A5038860978- Heme Oxygenase-1 and Carbon Monoxide
- Hemoglobin structure and function
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Neonatal Health and Biochemistry
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Enzyme Production and Characterization
- Eicosanoids and Hypertension Pharmacology
- Neuropeptides and Animal Physiology
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Alcohol Consumption and Health Effects
- Porphyrin Metabolism and Disorders
- Receptor Mechanisms and Signaling
- Studies on Chitinases and Chitosanases
- Photosynthetic Processes and Mechanisms
- Enzyme Catalysis and Immobilization
- Aldose Reductase and Taurine
- Chemical Synthesis and Reactions
- Matrix Theory and Algorithms
- Peptidase Inhibition and Analysis
- Metal-Catalyzed Oxygenation Mechanisms
- Synthesis and Characterization of Heterocyclic Compounds
- Biofuel production and bioconversion
GlyTech
2024-2025
Tohoku University
2014-2023
Toho University
2002-2023
Kurume University
2005-2021
Japan Tobacco (Japan)
1992-2021
Keio University
2021
Sendai University
2021
Graduate School USA
2021
GlyTech (United States)
2019
Tokai University
2012-2018
The luminescent jellyfish Aequorea contains a photoprotein, aequorin, which emits light by an intramolecular reaction in the presence of trace amount Ca2+. A cDNA library was constructed and clones carrying for Ca2+-dependent photoprotein were isolated method colony hybridization using synthetic oligonucleotide probes. primary structure protein deduced from nucleotide sequence showed that is composed 189 amino acid residues has three E-F hand structures are characteristic Ca2+-binding sites....
Various beta-glycosyl azides have been synthesized directly in water by the reaction of unprotected sugars and sodium azide mediated 2-chloro-1,3-dimethylimidazolinium chloride (DMC).
Sugar oxazoline derivatives were directly synthesized from the corresponding N-acetyl-2-amino sugars in aqueous media by using a chloroformamidinium-type dehydrating reagent. The present method could successfully be applied to chito-oligosaccharides, saccharides with acid functions, and complex-type oligosaccharide derived glycopeptide.
Heme oxygenase catalyzes the oxidative cleavage of protoheme to biliverdin, first step heme metabolism utilizing O 2 and NADPH. We determined crystal structures rat oxygenase‐1 (HO‐1)–heme selenomethionyl HO‐1–heme complexes. is sandwiched between two helices with δ‐ meso edge being exposed surface. Gly143N forms a hydrogen bond distal ligand heme, OH − . The distance shorter than that in human complex. This difference may be related pH‐dependent change heme. Flexibility helix control...
The oxygenated form of the hememheme oxygenase complex was obtained by passing carbon monoxide reduced heme.heme through a column Sephadex G-25.The absorption spectrum closely resembled oxyhemoglobin and showed maxima at 412, 540, 575 nm.The heme bound to quite stable neutral pH in air but readily auto-oxidizable 6.0.There no indication degradation during auto-oxidation heme, when incubated with NADPH-cytochrome c reductase system, quantitatively converted biliverdin, indicating that...
The magnitude of the slow phase reaction cytochrome oxidase with cyanide has been correlated size epr signal at g' = 12.This was not found in submitochondrial particles, and significant 12 only observed late
Significance Heme oxygenase (HO) is a key enzyme for heme degradation that deeply involved in iron homeostasis, defensive reaction against oxidative stress, and signal transduction mediated by carbon monoxide. To complete single HO reaction, seven electrons supplied from NADPH-cytochrome P450 reductase (CPR) are required. Based on crystallography, X-ray scattering, NMR analyses of CPR, it has been proposed CPR dynamic equilibrium the “closed-open transition.” The crystal structure transient...
Human MCs are primary effectors implicated in immune surveillance and defense by secreting histamine various inflammatory mediators, a mechanism termed as degranulation. can be activated two pathways: IgE-dependent classical pathway the IgE-independent that utilizes cationic molecules including substance P (SP) pituitary adenylate cyclase-activating polypeptides, which host peptides collectively known basic secretagogues. Our pharmacological study investigated whether or not MC activation is...
The peptidylglycine alpha-amidating enzyme catalyzes a reaction that transforms carboxyl-terminal glycine-extended precursor into alpha-amidated peptide. We purified an from equine serum by simplified steps including substrate affinity chromatography. With the enzyme, we detected intermediate of high performance liquid chromatography analysis. production required copper, oxygen, and ascorbate increased linearly with incubation time. structure was determined to be hydroxyl derivative at...
The discovery of new chlorophyllous pigments would provide greater understanding the mechanisms and evolution photosynthesis. Bacteriochlorophyll f has never been observed in nature, although this name was proposed ~40 years ago based on structurally related compounds. We constructed a bacteriochlorophyll f–accumulating mutant green sulfur bacterium Chlorobaculum limnaeum, which originally produced e, by knocking out bchU gene encoding C-20 methyltransferase natural transformation. This...
Abstract A facile and practical method for synthesis of sugar oxazolines (=dihydrooxazoles) from the corresponding N ‐acetyl‐2‐amino sugars has been developed by using 2‐chloro‐1,3‐dimethyl‐1 H ‐benzimidazol‐3‐ium chloride (CDMBI) as a dehydrative condensing agent. The intramolecular reaction between 2‐acetamido group anomeric OH unprotected took place smoothly in 2 O, leading to formation 1,2‐oxazoline (=4,5‐dihydrooxazole) moiety good yield. Since proceeds O without any protecting groups,...
A novel series of RORγ inhibitors was identified starting with the HTS hit 1. After SAR investigation based on a prospective consideration two drug-likeness metrics, ligand efficiency (LE) and fraction sp(3) carbon atoms (Fsp(3)), significant improvement metabolic stability as well reduction CYP inhibition observed, which finally led to discovery selective orally efficacious inhibitor 3z.