A5066515866- 3D Printing in Biomedical Research
- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Magnesium Alloys: Properties and Applications
- Aluminum Alloys Composites Properties
- Neuroscience and Neural Engineering
- Protein Hydrolysis and Bioactive Peptides
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Chemical Synthesis and Analysis
- Cellular Mechanics and Interactions
- Metal and Thin Film Mechanics
- Mesenchymal stem cell research
- Microbial Metabolic Engineering and Bioproduction
- Advanced Sensor and Energy Harvesting Materials
- Bone Tissue Engineering Materials
- Adipose Tissue and Metabolism
- Antimicrobial Peptides and Activities
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Advanced Wireless Communication Techniques
- Error Correcting Code Techniques
- Aluminum Alloy Microstructure Properties
- Virus-based gene therapy research
Nagoya University
2016-2025
Tateyama Elementary School
2019-2024
Nagoya University Hospital
2021-2022
Graduate School USA
2021
Osaka Prefectural Medical Center
2010-2020
Daiichi Sankyo (Germany)
2016-2019
Kyoto Pharmaceutical University
2012-2017
Kyoto University
2010-2017
Ritsumeikan University
2010-2017
Nagaoka University of Technology
2015
Abstract An in vitro reconstruction of three‐dimensional (3D) tissues without the use scaffolds may be an alternative strategy for tissue engineering. We have developed a novel engineering strategy, termed magnetic force‐based (Mag‐TE), which magnetite cationic liposomes (MCLs) with positive charge at liposomal surface, and force were used to construct 3D scaffolds. In this study, human mesenchymal stem cells (MSCs) magnetically labeled MCLs seeded onto ultra‐low attachment culture magnet...
Alignment of cells plays a significant key role in skeletal muscle tissue engineering because vivo has highly organized structure consisting long parallel multinucleated myotubes formed through differentiation and fusion myoblasts. In the present study, we developed an easy, simple, low-cost method for aligning by using surfaces with linear microscale features fabricated grinding. Iron blocks were ground one direction three kinds abrasives (9 microm diamond suspension, #400 sandpaper, #150...
Objective— Therapeutic angiogenesis with cell transplantation represents a novel strategy for severe ischemic diseases. However, some patients have poor response to such conventional injection-based angiogenic therapy. Here, we investigated therapeutic potential of mesenchymal stem (MSC) sheet created by magnetite tissue engineering technology reparative angiogenesis. Methods and Results— Human MSCs incubated magnetic nanoparticle-containing liposomes were cultured, magnet was placed on the...
Abstract To engineer functional tissues, a large number of cells must be successfully seeded into scaffolds. We previously proposed methodology for tissue engineering using magnetite nanoparticles and magnetic force, which we termed “Mag‐TE.” In the present study, applied Mag‐TE technique to cell seeding process have “Mag‐seeding.” The cell‐seeding efficiency NIH/3T3 fibroblasts (FBs) by Mag‐seeding was investigated six types commercially available scaffolds (5 collagen sponges 1 D , ‐ L...
Abstract Heart tissue engineering requires construction of three‐dimensional (3‐D) tissues composed cardiomyocytes (CMs) that are tightly connected to each other. The aim this study was construct “scaffold‐less” multi‐layered 3‐D CM sheets using magnetic force‐based (Mag‐TE) and evaluate the cell‐to‐cell functional connections within sheets. Original magnetite cationic liposomes (MCLs) with a positive surface charge (which facilitate adsorption target cell surface) were taken up by CMs...
Although cell sheet tissue engineering is a potent and promising method for engineering, an increase of mechanical strength needed easy manipulation it during transplantation or 3D fabrication. Previously, we developed sheet-polymer film complex that had enough can be manipulated even by tweezers (Fujita et al., 2009. Biotechnol Bioeng 103(2): 370-377). We confirmed the polymer involving temperature sensitive extracellular matrix (ECM) proteins could removed lowering after transplantation,...
Drug screening systems for muscle atrophy based on the contractile force of cultured skeletal tissues are required development preventive or therapeutic drugs atrophy. This study aims to develop a model by inducing in normal constructed microdevices capable measuring and verify if this is suitable drug using as an index. Tissue engineered muscles containing striated myotubes were prepared study. The addition 100 µM dexamethasone (Dex), which used inducer, 24 h reduced significantly. An...
Abstract Pathophysiological analysis and drug discovery targeting human diseases require disease models that suitably recapitulate patient pathology. Disease-specific induced pluripotent stem cells (hiPSCs) differentiated into affected cell types can potentially pathology more accurately than existing models. Such successful modeling of muscular requires efficient differentiation hiPSCs skeletal muscles. transduced with doxycycline-inducible MYOD1 ( -hiPSCs) have been widely used; however,...
Abstract This study aimed to obtain angiotensin converting enzyme (ACE) inhibitory peptides from barley hordein, a protein rich in proline and glutamine, identify novel inhibitors. Hordein was hydrolyzed using four enzymes: pepsin, trypsin, papain, orientase, an Aspergillus oryzae known cleave the C-terminal side of residues. The hydrolysate orientase showed strong ACE activity. LC-MS/MS analysis identified 35 peptides, including 23 derived hordein 9 After synthesizing 15 7 significant...
Background The heart is a complex organ composed of diverse cell types, whose interplay crucial for development, tissue homeostasis, and disease progression. A potential approach to recapitulate this heterotypic multicellular environment the co-differentiation induced pluripotent stem cells (iPSCs) into multiple cardiac facilitating unlocking their full regenerative medicine drug development. However, inherent complexity co-differentiation, where differentiation factors simultaneously...
We have compared several serum-free media for the differentiation of C2C12 myoblasts and assessed extent in ways including as to active tension generation capability. cells were allowed differentiate Dulbecco's modified Eagle's medium (DMEM) containing Ham's F-12 (F-12), AIM-V (AIM), 0.2% Ultroser-G DMEM (Ult-G), 0.1% Sericin (Sericin), with supplemented 2% horse serum (HS) or calf (CS). was myotube formation, glucose metabolism, protein expression, sarcomere generation. All examined capable...
This paper reports a novel combination of hydrophilic/hydrophobic materials for the evolution liquid manipulation. Droplet generation based on mechanism is promising method highly accurate manipulations. Although several droplet manipulation devices utilizing patterns have been reported, it has difficult to split fluid into droplets solely through in microchannel. In this study, material fabricating was investigated and their wettability difference enhanced generation. To improve...
Charge-neutralized lipid envelope-type nanoparticles formed with SS-cleavable and pH-activated lipid-like materials (ssPalm) accumulate rapidly in the liver without forming aggregates blood circulation, result a liver-specific gene expression for long duration (>2 weeks) neither immunological responses nor hepatotoxicity after intraveneous administration, when it carries pDNA free from CpG-motifs.