A5071035893- Plant Reproductive Biology
- Plant Physiology and Cultivation Studies
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant Pathogens and Fungal Diseases
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Horticultural and Viticultural Research
- Plant Genetic and Mutation Studies
- Mitochondrial Function and Pathology
- Fungal Plant Pathogen Control
- Plant Virus Research Studies
- Neuropeptides and Animal Physiology
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Neurogenesis and neuroplasticity mechanisms
- Oral microbiology and periodontitis research
- Genetic Neurodegenerative Diseases
- Virus-based gene therapy research
- Nerve injury and regeneration
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Enzyme Production and Characterization
- Nutrition, Genetics, and Disease
Institute of Agrobiological Sciences
2008-2023
National Agriculture and Food Research Organization
2001-2019
Biotherapy Institute of Japan
2018-2019
Hokkaido University
2015
Genetique Reproduction and Developpement
2012
Institute of Fruit Tree and Tea Science
2006-2011
Apple (Israel)
2007
University of Pittsburgh
2005-2006
Université Clermont Auvergne
2006
Centre National de la Recherche Scientifique
2006
Abstract To enhance our understanding of GA metabolism in rice (Oryza sativa), we intensively screened and identified 29 candidate genes encoding the following metabolic enzymes using all available DNA databases: ent-copalyl diphosphate synthase (CPS), ent-kaurene (KS), oxidase (KO), ent-kaurenoic acid (KAO), 20-oxidase (GA20ox), 3-oxidase (GA3ox), 2-oxidase (GA2ox). In contrast to Arabidopsis genome, multiple CPS-like, KS-like, KO-like were most which are contiguously arranged. We also 18...
Because retrotransposons are the major component of plant genomes, analysis target site selection is important for understanding structure and evolution genomes. Here, we examined specificity rice retrotransposon Tos17, which can be activated by tissue culture. We have produced 47,196 Tos17-induced insertion mutants rice. This mutant population carries ∼500,000 insertions. analyzed >42,000 flanking sequences newly transposed Tos17 copies from 4316 lines. More than 20,000 unique loci were...
To study the role of suprachiasmatic nuclei (SCN) in generating circadian rhythms female rats, lesions were placed SCN or medial preoptic (PO) region. Serial blood sampling at 4-hour intervals 3 and 13 weeks after surgery indicated that complete destruction abolished rhythmic fluctuations plasma corticosterone levels individual rats. Partial produced less interference, while PO spared without effect. Similar effects noted on daily changes body temperature 10 surgery; however, some rats...
Abstract Plasticity of root growth in response to environmental cues and stresses is a fundamental characteristic land plants. However, the molecular basis underlying regulation under stressful conditions poorly understood. Here, we report that rice nuclear factor, RICE SALT SENSITIVE3 (RSS3), regulates cell elongation during adaptation salinity. Loss function RSS3 only moderately inhibits normal conditions, but it provokes spontaneous swelling, accompanied by severe inhibition, saline...
Plant growth and development are sustained by continuous cell division in the meristems, which is perturbed various environmental stresses. For maintenance of meristematic functions, it essential that be coordinated with differentiation. However, unknown how proliferative activities meristems coordination between differentiation maintained under stressful conditions. Here we show a rice protein, RSS1, whose stability controlled cycle phases, contributes to vigour cells viability salinity...
Abstract Rad51 is a homolog of the bacterial RecA recombinase, and key factor in homologous recombination eukaryotes. paralogs have been identified from yeast to vertebrates. are thought play an important role assembly or stabilization that promotes pairing strand exchange reactions. We previously characterized two RAD51 paralogous genes Arabidopsis (Arabidopsis thaliana) named AtRAD51C AtXRCC3, which homologs human RAD51C XRCC3, respectively, described interaction their products two-hybrid...
Abstract Although Valsa canker caused by ceratosperma (Tode ex Fr.) Maire is one of the most destructive diseases in apple ( Malus × domestica Borkh.) especially eastern Asia, information available to help with breeding against apples limited. In this work, 53 accessions diverse species and their interspecific hybrids were tested for resistance V. , using an excised shoot assay. Dormant shoots succulent growing from each accession inoculated a virulent isolate AVC‐12 length necrosis was...
Understanding the flowering process in apple (Malus × domestica Borkh.) is essential for developing methods to shorten breeding period and regulate fruit yield. It known that FLOWERING LOCUS T (FT) acts as a transmissible floral inducer Arabidopsis network system. To clarify molecular of two FT orthologues, MdFT1 MdFT2, we performed yeast two-hybrid screen identify proteins interact with MdFT1. We identified several transcription factors, including members TCP (TEOSINTE BRANCHED1, CYCLOIDEA...
Summary Homologous recombination (HR) is an essential process in maintaining genome integrity and variability. In eukaryotes, the Rad52 epistasis group proteins are involved meiotic and/or HR repair. One member of this group, Rad54, belongs to SWI2/SNF2 family DNA‐stimulated ATPases. Recent studies indicate that Rad54 has important functions HR, both as a chromatin remodelling factor mediator Rad51 nucleoprotein filament. Despite importance no study from plants yet been performed. Here, we...
Two monoclonal antibodies directed to the blood group A determinant have been established. One hybridoma antibody (AH16), obtained on immunization with human gastric cancer cell MKN45, was carried by both type 1 and 2 chains. The other (AH21) prepared a purified 1-chain glycolipid, followed selection of positive reaction 1, but negative 2-chain glycolipid. Thus, specifically reacts chain, not chain; an chain differs significantly from that in its conformational structure. AH16 agglutinates...
During meiosis homologous chromosomes pair, recombine, and synapse, thus ensuring accurate chromosome segregation the halving of ploidy necessary for gametogenesis. The processes permitting a to pair only with its homologue are not fully understood, but successful pairing is tightly linked recombination. In Arabidopsis thaliana, meiotic prophase rad51, xrcc3, rad51C mutants appears normal up zygotene/pachytene stage, after which genome fragments, leading sterility. To better understand...
Oryzacystatin (OC) is the first-described cystatin originating from rice seed; it consists of two molecular species, OC-I and OC-II, which have antiviral action against poliovirus in vitro (H. Kondo, S. Ijiri, K. Abe, H. Maeda, Arai, FEBS Lett. 299:48-50, 1992). In experiments reported here, we investigated effects OC-II on replication herpes simplex virus type 1 (HSV-1) vivo. HSV-1 was inoculated onto monolayers monkey kidney epithelial cells (CV-1 cells) at a multiplicity infection 0.1 PFU...
Rh-associated glycoprotein (RhAG) is closely associated with the Rh proteins in red cell membrane. Two high frequency antigens (Duclos and DSLK) one low antigen (Ol(a)) have serological characteristics suggestive of expression on RhAG.RHAG was sequenced from DNA Duclos-negative, DSLK-negative, two Ol(a+) individuals. Recombinant protein expressed HEK 293 cells. Protein models RhAG subunits were constructed.The original Duclos-negative patient homozygous for RHAG 316C>G, encoding Gln106Glu....
With 3 figures and 4 tables Abstract Alternaria blotch, caused by alternata apple pathotype, is an economically important disease of ( Malus × domestica Borkh.). Information on the resistance level cultivars to blotch key a strategy for integrated control disease, however, such information new apples recently released or scab‐resistant lacking. The aim this study was develop advanced technique, which allowed one reliable assessment evaluate levels various genotypes, especially apples....
During homologous recombination (HR)-mediated DNA double-strand break (DSB) repair in eukaryotes, an initial step is the creation of a 3′-single-stranded (ssDNA) overhang via resection 5′ end. Rad51 polymerizes on this ssDNA to search for sequence, and gapped sequence then repaired using undamaged strand as template. Recent studies eukaryotes indicate that DSB site promoted by cooperative action RecQ helicase family proteins: Bloom (BLM) mammals or Sgs1 yeast, exonuclease 1 (Exo1). However,...
Abstract Background Mammalian BLM helicase is involved in DNA replication, repair and homologous recombination (HR). These transactions are associated tightly with cell division important for maintaining genome stability. However, unlike mammals, higher plants restricted mainly to the meristem, thus maintenance at meristem critical. The counterpart of Arabidopsis ( AtRecQ4A ) has been identified its role HR response damage confirmed. function during replication stress not yet well...
Hippocampal CA 1 neurons are the most vulnerable to transient cerebral ischemia. However, mechanism has not been fully understood. The level of mRNA for cytochrome C oxidase (COX) subunit I (COX-I), which is encoded by mitochondrial (mt) DNA, progressively decreased in hippocampal gerbils from 3 h reperfusion after 3.5 min forebrain ischemia and completely disappeared at 7 days. activity COX protein also showed an early decrease cells was followed reduction COX-I DNA 2 succinic...