- Plant Physiology and Cultivation Studies
- Greenhouse Technology and Climate Control
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Advanced Combustion Engine Technologies
- Plant Molecular Biology Research
- Combustion and flame dynamics
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- RNA regulation and disease
- Genomics and Phylogenetic Studies
- Postharvest Quality and Shelf Life Management
- Light effects on plants
- Biochemical and biochemical processes
- Seed Germination and Physiology
- Advanced Aircraft Design and Technologies
- Plant and Fungal Interactions Research
- Phytase and its Applications
- Plant Disease Management Techniques
Chinese Academy of Sciences
2017-2024
Wuhan Botanical Garden
2019-2024
Lushan Botanical Garden
2022
South China Botanical Garden
2017-2018
Northwestern Polytechnical University
2018
Beijing Botanical Garden
2017-2018
University of Chinese Academy of Sciences
2017-2018
Yancheng Institute of Technology
2015
Summary Kiwifruit is an important fruit crop; however, technologies for its functional genomic and molecular improvement are limited. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein (Cas) system has been successfully applied to genetic in many crops, but editing capability variable depending on the different combinations of synthetic guide RNA (sgRNA) Cas9 expression devices. Optimizing conditions use within a particular species therefore...
Research studies have recently focused on circle RNAs (circRNAs) in relation to their regulatory functions animals. However, the systematic identification of circRNAs plants, especially non-model is limited. In addition, raw report prediction potential role plant response pathogen invasion currently available. We conducted from four materials originating three species belonging genus Actinidia under different situations using ribosomal RNA (rRNA) depleted RNA-Seq data. A total 3,582 were...
Post-harvest rot causes enormous economic loss to the global kiwifruit industry. Currently, there are no effective fungicides combat disease. It is unclear whether silver nanoparticles (AgNPs) in controlling post-harvest and, if so, what underlying antifungal mechanism is. Our results indicated that 75 ppm AgNPs effectively inhibited mycelial growth and spore germination of four pathogens: Alternaria alternata, Pestalotiopsis microspora, Diaporthe actinidiae, Botryosphaeria dothidea....
Summary Reticulate speciation caused by interspecific hybridization is now recognized as an important mechanism in the creation of biological diversity. However, depicting patterns phylogenetic networks for lineages that have undergone gene flow challenging. Here we sequenced 25 taxa representing natural diversity genus Actinidia with average mapping depth 26× on reference genome to reconstruct their reticulate history. We found evidence, including significant tree discordance, cytonuclear...
Angiosperm mitochondrial genomes (mitogenomes) are notable for their extreme diversity in both size and structure. However, our current understanding of this is limited, the underlying mechanism contributing to remains unclear. Here, we completely assembled compared mitogenomes three kiwifruit (Actinidia) species, which represent an early divergent lineage asterids. We found conserved gene content fewer genomic repeats, particularly large repeats (>1 kb), mitogenomes. sequence transfers such...
The TIFY gene family is a group of plant-specific transcription factors involved in regulation plant growth and development variety stress responses. However, the has not yet been well characterized kiwifruit, popular fruit with important nutritional economic value.A total 27 21 genes were identified genomes Actinidia eriantha A. chinensis, respectively. Phylogenetic analyses showed that kiwifruit could be classified into four major groups, JAZ, ZML, PPD, JAZ further clustered six subgroups...
Abstract An outbreak of kiwifruit bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) beginning in 2008 disaster to the industry. However mechanisms interaction between and Psa are unknown. Long noncoding RNAs (lncRNAs) known regulate many biological processes, but comprehensive repertoires lncRNAs their effects on Here, based in-depth transcriptomic analysis four materials at three stages infection with Psa, we identified 14,845 transcripts from 12,280 loci as...
The current kiwifruit industry is mainly based on the cultivars derived from species Actinidia chinensis (Ac) which may bring risks such as canker disease. Introgression of desired traits wild relatives an important method for improving cultivars. eriantha (Ae) a particularly taxon used hybridization or introgressive breeding new because its valued species-specific traits. Here, we assembled chromosome-scale high-quality genome Ae sample was directly collected populations. Our analysis...
Light-harvesting chlorophyll a/b-binding (LHC) protein is a superfamily that plays vital role in photosynthesis. However, the reported knowledge of LHCs kiwifruit inadequate and poorly understood. In this study, we identified 42 45 LHC genes Actinidia chinensis (Ac) A. eriantha (Ae) genomes. Phylogenetic analysis showed both species were grouped into four subfamilies (Lhc, Lil, PsbS, FCII). Expression profiles qRT-PCR results revealed expression levels closely related to light, temperature...
Postharvest soft rot of kiwifruit has resulted in substantial market losses, yet there were few antagonistic yeasts reported to control the disease. This study screened 1113 yeast strains for potential caused by Botryosphaeria dothidea and Diaporthe actinidiae, strain 37 was selected evaluate efficacy mechanisms, which identified as Meyerozyma guilliermondii via molecular biological identification. Our results showed that M. effectively reduced pathogen spore germination rate 28.52% decay...
The STAY-GREEN (SGR) proteins play an important role in chlorophyll (Chl) degradation and are closely related to plant photosynthesis. However, the availability of inadequate studies on SGR motivated us conduct a comprehensive study identification functional dissection superfamily members kiwifruit. Here, we identified five genes for each kiwifruit species [Actinidia chinensis (Ac) Actinidia eriantha (Ae)]. phylogenetic analysis showed that were divided into two subfamilies subfamily SGRL...
Adenine base editor containing TadA8e (ABE8e) has been reported in rice. However, the application of ABE8e other plant species not described, and comparison between ABE7.10, which is widely used plants, also poorly studied. Here, we developed with polycistronic tRNA-gRNA expression cassette (PTG-ABE8e) PTG-ABE7.10 compared their A-to-G editing efficiencies using both transient stable transformation allotetraploid Nicotiana benthamiana. We found that efficiency PTG-ABE8e was significantly...
High temperatures have a significant impact on plant growth and metabolism. In recent years, the fruit industry has faced serious threat due to high-temperature stress plants caused by global warming. present study, we explored molecular regulatory mechanisms that contribute tolerance in kiwifruit. A total of 36
The WUSCHEL (WUS)-related homeobox (WOX) gene family is a class of plant-specific transcriptional factors and plays crucial role in forming the shoot apical meristem embryonic development, stem cell maintenance, various other developmental processes. However, systematic identification characterization kiwifruit WOX have not been studied. This study identified 17 10 genes A. chinensis (Ac) eriantha (Ae) genomes, respectively. Phylogenetic analysis classified from two species into three...
Growth-regulating factors (GRFs) encode plant-specific transcription that play a vital role in regulation of plant growth, development, and stress response. Although GRFs have been identified various plants, there is no reported work available Actinidia (commonly known as kiwifruit) so far. In the present study, we 22 GRF genes on A. chinensis (hereafter referred to Ac, are AcGRF) distributed 17 chromosomes one contig, 26 eriantha Ae, AeGRF) 21 chromosomes. Phylogenetic analysis showed...
Elaeagnus L. is found in wild or grown as ornamental plants and increasingly regarded underutilized berry shrubs by breeders. This genus has cosmopolitan distribution with various species widely distributed China, Europe, the United States, Canada. Interspecific hybrids, which have been reported several times, attracted intense interest from plant breeders attempting to develop a fruit crop of Elaeagnus. Orthogonal projections latent structures discriminant analysis (OPLS-DA) powerful...
Abstract To predict the pollutant emissions and lean blowout, chemical reactor network (CRN) model is applied to modern aircraft engine combustion chamber. In this study, CRN which represent major features of aerodynamics in chamber set up based on OpenFOAM simulation results. The boundary initial conditions used for derive from operating modes typical cycle. A 21 species 30 steps mechanism kerosene employed method. levels are obtained under four ICAO power settings idle, approach climb take...