A5058758057- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Rice Cultivation and Yield Improvement
- GABA and Rice Research
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Aluminum toxicity and tolerance in plants and animals
- Seed Germination and Physiology
- Mitochondrial Function and Pathology
- Plant tissue culture and regeneration
- Plant Micronutrient Interactions and Effects
- Wheat and Barley Genetics and Pathology
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Gene expression and cancer classification
- Photoreceptor and optogenetics research
- Crop Yield and Soil Fertility
National Agriculture and Food Research Organization
2018-2025
Institute of Crop Science
2018-2023
Institute of Agrobiological Sciences
2008-2019
Institute of Crop Sciences
2018
Kyoto University
2012-2017
Hirosaki National Hospital
2017
University of Münster
2017
Mitsubishi Group (Japan)
2010-2012
The University of Tokyo
2012
University of Kochi
2009
Five retrotransposon families of rice (Tos1-Tos5) have been reported previously. Here we report 15 new (Tos6-Tos20). In contrast to yeast and Drosophila retrotransposons, all the retrotransposons examined appear inactive (or almost inactive) under normal growth conditions. Three (Tos10, Tos17, Tos19) are activated tissue culture The most active one, was studied in detail. copy number Tos17 increased with prolonged period. plants regenerated from cultures, including transgenic plants, 5 30...
Metals like manganese (Mn) and iron (Fe) are essential for metabolism, while cadmium (Cd) is toxic virtually all living organisms. Understanding the transport of these metals important breeding better crops. We have identified that OsNRAMP5 contributes to Mn, Fe Cd in rice. expression was restricted roots epidermis, exodermis outer layers cortex as well tissues around xylem. localized plasma membrane complemented growth yeast strains defective transport. RNAi (OsNRAMP5i) plants accumulated...
A wide range of resources on gene expression profiling enhance various strategies in plant molecular biology particularly characterization function. We have updated our profile database, RiceXPro (http://ricexpro.dna.affrc.go.jp/), to provide more comprehensive information the transcriptome rice encompassing entire growth cycle and experimental conditions. The profiles are currently grouped into three categories, namely, 'field/development' with 572 data corresponding 12 sets, 'plant...
Seed dormancy provides a strategy for flowering plants to survive adverse natural conditions. It is also an important agronomic trait affecting grain yield, quality, and processing performance. We cloned rice quantitative locus, Sdr4 , which contributes substantially differences in seed between japonica (Nipponbare) indica (Kasalath) cultivars. expression positively regulated by OsVP1 global regulator of maturation, turn regulates potential regulators represses the postgerminative genes,...
Tolerance to abiotic stress is an important agronomic trait in crops and controlled by many genes, which are called quantitative loci (QTLs). Identification of these QTLs will contribute not only the understanding plant biology but also for breeding, achieve stable crop production around world. Previously, we mapped three controlling low-temperature tolerance at germination stage (called germinability). To understand molecular basis one QTLs, qLTG3-1 (quantitative locus germinability on...
Elucidating the function of all predicted genes in rice remains as ultimate goal cereal genomics order to ensure development improved varieties that will sustain an expanding world population. We constructed a gene expression database (RiceXPro, URL: http://ricexpro.dna.affrc.go.jp/ ) provide overview transcriptional changes throughout growth plant field. RiceXPro contains two data sets corresponding spatiotemporal profiles various organs and tissues, continuous leaf from transplanting...
Floret fertility is a key determinant of the number grains per inflorescence in cereals. During evolution wheat (Triticum sp.), floret has increased, such that current bread aestivum) cultivars set three to five spikelet. However, little known regarding genetic basis fertility. The locus Grain Number Increase 1 (GNI1) shown here be an important contributor GNI1 evolved Triticeae through gene duplication. gene, which encodes homeodomain leucine zipper class I (HD-Zip I) transcription factor,...
Significance Genetically improving the root system architectures of plants is an effective strategy for developing climate-resilient crops. In this study, we revealed that a cloned rice quantitative trait locus associated with growth angle, qSOR1 , DRO1 homolog involved in gravitropic responses. The loss-of-function allele qsor1 resulted roots developed on soil surface and enabled to avoid reducing stress found saline paddy soils and, consequently, increased yields. We show homologs could be...
Abstract We have isolated a new recessive dwarf mutant of rice (Oryza sativa L. cv Nipponbare). Under normal growth conditions, the has very short leaf sheaths; short, curled, and frizzled blades; few tillers; is sterile. Longitudinal sections sheaths revealed that cell length along longitudinal axis reduced, which explains sheaths. Transverse blades enlargement motor cells dorsal-ventral axis, curled blades. In addition, number crown roots was smaller branch weaker than those in wild-type...
Improvement of leaf photosynthesis is an important strategy for greater crop productivity. Here we show that the quantitative trait locus GPS (GREEN FOR PHOTOSYNTHESIS) in rice (Oryza sativa L.) controls rate by regulating carboxylation efficiency. Map-based cloning revealed identical to NAL1 (NARROW LEAF1), a gene previously reported control lateral growth. The high-photosynthesis allele was found be partial loss-of-function NAL1. This increased mesophyll cell number between vascular...
Summary Oryza sativa (rice) flowers in response to photoperiod, and is a facultative short‐day (SD) plant. Under SD conditions, flowering promoted through the activation of FT‐like genes (rice florigens) by Heading date 1 ( Hd1 , rice CONSTANS homolog) Early heading Ehd1 with no ortholog Arabidopsis genome). On other hand, under long‐day (LD) delayed repressive function on downregulation repressor Ghd7 – unique pathway rice. We report here that an early 3 ehd3 ) mutant flowered later than...
Similarity of gene expression across a wide range biological conditions can be efficiently used in characterization function. We have constructed rice coexpression database, RiceFREND (http://ricefrend.dna.affrc.go.jp/), to identify modules with similar profiles and provide platform for more accurate prediction functions. Coexpression analysis 27 201 genes was performed against 815 microarray data derived from profiling various organs tissues at different developmental stages, mature...
The improvement of resistance to blast, a devastating fungal disease rice, would support the sustainable production one world's staple foods, yet identification genes for durable in rice is challenge owing their complicated genetic control. Here we show that map-based cloning Pi35 identifies multiple functional polymorphisms allow effective control and allelic Pish, which mediates race-specific blast encodes protein containing nucleotide-binding site (NBS) leucine-rich repeats (LRRs)....
Abstract Background Plant growth depends on synergistic interactions between internal and external signals, yield potential of crops is a manifestation how these complex factors interact, particularly at critical stages development. As an initial step towards developing systems-level understanding the biological processes underlying expression overall agronomic in cereal crops, high-resolution transcriptome analysis rice was conducted throughout life cycle grown under natural field...
Abstract Plasticity of root growth in response to environmental cues and stresses is a fundamental characteristic land plants. However, the molecular basis underlying regulation under stressful conditions poorly understood. Here, we report that rice nuclear factor, RICE SALT SENSITIVE3 (RSS3), regulates cell elongation during adaptation salinity. Loss function RSS3 only moderately inhibits normal conditions, but it provokes spontaneous swelling, accompanied by severe inhibition, saline...
Summary The tobacco Tto1 is one of the few active LTR‐retrotransposons plants, and its transposition activated by tissue culture primarily regulated at transcriptional level. expression RNA can also be various stresses, including viral infection, wounding, treatment with jasmonate, a signal molecule plant defence responses. It shown here that LTR promoter responsible for high level in cultured tissues transgenic plants. We demonstrate 13‐bp repeated motif (TGGTAGGTGAGAT) functions as...
Transposition of the tobacco retrotransposon Tto1 is regulated mainly by transcription from long terminal repeat (LTR). Functional analysis LTR showed that 13-bp motif a cis-regulatory element involved in activation tissue culture, wounding, and treatment with elicitors. The contains conserved (L box) has been implicated expression phenylpropanoid synthetic genes response to defense-related stresses. To gain further insight into regulatory mechanism genes, cDNAs encoding four different...
Summary The chloroplast NADH dehydrogenase‐like (NDH) complex mediates cyclic electron transport and chloro‐respiration consists of five sub‐omplexes, which in angiosperms further associate with photosystem I (PSI) to form a super‐complex. In Marchantia polymorpha , 11 plastid‐encoded subunits all the nuclear‐encoded A, B, membrane ferredoxin‐binding sub‐complexes are conserved. However, it is unlikely that genome this liverwort encodes Lhca5 Lhca6, both mediate NDH–PSI super‐complex...
Previously, we reported that OsNRAMP5 functions as a manganese, iron, and cadmium (Cd) transporter. The shoot Cd content in RNAi plants was higher than wild-type (WT) plants, whereas the total (roots plus shoots) lower. For efficient phytoremediation, produced using natural high Cd-accumulating cultivar Anjana Dhan (A5i). Using positron-emitting tracer imaging system, assessed time-course of absorption accumulation A5i plants. Enhanced 107Cd translocation from roots to shoots observed To...
Abstract Genebanks provide access to diverse materials for crop improvement. To utilize and evaluate them effectively, core collections, such as the World Rice Core Collection (WRC) in Genebank at National Agriculture Food Research Organization, have been developed. Because WRC consists of 69 accessions with a high degree genetic diversity, it has used >300 projects. allow deeper investigation existing data further promote research using rice accessions, we performed whole-genome...
Abstract Fertilization controls various aspects of cereal growth such as tiller number, leaf size, and panicle size. However, despite benefits, global chemical fertilizer use must be reduced to achieve sustainable agriculture. Here, based on field transcriptome data from samples collected during rice cultivation, we identify responsive genes focus Os1900 , a gene orthologous Arabidopsis thaliana MAX1 which is involved in strigolactone biosynthesis. Elaborate genetic biochemical analyses...
The complete nucleotide sequence of the tobacco retrotransposon Tto1, one few active retrotransposons plants, was determined. analysis suggests that Tto1 carries all functions required for autonomous transposition through reverse transcription. Gene organization and nature transcription product suggest uses a gene expression mechanism different from those employed by retroviruses most to regulate Gag Pol stoichiometry. introduced into rice study its in heterologous hosts. Transcription were...