherapy is a common clinical technique involving implantation of sealed radioactive "seeds" within tumor to selectively irradiate the mass while minimizing systemic toxicity. To mitigate disadvantages associated with complex surgical and subsequent device removal procedures, we have developed an alternative approach using genetically encoded peptide polymer solution composed thermally responsive elastin-like polypeptide (ELP) radiolabeled (131)I that self-assembles into radionuclide...
The rheological properties of cysteine-containing elastin-like polypeptide (Cys-ELP) solutions and Cys-ELP hydrogels are reported. exhibit a surprisingly high apparent viscosity at low shear rate. is attributed to the formation an interfacial cross-linked "skin" sample surface, rather than bulk solution. At higher rate, film breaks, its influence on solution can be ignored. formed by mixing hydrogen peroxide (H2O2). fixed concentration Cys-ELP, gelation time tuned H2O2. have typical...
The purpose of this study was to find protein kinase C (PKC) isozyme-specific peptides. A peptide library containing 1772 sequences designed using Scansite and screened by MALDI-TOF MS activity assays for PKC isozyme-specificity. (Alphatomega; H-FKKQGSFAKKK-NH(2)) with high specificity alpha relative other isozymes identified. phosphorylated a greater extent tissue lysates from B16 melanoma, HepG2, human breast cancer, which had higher levels activated alpha, when compared normal skin,...
Protein kinase C (PKC)α plays a key role in the differentiation, proliferation and apoptosis of cancer cells, its activity is higher cells than normal cells. In present study, we investigated existence activated PKCα plasma possibility for diagnosis. Plasma samples were prepared from xenograft mouse models mice. Phosphorylation ratios PKCα-specific peptide substrate (Alphatomega) analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry was identified western...
For an overall characterization of pediatric B-cell precursor acute lymphoblastic leukemia (BCPALL) with CRLF2 overexpression (OE), we conducted genetic analysis in 167 BCPALL patients. OE was detected 30 (18%) patients, the P2RY8-CRLF2 fusion identified only 3 (1.8%) all which demonstrated OE. Moreover, gain 18 (11%) Messenger RNA sequencing revealed a novel transcript, CSF2RA-CRLF2, case OE, suggesting that this is associated In survival analysis, no significant differences 5-year...
Abstract This study reviewed the clinical characteristics of 112 pediatric B‐cell precursor acute lymphoblastic leukemia ( BCP ‐ ALL ) patients with TCF 3‐ PBX 1 fusion treated according to Japan Association Childhood Leukemia Study JACLS 02 protocol n = 82) and Children's Cancer Group CCLSG 2004 30). The 3‐year event‐free survival EFS overall OS rates were 85.4 ± 3.9% 89.0 3.5% in cohort, 5‐year 82.8 7.0% 86.3 6.4% respectively, which are comparable those reported western countries....
Activating tyrosine kinase mutations or cytokine receptor signalling alterations have attracted attention as therapeutic targets for high-risk paediatric acute lymphoblastic leukaemia (ALL). We identified two novel fusions, OFD1-JAK2 and NCOR1-LYN, in ALL patients with IKZF1 deletion, by mRNA sequencing. The patient CSF2RA-CRLF2 also harboured IGH-EPOR. All these had features, such high initial white blood cell counts poor response to prednisolone. functional analysis of fusions is on-going...
Sediment transports due to tsunamis have caused serious damages. On the other hand, they can be used analyze historical tsunamis. A model evaluate erosion, deposition and sand movement of is proposed in this study. hydraulic experiment was carried out develop it applied 1960 Chilean Tsunami Kesennuma Bay Japan.
P-glycoprotein (Pgp) is a 170-kDa transmembrane protein that mediates the efflux of anticancer drugs from cells. Pgp overexpression has distinct role in cells exhibiting multidrug resistance (MDR). We examined reversal drug human MDR breast cancer by inhibition kinase Cα (PKCα) activity, which associated with Pgp-mediated drugs. PKCα activity was confirmed measurement phosphorylation levels PKCα-specific peptide substrate (FKKQGSFAKKK-NH2), showing relatively higher basal drug-resistant...
Abstract Background Control of inflammation is essential for the clinical management many common human diseases. However, there are few generally applicable strategies to convert an abnormal intracellular signal into a gene expression that leads normalization environment. Recently, we proposed novel strategy termed D‐RECS (i.e. drug or delivery system responding cellular signals) transgene expression. In present study, applied this concept inflammatory cells using Iκ‐B kinase as molecule...
Peptide substrates sensitive for a certain protein kinase could be important new-drug development and to understand the mechanism of diseases. Rho-associated (Rho-kinase/ROCK) is serine/threonine kinase, plays an part in cardiovascular disease, migration invasion tumor cells, neurological disorders. The purpose this study was find with high affinity sensitivity ROCK2. We synthesized 136 peptide from ROCK2 different lengths charged peptides. Incorporation 32P [counts per minute (CPM)] each...
We propose a medication reminder system which supports users' compliance. Poor compliance of causes significant problems including worsening disease and increase healthcare costs. One common reason for poor is patients' forgetfulness. identified four types failures caused by forgetfulness requires the to consider various behaviors other than consumption medication, while existing systems only medication. The architecture designed work with sensors actuators, enable vast variety behaviors....
G protein‐coupled receptor kinases (GRKs) control the signaling and activation of receptors through phosphorylation. In this study, consensus substrate motifs for GRK2 were identified from sequences protein substrates, 17 candidate peptides synthesized to identify peptide substrates with high affinity GRK2. appears require an acidic amino acid at −2, −3, or −4 positions its phosphorylation site as (D/E)X 1–3 ( S / T ), )(D/E), 0–2 (D/E)( ). Among examined, a 13‐amino‐acid fragment β‐tubulin...