A5091221845- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Disease Management Techniques
- Plant Reproductive Biology
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Insect-Plant Interactions and Control
- Light effects on plants
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
- Transgenic Plants and Applications
- Biofuel production and bioconversion
- Plant and animal studies
- Nematode management and characterization studies
- Plant Disease Resistance and Genetics
- Scheduling and Optimization Algorithms
- Genomics and Chromatin Dynamics
- Metaheuristic Optimization Algorithms Research
- Plant Genetic and Mutation Studies
- Genetically Modified Organisms Research
- GABA and Rice Research
Nagoya University
2011-2025
Kyoto University
1999-2018
Nara Institute of Science and Technology
2008-2011
Fujifilm (Japan)
1999
Plant grafting is conducted for fruit and vegetable propagation, whereby a piece of living tissue attached to another through cell-cell adhesion. However, graft compatibility limits combinations closely related species, the mechanism poorly understood. We found that Nicotiana capable adhesion with diverse range angiosperms. Comparative transcriptomic analyses on indicated subclade β-1,4-glucanases secreted into extracellular region facilitates cell wall reconstruction near interface....
Abstract Plasticity of root growth in response to environmental cues and stresses is a fundamental characteristic land plants. However, the molecular basis underlying regulation under stressful conditions poorly understood. Here, we report that rice nuclear factor, RICE SALT SENSITIVE3 (RSS3), regulates cell elongation during adaptation salinity. Loss function RSS3 only moderately inhibits normal conditions, but it provokes spontaneous swelling, accompanied by severe inhibition, saline...
The plant hormone jasmonate and its conjugates (JAs) have important roles in growth control, leaf senescence defense responses against insects microbial attacks. JA biosynthesis is induced by several stresses, including mechanical wounding, pathogen attacks, drought salinity stresses. However, the of JAs under abiotic stress conditions are unclear. Here we report that increased expression Cyt P450 family gene CYP94C2b enhanced viability rice plants saline conditions. This encodes an enzyme...
Nicotiana benthamiana is widely used as a model plant for dicotyledonous angiosperms. In fact, the strains in research are highly susceptible to wide range of viruses. Accordingly, these subject pathology and plant-microbe interactions. terms plant-plant interactions, N. one plants that exhibit grafting affinity with from different families. Thus, good biology has been genome sequencing analyses many years. However, complex allopolyploid genome, its previous reference fragmented into 141,000...
Tissue adhesion between plant species occurs both naturally and artificially. Parasitic plants establish intimate relationship with host by adhering tissues at roots or stems. Plant grafting, on the other hand, is a widely used technique in agriculture to adhere of two Here we found that model Orobanchaceae parasitic Phtheirospermum japonicum can be grafted interfamily species. To understand molecular basis tissue distant species, conducted comparative transcriptome analyses infection...
Abstract In grafting, an agricultural technique for propagating flower species and fruit trees, two plants are combined to exploit their beneficial characteristics, such as rootstock disease tolerance vigor. Grafting incompatibility has been observed, however, between distantly related plant combinations, which limits the availability of resources. A high grafting capacity found in Nicotiana, belonging Solanaceae, but not Ipomoea nil, a Convolvulaceae species. Here, we that Petunia hybrida,...
Abstract VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a transcription factor gene that plays critical role in xylem differentiation. The ectopic expression of VND7 induces the formation secondary cell walls with spiral patterns multiple plant types. In present study, we have identified four homologs Nicotiana benthamiana and assigned them names NbVND7-1 to NbVND7-4. Particularly, NbVND7-2 were highly expressed during N. Arabidopsis thaliana (Nb/At) interfamily grafting. Analysis promoter GUS...
Seed formation is essential for plant propagation and food production. We present a novel mechanism the regulation of seed size by newly identified "gate" at chalazal end ovule regulating nutrient transport into developing seed. This gate blocked callose deposition in unfertilized mature ovules (closed state), but removed after central cell fertilization, allowing (open state). However, if fertilization fails, persists, preventing transportation nutrients from funiculus. A mutant an...
Grafting is an agricultural technique that joins tissues from different plants to obtain useful rootstock traits. However, cellular processes involved in joint tissue repair remain poorly understood. We analyzed Nicotiana benthamiana (Nb) and Arabidopsis thaliana (At) interfamily heterografting as a high-stress model At homografting low-stress model. Transmission electron micrographs reveal the formation of autophagic structures cells near graft boundary over long period Nb/At grafts short...
Environmental stress tolerance is an important trait for crop improvement. In recent decades, numerous genes that confer to abiotic such as salinity were reported. However, the levels of salt differ greatly depending on growth conditions, and mechanisms underlying complicated nature are far from being fully understood. this study, we investigated profiles nine salt-tolerant rice varieties transgenic lines carrying constitutively expressed potentially involved in tolerance, by evaluating...
Plants are exposed to a variety of environmental stress, and starvation inorganic phosphorus can be major constraint in crop production. In plants, response phosphate deficiency soil, miR399, type microRNA (miRNA), is up-regulated. By detecting the early diagnosis stress plants accomplished. However, general miRNA detection methods require complicated experimental manipulations. Therefore, simple rapid required for plant nutritional diagnosis. For microfluidic technology suitable...
SUMMARY Flagellin is a component of bacterial flagella and acts as proteinaceous elicitor defence responses in organisms. from phytopathogenic bacterium, Acidovorax avenae strain N1141, induces immune suspension‐cultured rice cells. To analyse the function flagellin rice, we fused N1141 gene to cauliflower mosaic virus 35S promoter introduced it into rice. Many resulting transgenic plants accumulated at various levels. The developed pale spots leaves. expression defence‐related for...
GIGANTEA (GI), CONSTANS (CO) and FLOWERING LOCUS T (FT) regulate photoperiodic flowering in Arabidopsis. In rice, OsGI, Hd1 Hd3a were identified as orthologs of GI, CO FT, respectively, are also important regulators flowering. Although GI has roles both the circadian clock, our understanding its biochemical functions is still limited. this study, we purified novel OsGI-interacting proteins by using tandem affinity purification (TAP) method. The TAP method been used effectively a number model...
In a determinate meristem, such as floral genetically determined number of organs are produced before the meristem is terminated. rice, iterative formation during flower development with defects in determinacy, classically called 'proliferation', caused by several mutations and observed dependence on environmental conditions. Here we report that overexpression JAZ proteins, key factors jasmonate signaling, Jas domains causes an increase numbers florets, aberrant patterns organ repetitious...
Grafting is an indispensable agricultural technology for propagating useful tree varieties and obtaining beneficial traits of two varieties/species—as stock scion—at the same time. Recent studies molecular events during grafting have revealed dynamic physiological transcriptomic changes. Strategies focused on specific steps are needed to further associate each event with those steps. In this study, we developed a method investigate tissue adhesion event, early step, by improving artificial...
Abstract Grafting is a plant propagation technique widely used in agriculture. A recent discovery of the capability interfamily grafting Nicotiana has expanded potential combinations grafting. In this study, we showed that xylem connection essential for achievement and investigated molecular basis formation at graft junction. Transcriptome gene network analyses revealed modules tracheary element (TE) during include genes associated with cell differentiation immune response. The reliability...
In many plants, timing of flowering is regulated by day length. Arabidopsis, florigen, FLOWERING LOCUS T (FT) protein, synthesized in leaf phloem companion cells response to long days and transported the shoot apical meristem (SAM) through phloem. The temporal aspects florigen transportation have been studied various plants physiological experiments. Nevertheless, little known about how FT protein Arabidopsis. this study, we performed heat shock-based transient induction a single blade...
Abstract Carbohydrates (sugars) are an essential energy-source for all life forms. They take a significant share of our daily consumption and used biofuel production as well. However, sugarcane sugar beet the only two crop plants which to produce in amounts. Here, we have discovered fine-tuned phenomenon rice leads them sugary-grain. We knocked-out GCS1 genes by using CRISPR technology, led fertilization failure pollen tube-dependent ovule enlargement morphology (POEM) phenomenon....
The tobacco BY-2 cell line has been used widely as a model system in plant biology. cells are nearly transparent, which facilitates imaging using fluorescent markers. As cultured drifted the medium, therefore, it was difficult to observe them for long period. Hence, we developed microfluidic device that traps and fixes their positions allow monitoring physiological activity of cells. contains 112 trap zones, with parallel slots connected series at three levels flow channel. were 7 days...
Plant grafting is generally performed between closely related species. Recently, we have discovered that Nicotiana species of Solanaceae show the ability to graft with distantly plant beyond family. Graft adhesion diverse angiosperms by was probably facilitated secretion a subclade ß-1,4-glucanases. The capability interfamily also found in model Orobanchaceae hemiparasitic plant, Phtheirospermum japonicum, which naturally invades tissues host plants different families. Transcriptome analysis...
Summary Grafting is an important technique in agriculture to obtain several good traits such as high disease tolerance and yield by exchanging root system. However, the underlined cellular processes compensate wound damage repair tissues were largely unknown. We analyzed two graft combinations: Nicotiana benthamiana ( Nb ) homograft a compatible, repairing model heterograft with Arabidopsis thaliana At incompatible more stressful model, which we recently identified exceptional maintainable...