Rapeseed (Brassica napus), is an important source of edible oil, animal fodder, vegetables, condiments and biodiesel, plays a significant role in securing oil production worldwide. However, countries with comparatively low levels agricultural mechanization, such as China, increasing costs labor inputs are decreasing rapeseed profitability, hence the area under cultivation. If value crops not further increased, growing will continue to decrease, potentially jeopardizing production. Therefore,...

Sucrose synthase (SUS) is a common sugar-base transfer enzyme in plants, and sucrose phosphate (SPS) one of the major enzymes higher plants that regulates synthesis. However, information SPS SUS gene families Actinidia, as well their evolutionary functional properties, limited. According to proteins conserved domain Arabidopsis thaliana, we found 6 genes from A. chinensis (cultivar: 'Hongyang'), 3 eriantha 'White'). The novel CDC50 domains were discovered on AcSUS2, all members family...

The TIFY gene family is a group of plant-specific transcription factors involved in regulation plant growth and development variety stress responses. However, the has not yet been well characterized kiwifruit, popular fruit with important nutritional economic value.A total 27 21 genes were identified genomes Actinidia eriantha A. chinensis, respectively. Phylogenetic analyses showed that kiwifruit could be classified into four major groups, JAZ, ZML, PPD, JAZ further clustered six subgroups...

Kiwifruit is a unique berry fruit which rich in various nutrients and antioxidant substances. As typical climacteric fruit, the after-ripening of kiwifruit accompanied by rapid decline firmness, greatly shortens shelf life. Here, new storage method, on-vine ripening, was used to investigate changes quality activity cultivar, Actinidia eriantha 'Ganlv 1'. The results indicated that delayed harvesting 14–21 days (for transportation) or 35–42 ready-to-eat) suitable for A. 1' also improves...

Abstract Background Actinidia eriantha is a precious material to study the metabolism and regulation of ascorbic acid (AsA) because its high AsA content. Although pathway biosynthesis in kiwifruit has been identified, mechanism still unclear. The purpose this experiment reveal metabolic characteristics A. ‘Ganmi 6’ from molecular level, lay theoretical foundation for research on genetic improvement quality. Results We found that reached accumulation peak at S7 (110 DAF) during process fruit...

Actinidia eriantha is one of the most important kiwifruit species in Actinidia. The relative high accumulation organic acids fruit A. an unfavorable factor for organoleptic quality. To identify key metabolic enzymes and genes involved during development, physiological, biochemical, molecular experiments were conducted dynamic samples a new cultivar, 'Ganlv 1'.The contents citric acid malic increased greatly while quinic content decreased obviously. Significant positive correlations observed...

Abstract Background NAC transcription factors (TFs) are plant-specific proteins encoded by a large gene family. They play important roles in diverse biological processes, such as plant growth and development, leaf senescence, responses to biotic or abiotic stresses. Functions of number TFs have been identified mainly model plants. However, very few studies on conducted the fruit tree kiwifruit. Results Genome-wide genes were their phylogeny, genomic structure, chromosomal location, synteny...

The widespread ascorbic acid (AsA) plays a vital role in plant development and abiotic stress tolerance, but AsA concentration varies greatly among different plants. GDP-D-mannose epimerase (GME), which catalyzes to GDP-L-galactose or GDP-L-gulose, is key enzyme biosynthesis pathway. Functions expression patterns of GME have been well studied previous works, however, little information known about the evolutionary gene. In this study, gene structure, corresponding conserved protein motifs...

Studies on organic acid metabolism have been mainly concentrated the fruit, whereas, few focused mechanism of high acids content in fruit Actinidia eriantha. Fruits ‘Ganmi 6’ harvested at eleven developmental periods were used as materials. The components and determined by high-performance liquid chromatography (HPLC) system, activities related enzyme detected, gene expression levels measured quantitative real-time PCR (qRT-PCR). Components ascorbic (AsA) eight kinds detected. These results...

Actinidia eriantha is one of the species with particularly high ascorbic acid (AsA) content in kiwifruit. However, molecular mechanism AsA rich fruit remain unclear. In order to reveal A. eriantha, this study constructed a regulatory network related metabolism by combining genomics, metabolomics and transcriptomics. Here, we assembled high-quality genome 'Ganlv 1' only five gaps remained. The assembly comprising 29 pseudochromosomes total size 615.95 Mb, contig N50 20.35 Mb. Among them, 24...

Ascorbic acid is a potent antioxidant and crucial nutrient for plants animals. The accumulation of ascorbic in controlled by its biosynthesis, recycling, degradation. Monodehydroascorbate reductase deeply involved the cycle; however, mechanism monodehydroascorbate genes regulating kiwifruit remains unclear. Here, we identified seven genome (Actinidia eriantha) they were designated as AeMDHAR1 to AeMDHAR7, following their identifiers. We found that relative expression level AeMDHAR3 fruit...

Kiwifruit (Actinidia eriantha) is a peculiar berry resource in China, and the maturation period generally late. Fortunately, we found an early mature A. eriantha germplasm. In order to explore formation mechanism of its trait, determined main carbohydrate endogenous hormone content fruit, used off-target metabolomics transcriptomics identify key regulatory metabolites genes. We that germplasm had faster starch conversion rate higher sucrose, glucose, fructose when harvested, while with lower...

Abstract Ascorbic acid (AsA) is a widespread antioxidant in living organisms, and plays essential roles the growth development of animals plants as well response to abiotic stress tolerance. The GDP-L-galactose phosphorylase (GGP) key regulatory gene plant AsA biosynthesis that can regulate concentration at transcriptional translational levels. function regulation mechanisms GGP have been understood; however, molecular evolutionary patterns remain unclear. In this study, total 149 homologous...

‘Ganlv 1’ is a new cultivar of Actinidia eriantha selected from the wild natural population, which has advantages moderate taste, high yield, easy peeling and ascorbic acid (AsA) content. In this study, was used to explore changes in fruit quality, soluble sugar components, sucrose metabolism-related enzymes activities enzyme genes’ expression during fruit’s development. The results showed that, except for AsA, quality index growth development basically exhibited same trend. shape different...

According to the investigation of wild Actinidia eriantha in Jiangxi province China, we found that soluble solids content fruit was lower than edible standard (14%). However, a high-sugar type A. line (code 'MM24', test material) during investigative process at Nancheng county (E 116° 48', N 26° 23', 845 m). We sheared its scions asexual reproduction Fengxin County (rootstock deliciosa 'Miliang 1' with 7 years old) and same time DUS (Distinctness, Uniformity Stability) also carried out....