A5035340824- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- Childhood Cancer Survivors' Quality of Life
- Hematopoietic Stem Cell Transplantation
- Chronic Myeloid Leukemia Treatments
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Circadian rhythm and melatonin
- Pancreatic and Hepatic Oncology Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Neuroscience and Neuropharmacology Research
- Adolescent and Pediatric Healthcare
- Enzyme function and inhibition
- Neuroblastoma Research and Treatments
- Glycosylation and Glycoproteins Research
- Lung Cancer Research Studies
- Cholinesterase and Neurodegenerative Diseases
- Pancreatic function and diabetes
- Retinoids in leukemia and cellular processes
- Cancer, Hypoxia, and Metabolism
- Polyamine Metabolism and Applications
- Cancer-related gene regulation
- Immunodeficiency and Autoimmune Disorders
National Center For Child Health and Development
2018-2025
Mie University
2015-2024
National Institute for Japanese Language and Linguistics
2023-2024
Children's Cancer Center
2022-2024
Mie University Hospital
2015-2017
Japan Pediatric Society
2016
National Institute of Advanced Industrial Science and Technology
2015
Nikki-Universal (Japan)
2012
Japan Medical Association
2011
Medical University of South Carolina
2001-2004
To elucidate the molecular basis of human N-acetylation polymorphism, cDNA clones encoding liver N-acetyltransferases (EC 2.3.1.5) were isolated from lambda gt10 libraries using 32P-labeled rabbit N-acetyltransferase recently cloned in this laboratory. Three types cDNAs (D-14, O-7, and D-24) their nucleotide sequences determined, which amino acid deduced. All coded for 290 acids. Between D-14 O-7 cDNAs, there was only a single-base substitution coding region, replaced glutamic glycine cDNA....
Southern blot analysis was performed with genomic DNAs from 86 human subjects using the 32P-labeled cDNA for polymorphic arylamine N-acetyltransferase (EC 2.3.1.5) in liver recently cloned our laboratory. Three types of gene were identified. Gene 1 contains a 5.5-kilobase (kb) KpnI fragment BamHI site; 2 5.5-kb without and 3 5.0-kb site. The combination these three genes generated five genotypes. Acetylator phenotypes determined 29 healthy volunteers by isoniazid loading tests, they...
Phosphatidic acid added to the medium markedly elevated intracellular cyclic GMP content in cultured neuroblastoma N1E 115 cells.There was a significant elevation of with 1 pg/ml and maximum (70-fold) 100 phosphatidic acid.Other natural phospholipids did not increase, or increased only slightly, cells.The by absolutely dependent on extracellular calcium.Phosphatidic stimulated influx calcium into cells 2-to 6-fold.The pattern induced comparable that elevation.The stimulation also observed...
Guanylatecyclase in neuroblastoma N1E 115 cells was readily solubilized upon homogenization of the with hypotonic buffer.When supernatant passed through cation exchangers such as a Chelex 100 Na+ column, guanylate cyclase activity effluent fraction decreased to 4-6% original supernatant.The addition acid extract or rat tissues restored activity, indicating that contained an acid-soluble endogenous activator for which adsorbed on exchangers.The purified from brain and identified L-arginine by...
Seven types of mRNA for choline acetyltransferase that differ in the 5'-noncoding region were identified mouse spinal cord by cDNA cloning and polymerase chain reaction. Among these transcripts, M-type corresponding to previously cloned was most abundant mouse. A genomic DNA clone containing 5'-region isolated sequenced. Comparison sequences between cDNAs revealed different species transcribed from promoter regions produced differential splicing. Two murine cholinergic cell lines, NS20Y...
Serotonin:acetyl coenzyme A N-acetyltransferase (EC 2.3.1.5) Activity in pineal gland was assayed rats which were born and raised under diurnal lighting conditions, continuous darkness, or constant light. N-Acetyltransferase activity the pups showed a rhythmic pattern, with high enzyme during dark period. The darkness also phase of delayed by 8 hr 7-week-old pups; began evening 12-day-old regularly 1 every week. illumination pattern; 3 When mother coupled maintained pregnancy after born,...
Recent studies revealed that a substantial proportion of patients with high-risk B-cell precursor acute lymphoblastic leukemia (BCP-ALL) harbor fusions involving tyrosine kinase and cytokine receptors, such as ABL1, PDGFRB, JAK2 CRLF2, which are targeted by inhibitors (TKIs). In the present study, transcriptome analysis or multiplex reverse transcriptase-PCR 373 BCP-ALL without recurrent genetic abnormalities identified 29 fusions. Clinically, male predominance (male/female: 22/7), older age...
Fifty human livers obtained at autopsy were analyzed for N-acetyltransferase and classified into six genotypes. Determination of activity proteins from supernatants liver homogenates indicate that genotype I corresponds to rapid acetylator, genotypes II III intermediate IV, V, VI slow acetylator phenotypes. Northern blot analysis shows levels mRNA in the do not markedly differ among Three alleles gene cloned sequenced. is coded two exons. Comparison 2 3, which correspond low activity, with...
Abstract cDNAs containing the entire coding regions of alpha and beta subunits calmodulin-dependent protein kinase II (CaM II) were isolated from a rat cerebrum cDNA library, ligated into an expression vector under control SV40 early promoter introduced Chinese hamster ovary (CHO) cells. To investigate role their functional domains in CaM activity, properties kinases expressed transfected cells studied. activity was detected when CHO simultaneously. RNA transfer blot immunoblot analyses...
Abstract Genetic alterations of Ikaros family zinc finger protein 1 ( IKZF ), point mutations in Janus kinase 2 JAK and overexpression cytokine receptor‐like factor CRLF ) were recently reported to be associated with poor outcomes pediatric B‐cell precursor BCP )‐ ALL . Herein, we conducted genetic analyses deletion, mutation exon 16, 17, 21, expression, the presence P2 RY 8‐ fusion F232C 202 ‐ patients newly diagnosed registered Japan Childhood Leukemia Study 02 protocol find out if these...
Abstract Recent genetic studies using high-throughput sequencing have disclosed alterations in B-cell precursor acute lymphoblastic leukemia (B-ALL). However, their effects on clinical outcomes not been fully investigated. To address this, we comprehensively examined and prognostic impact a large series of pediatric B-ALL cases. We performed targeted capture total 1003 patients with from 2 Japanese cohorts. Transcriptome (n = 116) and/or array-based gene expression analysis 120) were also...
KMT2A-rearranged infant acute lymphoblastic leukemia (ALL) represents the most refractory type of childhood leukemia. To uncover molecular heterogeneity this disease, we perform RNA sequencing, methylation array analysis, whole exome and targeted deep sequencing on 84 infants with Our multi-omics clustering followed by single-sample single-cell inference hematopoietic differentiation establishes five robust integrative clusters (ICs) different master transcription factors, fusion partners...
Mixed lineage leukemia 1-rearranged (MLL1-r) acute patients respond poorly to currently available treatments and there is a need develop more effective therapies directly disrupting the Menin‒MLL1 complex. Small-molecule-mediated inhibition of protein‒protein interaction between Menin MLL1 fusion proteins potential therapeutic strategy for with MLL1-r or mutated-nucleophosmin 1 (NPM1c) leukemia. In this study, we preclinically evaluated new compound DS-1594a its salts.We preclinical efficacy...
Abstract Acute lymphoblastic leukemia expressing the gamma delta T-cell receptor (γδ T-ALL) is a poorly understood disease. We studied 200 children with γδ T-ALL from 13 clinical study groups to understand and genetic features of this found age drivers were significantly associated outcome. diagnosed in under 3 years was extremely high-risk enriched for alterations that result both LMO2 activation STAG2 inactivation. Mechanistically, using patient samples isogenic cell lines, we show...
Background Acute lymphoblastic leukemia (ALL)-based therapeutic regimens have markedly improved the survival of children with ALL and lymphoma (LBL). However, survivors are at risk secondary neoplasms. Few studies on such neoplasms been conducted outside Europe United States. The aim this study was to evaluate incidence of, factors for, outcomes in long-term LBL a tertiary pediatric oncology center Mie prefecture, Japan. Procedure We retrospectively reviewed 188 patients who were treated an...
When the crude mitochondrial fraction of rat brain was homogenized with distilled water and centrifuged, most guanylate cyclase activity detected in soluble fraction. The total recovered 5- to 8-fold higher than that greater recovery found be due a release an endogenous activating factor for cyclase. partially purified by acid extraction followed gel filtration ion exchange resin columns. dialyzable small molecule. molecular weight estimated between 300 600 Sephadex G-15 column Diaflo...