A5008268308- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Aluminum toxicity and tolerance in plants and animals
- Multiple Sclerosis Research Studies
- Plant Molecular Biology Research
- Insect Resistance and Genetics
- Rice Cultivation and Yield Improvement
- Plant responses to water stress
- Genetically Modified Organisms Research
- Iron Metabolism and Disorders
- Cassava research and cyanide
- CRISPR and Genetic Engineering
- Plant Parasitism and Resistance
- Agricultural Science and Fertilization
- Plant nutrient uptake and metabolism
- Phytase and its Applications
- Peripheral Neuropathies and Disorders
- Plant tissue culture and regeneration
- Leaf Properties and Growth Measurement
- Nitrogen and Sulfur Effects on Brassica
- Cervical and Thoracic Myelopathy
- GABA and Rice Research
- Plant Genetic and Mutation Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
RIKEN Center for Sustainable Resource Science
2015-2025
The University of Tokyo
2008-2024
Lahore University of Management Sciences
2021-2023
University of Alabama at Birmingham
1999-2023
Federal Urdu University
2019
University of the Punjab
2004-2009
Mayo Hospital
2009
Centre of Excellence in Molecular Biology
2004-2006
Brown University
2004
Creighton University
2004
Metals like manganese (Mn) and iron (Fe) are essential for metabolism, while cadmium (Cd) is toxic virtually all living organisms. Understanding the transport of these metals important breeding better crops. We have identified that OsNRAMP5 contributes to Mn, Fe Cd in rice. expression was restricted roots epidermis, exodermis outer layers cortex as well tissues around xylem. localized plasma membrane complemented growth yeast strains defective transport. RNAi (OsNRAMP5i) plants accumulated...
Rice (Oryza sativa) is indispensable in the diet of most world's population. Thus, it an important target which to alter iron (Fe) uptake and homeostasis, so as increase Fe accumulation grain. We previously isolated OsYSL2, a functional [Fe(II)]- manganese [Mn(II)]-nicotianamine complex transporter that expressed phloem cells developing seeds. produced RNAi (OsYSL2i) overexpression lines (OXOsYSL2) OsYSL2. At vegetative stage OsYSL2i line, Mn concentrations were decreased shoots,...
Heart Disease is the disorder of heart and blood veins. It very difficult for medical practitioners doctors to predict accurate about disease diagnosis. Data science one more important things in early prediction solves large data problems now days. This research paper describes field by using science. As many researches done related that problem but accuracy still needed be improved. So, this focuses on feature selection techniques algorithms where multiple datasets are used experimentation...
Graminaceous plants have evolved a unique mechanism to acquire iron through the secretion of family small molecules, called mugineic acid phytosiderophores (MAs). All MAs are synthesized from l-Met, sharing same pathway l-Met 2'-deoxymugineic (DMA). DMA is reduction 3''-keto intermediate by deoxymugineic synthase (DMAS). We isolated DMAS genes rice (OsDMAS1), barley (HvDMAS1), wheat (TaD-MAS1), and maize (ZmDMAS1). Their nucleotide sequences indicate that OsDMAS1 encodes predicted...
Iron deficiency is one of the major agricultural problems, as 30% arable land world too alkaline for optimal crop production, rendering plants short available iron despite its abundance. To take up apoplasmic precipitated iron, secrete phenolics such protocatechuic acid (PCA) and caffeic acid. The molecular pathways genes uptake strategies are already characterized, whereas mechanisms synthesis secretion have not been clarified, no efflux transporters identified in yet. Here we describe...
Zinc (Zn), an essential nutrient in cells, plays a vital role controlling cellular processes such as growth, development, and differentiation. Although the mechanisms of Zn translocation rice plants (Oryza sativa) are not fully understood, it has recently received increased interest. OsZIP4 is transporter that localizes to apical cells. Transgenic overexpressing gene under control cauliflower mosaic virus (CaMV) 35S promoter were produced. The concentration roots 35S-OsZIP4 transgenic was 10...
In plants, iron (Fe) is essential for mitochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant Fe transporters have not been identified. Here we show, identify characterize the rice transporter (MIT). Based on a transfer DNA library screen, identified line showing symptoms of deficiency while accumulating high shoot levels Fe. Homozygous knockout MIT in this resulted lethal phenotype. localized to mitochondria complemented growth Δmrs3Δmrs4 yeast...
Zinc (Zn) is an essential micronutrient with numerous cellular functions in plants, and its deficiency represents one of the most serious problems human nutrition worldwide. Zn causes a decrease plant growth yield. On other hand, could be toxic if excess amounts are accumulated. Therefore, uptake transport must strictly regulated. In this review, dominant fluxes soil–root–shoot translocation rice plants (Oryza sativa) described, including from soils form Zn2+ Zn-DMA at root surface, to...
Biofortification (increasing the contents of vitamins and minerals through plant breeding or biotechnology) food crops with micronutrient elements has potential to combat widespread deficiencies in humans. Rice (Oryza sativa L.) feeds more than half world's population is used as a staple many parts Asia. As other plants, transport rice controlled at several stages, including uptake from soil, root shoot, careful control subcellular transport, finally, most importantly, seeds. To enhance...
Iron (Fe) is an important micronutrient for living organisms. Fe deficiency severely impairs plant growth and a widespread human dietary problem, with particularly high numbers of affected children females. Rice (Oryza sativa) source energy more than half the world's population. Thus, understanding mechanisms uptake translocation in rice utmost importance development varieties that are tolerant to low availability seed levels Fe. In recent years, underlying transport homeostasis have been...
<ns4:p>Despite recent advancements in plant molecular biology and biotechnology, providing food security for an increasing world population remains a challenge. Drought (water scarcity), salinity, heat, cold stress are considered major limiting factors that affect crop production both qualitatively quantitatively. Therefore, the development of cost-effective environmentally friendly strategies will be needed to resolve these agricultural problems. This require comprehensive understanding...
Drought stress has a negative impact on crop yield. Thus, understanding the molecular mechanisms responsible for plant drought tolerance is essential improving this beneficial trait in crops. In current study, transcriptional analysis was conducted of gene regulatory networks roots soil-grown Arabidopsis plants response to treatment. A microarray drought-stressed and shoots performed at 0, 1, 3, 5, 7, 9 days. Results indicated that expression many stress-responsive genes abscisic acid...
Iron (Fe) is essential micronutrient for plants and its deficiency as well toxicity a serious agricultural problem. The mechanisms of Fe are reasonably understood, however our knowledge about response to excess limited. Moreover, the regulation small open reading frames (sORFs) in abiotic stress has not been reported rice. Understanding rice transcriptome could provide bases developing strategies breed tolerant Fe.We used novel 110 K microarray harbouring ~48,620 sORFs understand...
Iron (Fe) is an essential micronutrient for plants; however, despite its abundance in soils it not readily available neutral to alkaline soils. Plants secrete phenolics, such as protocatechuic acid (PCA) and caffeic (CA), absorb utilize precipitated apoplasmic Fe from root surfaces. However, the synthesis secretion of phenolics have been well characterized plants. We identified a rice (Oryza sativa L.) mutant with reduced amounts PCA CA xylem sap exudates; hence we named efflux zero 2...
Abstract Background The mechanism of iron (Fe) uptake in plants has been extensively characterized, but little is known about how Fe transport to different subcellular compartments affects localization rice seed. Here, we discuss the characterization a vacuolar transporter 2 ( OsVIT2 ) T-DNA insertion line osvit2 and report that knockdown mitochondrial MIT expression seed localization. Findings accumulated less their shoots when grown under normal or excess conditions, while accumulation was...
Manganese (Mn) and iron (Fe) are essential mineral micronutrients for plants their deficiency or toxicity represents a serious agricultural problem. In rice the information about genes involved in Mn uptake from soil is scarce. Recently, we showed that OsNRAMP5 plasma membrane protein Fe transport. The concentration of roots, shoots xylem sap RNAi (OsNRAMP5i) was significantly reduced compared with WT plants. expression not controlled by root also observed pistil, ovary, lemma palea. These...
Zinc (Zn) is an essential micronutrient for living organisms, and understanding the molecular mechanisms of Zn deficiency may help to develop strategies mitigate this problem. Microarray analysis deficient rice revealed up-regulation several genes involved in transport. Moreover many starch synthesis/transport were up-regulated by roots shoots. Furthermore, granules detected mainly cortical cells these tissues. The gene encoding inactive RNase was much more highly transcribed than those...
The Arabidopsis histone deacetylase 6 (HDA6) mutant exhibits increased tolerance to drought stress by negatively regulating the expression of ALDH2B7 and PDC1. Therefore, it was logical determine if transgenic plants expressing PDC1 or using a suitable promoter would also exhibit stress. An analysis published microarray data indicated up-regulation TSPO gene, which encodes an outer membrane tryptophan-rich sensory protein (TSPO), RT-qPCR, as well GUS promoter, confirmed in roots shoots....
Previously, we reported that OsNRAMP5 functions as a manganese, iron, and cadmium (Cd) transporter. The shoot Cd content in RNAi plants was higher than wild-type (WT) plants, whereas the total (roots plus shoots) lower. For efficient phytoremediation, produced using natural high Cd-accumulating cultivar Anjana Dhan (A5i). Using positron-emitting tracer imaging system, assessed time-course of absorption accumulation A5i plants. Enhanced 107Cd translocation from roots to shoots observed To...