A5063394323- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Light effects on plants
- Polysaccharides and Plant Cell Walls
- Bone Tissue Engineering Materials
- Silicon and Solar Cell Technologies
- Plant Stress Responses and Tolerance
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Biofuel production and bioconversion
- Knee injuries and reconstruction techniques
- Ubiquitin and proteasome pathways
- GABA and Rice Research
- Plant responses to water stress
- Antioxidant Activity and Oxidative Stress
- Titanium Alloys Microstructure and Properties
- Semiconductor materials and interfaces
- Thin-Film Transistor Technologies
- Enzyme Production and Characterization
- Dental Implant Techniques and Outcomes
- Rice Cultivation and Yield Improvement
- Shoulder Injury and Treatment
National Agriculture and Food Research Organization
2018-2024
Institute of Crop Science
2018-2024
Institute of Agrobiological Sciences
2005-2018
Nagoya University
1998-2017
University of Minnesota
2009-2012
Plant (United States)
2011
The University of Tokyo
2004-2011
University of Tsukuba
2011
National Archives and Records Administration
2002
RIKEN
1999
Abstract To enhance our understanding of GA metabolism in rice (Oryza sativa), we intensively screened and identified 29 candidate genes encoding the following metabolic enzymes using all available DNA databases: ent-copalyl diphosphate synthase (CPS), ent-kaurene (KS), oxidase (KO), ent-kaurenoic acid (KAO), 20-oxidase (GA20ox), 3-oxidase (GA3ox), 2-oxidase (GA2ox). In contrast to Arabidopsis genome, multiple CPS-like, KS-like, KO-like were most which are contiguously arranged. We also 18...
Gibberellin (GA) regulates growth and development in plants. We isolated characterized a rice GA-insensitive dwarf mutant,gid2. The GID2 gene encodes putative F-box protein, which interacted with the Skp1 homolog yeast two-hybrid assay. In gid2, repressor for GA signaling, SLR1, was highly accumulated phosphorylated form increased its concentration, whereas SLR1 rapidly degraded by through ubiquitination wild type. conclude that is positive regulator of signaling regulated degradation...
The slender rice1 mutant (slr1) shows a constitutive gibberellin (GA) response phenotype. To investigate the mode of action SLR1, we generated transgenic rice expressing fusion protein consisting SLR1 and green fluorescent (SLR1-GFP) analyzed phenotype transformants subcellular localization GFP in vivo. SLR1-GFP worked nuclei to repress GA signaling pathway; its overproduction caused dwarf Application GA3 overproducers induced actions such as shoot elongation, downregulation 20-oxidase...
Auxin regulates most aspects of plant growth and development. The hormone is perceived by the TIR1/AFB family F-box proteins acting in concert with Aux/IAA transcriptional repressors. Arabidopsis plants that lack members are auxin resistant display a variety defects. However, little known about functional differences between individual family. Phylogenetic studies reveal conserved across land lineages fall into four clades. Three these subgroups emerged before separation angiosperms...
Summary The plant hormone auxin controls numerous aspects of growth and development by regulating the expression hundreds genes. SMALL AUXIN UP RNA ( SAUR ) genes comprise largest family auxin‐responsive genes, but their function is unknown. Although prior studies have correlated some with auxin‐mediated cell expansion, genetic evidence implicating SAURs in expansion has not been reported. Arabidopsis SAUR19 , SAUR20 SAUR21 SAUR22 SAUR23 SAUR24 SAUR19–24 encode a subgroup closely related...
Abstract A major catabolic pathway for gibberellin (GA) is initiated by 2β-hydroxylation, a reaction catalyzed GA 2-oxidase. We have isolated and characterized cDNA, designated Oryza sativa 2-oxidase 1 (OsGA2ox1) from rice (Oryza L. cv Nipponbare) that encodes The encoded protein, produced heterologous expression inEscherichia coli, converted GA1, GA4, GA9, GA20, GA44 to the corresponding 2β-hydroxylated products GA8, GA34, GA51, GA29, GA98, respectively. Ectopic of theOsGA2ox1 cDNA in...
GAMYB was first isolated as a positive transcriptional regulator of gibberellin (GA)-dependent alpha-amylase expression in barley aleurone cells, and its molecular biochemical properties have been well characterized. However, the role elsewhere plant is not understood. To investigate function outside we loss-of-function mutants from panel rice produced by insertion retrotransposon, Tos17. Through PCR screening using primers for (OsGAMYB) Tos17, three independent mutant alleles that contained...
We have cloned two gibberellin (GA) 3β-hydroxylase genes, OsGA3ox1 and OsGA3ox2 , from rice by screening a genomic library with DNA fragment obtained PCR using degenerate primers. used full-scan GC-MS Kovats retention indices to show function for the encoded recombinant fusion proteins. Both proteins activity steps GA 20 1 5 3 44 38 9 4 . In addition, indirect evidence suggests that protein also has 2,3-desaturase activity, which catalyzes 2,3-dehydro-GA (2,3-dehydro ), 2β-hydroxylase 8 34...
A rice semi-dwarf variety, IR8, known as “miracle rice” enabled dramatic increases production and its widespread adoption averted predicted food shortages in Asia during the 1960s to 1990s. This remarkable achievement was referred “green revolution”. The short stature of IR8 derived from gene, sd1, sd1 gene contributed significantly In this paper, we described physiological, molecular genetic biochemical characterization gene. mutant contained lower gibberellin (GA) levels than wild-type...
Summary The phytohormone gibberellin (GA) controls growth and development in plants. Previously, we identified a rice F‐box protein, gibberellin‐insensitive dwarf2 (GID2), which is essential for GA‐mediated DELLA protein degradation. In this study, analyzed the biological molecular properties of GID2. Expression GID2 preferentially occurred organs actively synthesizing GA. Domain analysis revealed that C‐terminal regions were function, but not N‐terminal region. Yeast two‐hybrid assay...
Summary To identify where gibberellin (GA) biosynthesis and signaling occur, we analyzed the expression of four genes involved in GA biosynthesis, 20‐oxidase1 20‐oxidase2 ( OsGA20ox1 OsGA20ox2 ), 3‐oxidase1 3‐oxidase2 OsGA3ox1 OsGA3ox2 two signaling, namely, gene encoding α‐subunit heterotrimeric GTP‐binding protein Gα SLENDER RICE1 SLR1 which encodes a repressor signaling. At vegetative stage, , was observed rapidly elongating or dividing organs tissues, whereas could not be detected....
Abstract The circadian clock controls physiological traits such as flowering time, photosynthesis, and growth in plants under laboratory conditions. Under natural field conditions, however, little is known about the significance of plants. By time-course transcriptome analyses rice (Oryza sativa) leaves, using a newly isolated clock–related mutant carrying null mutation Os-GIGANTEA (Os-GI), we show here that Os-GI controlled 75% (false discovery rate = 0.05) genes among 27,201 tested was...
Abstract We recently isolated two genes (OsGA3ox1 andOsGA3ox2) from rice (Oryza sativa) encoding 3β-hydroxylase, which catalyzes the final step of active gibberellin (GA) biosynthesis (H. Itoh, M. Ueguchi-Tanaka, N. Sentoku, H. Kitano, Matsuoka, Kobayashi [2001] Proc Natl Acad Sci USA 98: 8909–8914). Using these cloned cDNAs, we analyzed temporal and spatial expression patterns 3β-hydroxylase also an α-amylase gene (RAmy1A) during seed germination to investigate relationship between GA...
Differential distribution of the plant hormone auxin within tissues mediates a variety developmental processes. Cellular levels are determined by metabolic processes including synthesis, degradation, and (de)conjugation, as well transport across plasma membrane. Whereas free auxins such naturally occurring indole-3-acetic acid (IAA) is characterized, little known about precursors metabolites. Here, we identify mutation in ABCG37 gene Arabidopsis that causes polar inhibitor sensitive1 (pis1)...
Summary The rice SLR1 ( SLENDER RICE 1 ) gene encodes a DELLA protein that belongs to subfamily of the GRAS superfamily and functions as repressor gibberellin (GA) signaling. Based on constitutive GA response phenotype slr1 mutants, has been thought be sole DELLA‐type suppressing signals in rice. However, genome databases we identified two sequences homologous : SLR1‐like1 ‐ 2 SLRL1 ). SLRL2 contain regions with high similarity C‐terminal conserved domains SLR1, but lack N‐terminal region...
Phytochromes mediate the photoperiodic control of flowering in rice (Oryza sativa), a short-day plant. Recent molecular genetics studies have revealed genetic network that enables critical daylength response florigen gene expression. Analyses using phytochrome chromophore-deficient mutant, photoperiod sensitivity5, so far within this network, phytochromes are required for expression Grain number, plant height and heading date7 (Ghd7), floral repressor rice. There three genes rice, but roles...
Abstract Because plant reproductive development occurs only in adult plants, the juvenile-to-adult phase change is an indispensable part of life cycle. We identified two allelic mutants, peter pan syndrome-1 (pps-1) and pps-2, that prolong juvenile rice (Oryza sativa) showed PPS ortholog Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENIC1. The pps-1 mutant exhibits delayed expression miR156 miR172 suppression GA biosynthetic genes, reducing GA3 content this mutant. In spite its prolonged...
Summary Ectopic expression of the homeobox gene, NTH15 ( Nicotiana tabacum 15) in transgenic tobacco leads to abnormal leaf and flower morphology, accompanied by a decrease content active gibberellin, GA 1 . Quantitative analysis intermediates biosynthetic pathway revealed that step from 19 20 was blocked plants overexpressing To investigate relationship between genes involved biosynthesis, we isolated three cDNA clones encoding two types 20‐oxidase 3β‐hydroxylase. RNA gel blot one gene...