A5079250124- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Horticultural and Viticultural Research
- Plant Reproductive Biology
- Postharvest Quality and Shelf Life Management
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Plant Diversity and Evolution
- Biochemical and biochemical processes
- Plant-Microbe Interactions and Immunity
- Phytochemicals and Antioxidant Activities
- Lignin and Wood Chemistry
- Plant nutrient uptake and metabolism
- Plant Pathogens and Resistance
- Powdery Mildew Fungal Diseases
- Plant and animal studies
- Plant pathogens and resistance mechanisms
- Genetic and Environmental Crop Studies
- Plant Disease Resistance and Genetics
- AI and Big Data Applications
Plant & Food Research
2016-2025
Zhengzhou Fruit Research Institute
2018-2025
Chinese Academy of Agricultural Sciences
2018-2025
Henan Agricultural University
2025
Huazhong Agricultural University
2008
AgResearch
2002
Massey University
1994
Ethylene is the major effector of ripening in many fleshy fruits. In apples (Malus x domestica) addition ethylene causes a climacteric burst respiration, an increase aroma, and softening flesh. We have generated transgenic line 'Royal Gala' apple that produces no detectable levels using antisense ACC OXIDASE, resulting with ethylene-induced attributes. response to external these fruits undergo normal produced increasing concentrations ester, polypropanoid, terpene volatile compounds over 8-d...
Most published genome sequences are drafts, and most dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that not assigned to chromosomes, predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongyang' draft has 164 Mb of unassigned pseudo-chromosomes, omissions have been identified in the models.A second an A. (genotype Red5) was fully sequenced. This new resulted a 554.0 assembly with all...
Abstract Lignified stone cell content is a key factor used to evaluate fruit quality, influencing the economic value of pear (Pyrus pyrifolia) fruits. However, our understanding regulatory networks formation limited due complex secondary metabolic pathway. In this study, we combination co-expression network analysis, gene expression profiles, and transcriptome analysis in different cultivars with varied identify hub MYB gene, PbrMYB24. The relative PbrMYB24 flesh was significantly correlated...
Fruit development in higher plants normally requires pollination and fertilization to stimulate cell division of specific floral tissues. In some cases, parthenocarpic fruit proceeds without either or fertilization. Parthenocarpic seed has commercial value than seeded fruit. Several apple ( Malus domestica ) mutants (Rae Ime, Spencer Seedless Wellington Bloomless) are known produce only apetalous flowers that readily go on develop into Through genetics, a single recessive gene been...
Abstract We have characterized the tomato (Lycopersicon esculentum Mill.) MADS box gene TM29 that shared a high amino acid sequence homology to ArabidopsisSEP1, 2, and 3(SEPALLATA1, 3) genes. showed similar expression profiles toSEP1, with accumulation of mRNA in primordia all four whorls floral organs. In addition, was detected inflorescence vegetative meristems. To understandTM29 function, we produced transgenic plants which down-regulated by either cosuppression or antisense techniques....
Lignified stone cells substantially reduce fruit quality. Therefore, it is desirable to inhibit cell development using genetic technologies. However, the molecular mechanisms regulating lignification are poorly understood in cells. In this study, we have shown that microRNA (miR) miR397a regulates by inhibiting laccase (LAC) genes encode key lignin biosynthesis enzymes. Transient overexpression of PbrmiR397a, which Chinese pear (Pyrus bretschneideri), and simultaneous silencing three LAC...
Flowering plants utilize different floral structures to develop flesh tissue in fruits. Here we show that suppression of the homeologous SEPALLATA1/2-like genes MADS8 and MADS9 fleshy fruit apple (Malus x domestica) leads sepaloid petals greatly reduced flesh. Immunolabelling cell-wall epitopes differential staining showed developing hypanthium (from which develops) MADS8/9-suppressed flowers lacks a layer, remaining fully developed apples has considerably smaller cells. From these...
Abstract Background Genome structural variations (SVs) have been associated with key traits in a wide range of agronomically important species; however, SV profiles peach and their functional impacts remain largely unexplored. Results Here, we present an integrated map 202,273 SVs from 336 genomes. A substantial number selected during domestication improvement, which together affect 2268 genes. Genome-wide association studies 26 agronomic using these identify candidate causal variants. 9-bp...
The molecular genetic mechanisms underlying fruit size remain poorly understood in perennial crops, despite being an important agronomic trait. Here we show that the expression level of a microRNA gene (miRNA172) influences apple. A transposon insertional allele miRNA172 showing reduced associates with large apple breeding population, whereas over-expression transgenic significantly reduces size. was found to be co-located major quantitative trait locus, fixed cultivated apples and their...
Abstract Stone cells negatively affect fruit quality because of their firm and lignified cell walls, so are targets for reduction in pear breeding programmes. However, there is only limited knowledge the molecular mechanisms underlying formation stone cells. Here, we show that PbrMYB169, an R2R3 MYB transcription factor, Pyrus bretschneideri positively regulates lignification fruit. PbrMYB169 was shown to be co-expressed with lignin biosynthesis genes during development, this co-expression...
The plant hormone ethylene regulates ripening in climacteric fruits. phytohormone abscisic acid (ABA) affects biosynthesis, but whether influences ABA biosynthesis is unknown. To explore this possibility, we investigated the interactions between genes PpNCED2/3 and response transcription factor PpERF3 peach fruit. content increased during fruit maturation reached a peak at stage S4 III. increase was greatly inhibited by inhibitor 1-MCP, which also suppressed expression. shared similar...
In citrus, lignin overaccumulation in the juice sac results granulation and an unpleasant fruit texture taste. By integrating metabolic phenotyping transcriptomic analyses, we found 702 differentially expressed genes (DEGs), including 24 transcription factors (TFs), to be significantly correlated with content. CgMYB58 was further identified as a critical R2R3 MYB TF involved owing its high transcript levels Huanong Red-fleshed pummelo (HR, Citrus grandis) fruits. Transient expression of led...
Summary Allele‐specific expression (ASE) can lead to phenotypic diversity and evolution. However, the mechanisms regulating ASE are not well understood, particularly in woody perennial plants. In this study, we investigated genes apple cultivar ‘Royal Gala’ (RG). A high quality chromosome‐level genome was assembled using a homozygous tetra‐haploid RG plant, derived from anther cultures. Using RNA‐sequencing (RNA‐seq) data flower fruit tissues, identified 2091 genes. Compared with haploid of...
Abstract MicroRNA172 (miR172) plays a role in regulating diverse range of plant developmental processes, including flowering, fruit development and nodulation. However, its flavonoid biosynthesis is unclear. In this study, we show that transgenic apple plants over-expressing miR172 reduction red coloration anthocyanin accumulation various tissue types. This was consistent with decreased expression APETALA2 homolog MdAP2_1a (a target gene), MdMYB10, targets as demonstrated by both RNA-seq...
Although maturity date (MD) is an essential factor affecting fresh fruit marketing and has a pleiotropic effect on taste qualities, the underlying mechanisms remain largely unclear. In this study, we functionally characterized two adjacent NAM-ATAF1/2-CUC2 (NAC) transcription factors (TFs), PpNAC1 PpNAC5, both of which were associated with MD in peach. PpNAC5 found capable activating genes cell elongation, wall degradation ethylene biosynthesis, suggesting their regulatory roles enlargement...
Stone cells in fruits of pear (Pyrus pyrifolia) negatively influence fruit quality because their lignified cell walls impart a coarse and granular texture to the flesh.
Flower and fruit development are two key steps for plant reproduction. The ABCE model flower has been well established in species; however, the functions of genes crops less understood. In this work, we identified an EMS mutant named R27 woodland strawberry (Fragaria vesca), showing conversion petals, stamens, carpels to sepaloid organs a semidominant inheritance fashion. Mapping by sequencing revealed that class E gene homolog FveSEP3 (FvH4_4g23530) possessed causative mutation due G amino...
Flower and fruit colors are important agronomic traits. To date, there is no forward genetic evidence that the glutathione S-transferase (GST) gene responsible for white flower color in peach (Prunus persica). In this study, analysis indicated white-flower trait monogenetic, recessive to non-white allele, shows pleiotropic effects with non-white-flowered types. The locus underpinning was mapped onto chromosome 3 between 0.421951 3.227115 Mb by using bulked segregant conjunction whole-genome...
Abstract BABY BOOM (BBM) is a member of the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) family and its expression has been shown to improve herbaceous plant transformation regeneration. However, this improvement not clearly for tree species. This study demonstrated that efficiency transgenic apple (Malus domestica Borkh.) production was dramatically increased by ectopic MdBBM1 gene. “Royal Gala” plants were first transformed with CaMV35S-MdBBM1 construct (MBM) under kanamycin selection....