A5100324953- Plant Physiology and Cultivation Studies
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant and Fungal Species Descriptions
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Plant Diversity and Evolution
- Mesoporous Materials and Catalysis
- Zeolite Catalysis and Synthesis
- Circular RNAs in diseases
- Plant Virus Research Studies
- Plant and animal studies
- Ecology and Conservation Studies
- Polyamine Metabolism and Applications
- Laser Material Processing Techniques
- Receptor Mechanisms and Signaling
- Food Quality and Safety Studies
Wuhan Botanical Garden
2016-2025
Chinese Academy of Sciences
2015-2025
Shanghai Jiao Tong University
2005-2025
Renji Hospital
2025
Oil Crops Research Institute
2025
Kyushu University
2024
Institute of Coal Chemistry
2024
China University of Petroleum, East China
2024
Chinese PLA General Hospital
2020-2024
Cultural Relics Institute Hebei Province
2024
Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The contigs (2.25 gigabases (Gb)) cover approximately 94% whole genome, remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats tandem repeats. Comparisons with dog human showed that genome has lower divergence rate. assessment genes potentially underlying some its unique traits indicated bamboo diet might be more dependent on gut microbiome...
We report improved whole-genome shotgun sequences for the genomes of indica and japonica rice, both with multimegabase contiguity, or almost 1,000-fold improvement over drafts 2002. Tested against a nonredundant collection 19,079 full-length cDNAs, 97.7% genes are aligned, without fragmentation, to mapped super-scaffolds one other genome. introduce gene identification procedure plants that does not rely on similarity known remove erroneous predictions resulting from transposable elements....
Here we present the first diploid genome sequence of an Asian individual. The was sequenced to 36-fold average coverage using massively parallel sequencing technology. We aligned short reads onto NCBI human reference 99.97% coverage, and guided by genome, used uniquely mapped assemble a high-quality consensus for 92% individual’s genome. identified approximately 3 million single-nucleotide polymorphisms (SNPs) inside this region, which 13.6% were not in dbSNP database. Genotyping analysis...
Pyruvate kinase M2 isoform (PKM2) catalyzes the last step of glycolysis and plays an important role in tumor cell proliferation. Recent studies have reported that PKM2 also regulates apoptosis. However, mechanisms underlying such a remain elusive. Here we show translocates to mitochondria under oxidative stress. In mitochondria, interacts with phosphorylates Bcl2 at threonine (T) 69. This phosphorylation prevents binding Cul3-based E3 ligase subsequent degradation Bcl2. A chaperone protein,...
Most published genome sequences are drafts, and most dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that not assigned to chromosomes, predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongyang' draft has 164 Mb of unassigned pseudo-chromosomes, omissions have been identified in the models.A second an A. (genotype Red5) was fully sequenced. This new resulted a 554.0 assembly with all...
Plant-derived Vitamin C (l-ascorbic acid (AsA)) is crucial for human health and wellbeing thus increasing AsA content of interest to plant breeders. In plants GDP-l-galactose phosphorylase (GGP) a key biosynthetic control step here evidence presented two new transcriptional activators GGP. measurement, transcriptomics, transient expression, hormone application, gene editing, yeast 1/2-hybrid, electromobility shift assay (EMSA) methods were used identify positively regulating transcription...
Abstract Cold stress seriously affects plant development, resulting in heavy agricultural losses. L-ascorbic acid (AsA, vitamin C) is an antioxidant implicated abiotic tolerance and metabolism of reactive oxygen species (ROS). Understanding whether how cold elicits AsA biosynthesis to reduce oxidative damage important for developing cold-resistant plants. Here, we show that the accumulation response a common mechanism conserved across kingdom, from single-cell algae angiosperms. We...
Abstract Background Lactylation, a novel contributor to post-translational protein modifications, exhibits dysregulation across various tumors. Nevertheless, its intricate involvement in colorectal carcinoma, particularly for non-histone lactylation and intersection with metabolism immune evasion, remains enigmatic. Methods Employing immunohistochemistry on tissue microarray clinical information immunofluorescence cell lines, we investigated the presence of global association development...
Studies of a TAZ knockout mouse reveal novel function the transcriptional regulator TAZ, that is, as binding partner F-box protein β-Trcp. TAZ−/− mice develop polycystic kidney disease (PKD) and emphysema. The calcium-permeable cation channel polycystin 2 (PC2) is overexpressed in kidneys result decreased degradation via an SCFβ-Trcp E3 ubiquitin ligase pathway. Replacements serines phosphodegron motif prevent β-Trcp PC2 degradation. Coexpression cytoplasmic fragment 1 blocks PC2-TAZ...
Actinidia chinensis is an important economic plant belonging to the basal lineage of asterids. Availability a complete chloroplast genome sequence crucial understanding phylogenetic relationships among major lineages angiosperms and facilitates kiwifruit genetic improvement. We report here nucleotide sequences genomes for A. var deliciosa obtained through de novo assembly Illumina paired-end reads produced by total DNA sequencing. The size ranges from 155,446 157,557 bp, with inverted repeat...
Summary Kiwifruit is an important fruit crop; however, technologies for its functional genomic and molecular improvement are limited. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein (Cas) system has been successfully applied to genetic in many crops, but editing capability variable depending on the different combinations of synthetic guide RNA (sgRNA) Cas9 expression devices. Optimizing conditions use within a particular species therefore...
Kiwifruit (Actinidia spp.) is a climacteric fruit with high sensitivity to ethylene, influenced by multiple ethylene-responsive structural genes and transcription factors. However, the roles of other post-transcriptional regulators (e.g. miRNAs) necessary for ripening remain elusive. High-throughput sequencing sRNAome, degradome transcriptome methods were used identify further contributors control in kiwifruit (A. deliciosa cv 'Hayward'). Two NAM/ATAF/CUC domain factors (AdNAC6 AdNAC7), both...
Recurring epidemics of kiwifruit (Actinidia spp.) bleeding canker disease are caused by Pseudomonas syringae pv. actinidiae (Psa). In order to strengthen understanding population structure, phylogeography, and evolutionary dynamics, we isolated from cultivated wild across six provinces in China. Based on the analysis 80 sequenced Psa genomes, show that China is origin pandemic lineage but strain diversity confined just a single clade. contrast, Korea Japan harbor strains multiple clades....
Research studies have recently focused on circle RNAs (circRNAs) in relation to their regulatory functions animals. However, the systematic identification of circRNAs plants, especially non-model is limited. In addition, raw report prediction potential role plant response pathogen invasion currently available. We conducted from four materials originating three species belonging genus Actinidia under different situations using ribosomal RNA (rRNA) depleted RNA-Seq data. A total 3,582 were...
Kiwifruit is a recently domesticated horticultural fruit crop with substantial economic and nutritional value, especially because of the high content vitamin C in its fruit. In this study, we de novo assembled two telomere-to-telomere kiwifruit genomes from Actinidia chinensis var. 'Donghong' (DH) latifolia 'Kuoye' (KY), total lengths 608 327 852 640 561 626 bp for 29 chromosomes, respectively. With burst structural variants involving inversion, translocations, duplications within 8.39...
Summary Reticulate speciation caused by interspecific hybridization is now recognized as an important mechanism in the creation of biological diversity. However, depicting patterns phylogenetic networks for lineages that have undergone gene flow challenging. Here we sequenced 25 taxa representing natural diversity genus Actinidia with average mapping depth 26× on reference genome to reconstruct their reticulate history. We found evidence, including significant tree discordance, cytonuclear...
Angiosperm mitochondrial genomes (mitogenomes) are notable for their extreme diversity in both size and structure. However, our current understanding of this is limited, the underlying mechanism contributing to remains unclear. Here, we completely assembled compared mitogenomes three kiwifruit (Actinidia) species, which represent an early divergent lineage asterids. We found conserved gene content fewer genomic repeats, particularly large repeats (>1 kb), mitogenomes. sequence transfers such...
Polyploidization is hypothesized to improve the freezing resistance of plants in cold regions. However, adaptive strategies and key physiological mechanisms involved resistant ability polyploids remain unclear. In Actinidia chinensis (kiwifruits), tetraploids hexaploids occupy higher altitude habitats with colder climates than diploids, providing a study system investigate responsible for differentiation between cytotypes. We characterized environmental conditions their natural distribution...
The purple coloration of pepper leaves arises from the accumulation anthocyanin. Three regulatory and 12 structural genes have been characterized for their involvement in anthocyanin biosynthesis. Examination abundance these showed that majority them differed between pigmented line Z1 non-pigmented A3. Silencing R2R3-MYB transcription factor CaMYB resulted loss accumulation. Moreover, expression multiple was altered silenced leaves. MYC significantly lower CaMYB-silenced leaves, whereas WD40...
Abstract An outbreak of kiwifruit bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) beginning in 2008 disaster to the industry. However mechanisms interaction between and Psa are unknown. Long noncoding RNAs (lncRNAs) known regulate many biological processes, but comprehensive repertoires lncRNAs their effects on Here, based in-depth transcriptomic analysis four materials at three stages infection with Psa, we identified 14,845 transcripts from 12,280 loci as...