A5013426443- Plant Physiology and Cultivation Studies
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant tissue culture and regeneration
- Plant and animal studies
- Plant Virus Research Studies
- Horticultural and Viticultural Research
- Transgenic Plants and Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
- Plant nutrient uptake and metabolism
- Plant Disease Resistance and Genetics
- Plant biochemistry and biosynthesis
- Antioxidant Activity and Oxidative Stress
Plant & Food Research
2013-2023
University of Auckland
2000
Graduate School Experimental Plant Sciences
1999
Most published genome sequences are drafts, and most dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that not assigned to chromosomes, predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongyang' draft has 164 Mb of unassigned pseudo-chromosomes, omissions have been identified in the models.A second an A. (genotype Red5) was fully sequenced. This new resulted a 554.0 assembly with all...
Annualization of woody perennials has the potential to revolutionize breeding and production fruit crops rapidly improve horticultural species. Kiwifruit (Actinidia chinensis) is a recently domesticated crop with short history tremendous for improvement. Previously, multiple kiwifruit CENTRORADIALIS (CEN)-like genes have been identified as repressors flowering. In this study, CRISPR/Cas9- mediated manipulation enabled functional analysis CEN-like AcCEN4 AcCEN. Mutation these transformed...
FLOWERING LOCUS T (FT) and CENTRORADIALIS (CEN) homologs have been implicated in regulation of growth, determinacy flowering. The roles kiwifruit FT CEN were explored using a combination expression analysis, protein interactions, response to temperature high-chill low-chill cultivars ectopic Arabidopsis Actinidia. activity was opposite from that incorporated an interaction with D (FD)-like bZIP transcription factor. Accumulation transcript associated plant maturity particular stages leaf,...
The MADS-domain transcription factor SUPPRESSOR OF OVEREXPRESSION CONSTANS1 (SOC1) is one of the key integrators endogenous and environmental signals that promote flowering in annual species Arabidopsis thaliana. In deciduous woody perennial vine kiwifruit (Actinidia spp.), are integrated to regulate cycles growth dormancy. Accumulation chilling during winter required for dormancy break spring. order understand regulation kiwifruit, nine SOC1-like genes were identified characterized. All...
Summary Phosphatidylethanolamine‐binding protein (PEBP) genes regulate flowering and architecture in many plant species. Here, we study kiwifruit ( Actinidia chinensis , Ac ) PEBP with homology to BROTHER OF FT AND TFL1 BFT ). CRISPR‐Cas9 was used target AcBFT wild‐type fast‐flowering backgrounds. The editing construct designed preferentially AcBFT2 whose expression is elevated dormant buds. Acbft lines displayed an evergrowing phenotype increased branching, while control plants established...
Understanding the flowering process in apple (Malus × domestica Borkh.) is essential for developing methods to shorten breeding period and regulate fruit yield. It known that FLOWERING LOCUS T (FT) acts as a transmissible floral inducer Arabidopsis network system. To clarify molecular of two FT orthologues, MdFT1 MdFT2, we performed yeast two-hybrid screen identify proteins interact with MdFT1. We identified several transcription factors, including members TCP (TEOSINTE BRANCHED1, CYCLOIDEA...
SVP-like MADS domain transcription factors have been shown to regulate flowering time and both inflorescence flower development in annual plants, while having effects on growth cessation terminal bud formation perennial species. Previously, four SVP genes were described woody vine kiwifruit (Actinidia spp.), with possible distinct roles dormancy flowering. Kiwifruit SVP3 transcript was confined vegetative tissues acted as a repressor of it able rescue the Arabidopsis svp41 mutant. To...
Kiwifruit is a woody perennial horticultural crop, characterized by excessive vegetative vigor, prolonged juvenility, and low productivity. To understand the molecular factors controlling flowering winter dormancy, here we identify characterize kiwifruit PEBP (phosphatidylethanolamine-binding protein) gene family. Five CEN-like three BFT-like genes are differentially expressed act as functionally conserved floral repressors, while two MFT-like have no impact on time. FT-like expressed, with...
Temperate perennials require exposure to chilling temperatures resume growth in the following spring. Growth and dormancy cycles are controlled by complex genetic regulatory networks governed epigenetic mechanisms, but specific genes mechanisms remain poorly understood. To understand how seasonal changes regulate woody perennial vine kiwifruit (Ac, Actinidia chinensis), a transcriptome study of buds field conditions was performed. A MADS-box gene with homology Arabidopsis FLOWERING LOCUS C...
Kiwifruit (Actinidia chinensis) is a dioecious, long-living woody perennial vine. Reduced generation time and induction of hermaphroditism can accelerate crop improvement facilitate alternative farming for better food security in the face climate change. Previous studies identified that CENTRORADIALIS genes CEN CEN4 act to repress flowering, whilst male-specific Shy Girl (SyGl) gene with homology type-C cytokinin response regulators could gynoecium development model plants. Here we use...
Flower development in kiwifruit (Actinidia spp.) is initiated the first growing season, when undifferentiated primordia are established latent shoot buds. These can differentiate into flowers second after winter dormancy period and upon accumulation of adequate chilling. Kiwifruit an important horticultural crop, yet little known about molecular regulation flower development. To study development, nine MADS-box genes were identified functionally characterized. Protein sequence alignment,...
With the explosive numbers of sequences generated by next generation sequencing, demand for high throughput screening to understand gene function has grown. Plant viral vectors have been widely used as tools in down-regulating plant expression. However, can also express proteins a very efficient manner and, therefore, serve valuable tool characterizing and their functions metabolic pathways planta. In this study, we developed Gateway®-based vector cloning system from Narcissus Mosaic Virus...
The ZPT2-2 gene belongs to the EPF family in petunia (Petunia hybrida), which encodes proteins with TFIIIA-type zinc-finger DNA-binding motifs. To elucidate a possible function for ZPT2-2, we analyzed its pattern of expression relation different developmental and physiological stress signals. activity promoter was using firefly luciferase (LUC) reporter gene, allowing continuous measurements transgene planta. We show that ZPT2-2::LUC is active all plant tissues, but strongly modulated...
Kiwifruit (Actinidia chinensis) has three FLOWERING LOCUS T (FT) genes, AcFT, AcFT1, and AcFT2, with differential expression potentially divergent roles. AcFT was previously shown to be expressed in source leaves induced dormant buds by winter chilling. Here, we show that promotes flowering A. chinensis, despite a short sequence insertion not present other FT-like genes. A 3.5-kb promoter region contained all the regulatory elements required mediate vascular transgenic Arabidopsis thaliana...
Carotenoids are colorful lipophilic isoprenoids synthesized in all photosynthetic organisms which play roles plant growth and development provide numerous health benefits the human diet (precursor of Vitamin A). The commercially popular kiwifruits golden yellow-fleshed (
ABSTRACT Dioecy, the presence of male and female individuals, has evolved independently in multiple flowering plant lineages. Although theoretical models for evolution dioecy, such as “two-mutation” model, are well established, little is known about specific genes determining sex their evolutionary history. Kiwifruit, a major tree crop consumed worldwide, dioecious species. In kiwifruit, we had previously identified Y-encoded sex-determinant candidate gene acting suppressor feminization...
Trehalose metabolism and its intermediate trehalose-6-phosphate (T6P) are implicated in sensing signalling sucrose availability. Four class I TREHALOSE-6-PHOSPHATE SYNTHASE (TPS1) genes were identified kiwifruit, three of which have both the TPS phosphatase (TPP) domain, while fourth gene gives rise to a truncated transcript. The transcript with highest sequence homology Arabidopsis TPS1, designated TPS1.1a was most highly abundant TPS1 all examined kiwifruit tissues. An additional exon...