A5072020921- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Animal Genetics and Reproduction
- Monoclonal and Polyclonal Antibodies Research
- Liver physiology and pathology
- Tissue Engineering and Regenerative Medicine
- Protein purification and stability
- RNA Interference and Gene Delivery
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Microfluidic and Bio-sensing Technologies
- Muscle Physiology and Disorders
- Nanoparticle-Based Drug Delivery
- Glycosylation and Glycoproteins Research
- Chemical and Physical Properties in Aqueous Solutions
- Cellular Mechanics and Interactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- Microfluidic and Capillary Electrophoresis Applications
Kyushu University
2016-2025
KM Biologics (Japan)
2022
ECSI Fibrotools (United States)
2008-2015
Nagoya University
2001-2013
Lund University
1992-2002
The sterilizing effect of supercritical carbon dioxide (SC-C02) is described as compared with gaseous C02(G-C02) and liquid C02(L-C02). Baker's yeast, Escherichia coli, Staphylococcus aureus conidia Aspergillus niger were sterilized by treating SC-C02 at 200 atm 35°C when the water content each microorganism was 70 ~ 90%. However, dry cells a 2 10% could not be under same conditions. No from on endospores Bacillus subtilis B. s tear other mophilus observed. G-C02 L-C02 produced no against...
Electrical impulses are necessary for proper in vivo skeletal muscle development. To fabricate functional tissues vitro, recapitulation of the niche, including physical stimuli, is crucial. Here, we report a technique to engineer vitro by electrical pulse stimulation (EPS). Electrically excitable tissue-engineered constructs were stimulated with continuous pulses 0.3 V/mm amplitude, 4 ms width, and 1 Hz frequency, resulting 4.5-fold increase force at day 14. In myogenic differentiation...
The sterilizing effect of supercritical carbon dioxide (SC-CO2) is described as compared with gaseous CO2(G-CO2) and liquid CO2(L-CO2). Baker's yeast, Escherichia coli, Staphylococcus aureus conidia'of Aspergillus niger were sterilized by treating SC-CO2 at 200 atm 35°C when the water content each microorganism was 70-90%. However, dry cells a 2-10% could not be under same conditions. No from on endospores Bacillus subtilis B. stearothermophilus observed. G-CO2 L-CO2 produced no against both...
We report here the generation of transgenic chickens using a retroviral vector for production recombinant proteins. It was found that transgene expression suppressed when Moloney murine leukemia virus-based injected into chicken embryos at blastodermal stage. When concentrated viral solution heart developing after 50 to 60 h incubation, observed throughout embryo, including gonads. For practical production, encoding an cassette antiprion single-chain Fv fused with Fc region human...
Gene knock-in techniques have rapidly evolved in recent years, along with the development and maturation of genome editing technology using programmable nucleases. We recently reported a novel strategy for microhomology-mediated end-joining-dependent integration donor DNA by TALEN or CRISPR/Cas9 optimized targeting vectors, named PITCh (Precise Integration into Target Chromosome) vectors. Here we describe vector-mediated long gene cassettes, including single-chain Fv-Fc (scFv-Fc) gene,...
Recent progress in the use of decellularized organ scaffolds as regenerative matrices for tissue engineering holds great promise addressing issue donor shortage. Decellularization preserves mechanical integrity, composition, and microvasculature critical zonation hepatocytes liver. Earlier studies have reported possibility repopulating with hepatic cell lines or stem cells to improve liver regeneration. In this work, we study versatility matrix a substrate coating three-dimensional cryogel...
Skeletal muscle tissue engineering is currently applied in a variety of research fields, including regenerative medicine, drug screening, and bioactuator development, all which require the fabrication biomimic functional skeletal tissues. In present study, magnetite cationic liposomes were used to magnetically label C2C12 myoblast cells for construction three-dimensional artificial tissues by an magnetic force. functions, such as biochemical contractile properties, evaluated constructs....
With a view to the application of magnetic particles in cancer thermotherapy (hyperthermia), methods preparing bio-applicable magnetite with targeting activity towards cells were investigated, and properties material examined. Poly(ethylene glycol) (PEG)-magnetite consisting (Fe3O4) PEG terminal carboxy or amino groups was prepared. Monoclonal antibody then immobilized covalently on PEG-magnetite. Among three different immobilization employed, highest density 492 mg protein/g PEG-magnetite...
The capture of human acute myeloid leukemia KG-1 cells expressing the CD34 surface antigen and fractionation blood lymphocytes were evaluated on polyvinyl alcohol (PVA)–cryogel beads dimethyl acrylamide (DMAAm) monolithic cryogel with immobilized protein A. affinity ligand (protein A) was chemically coupled to reactive PVA–cryogel epoxy-derivatized cryogels through different immobilization techniques binding efficiency cell receptors specific antibody-labeled gels/beads determined. higher...
Abstract Aqueous two‐phase extraction incorporated affinity precipitation was examined as a technique for protein purification. An enteric coating polymer, Eudragit S100, employed ligand carrier. specifically partitioned to the top phase in aqueous systems. For application of this method purification recombinant A using human IgG coupled an system, 80% added recovered with 81% purity. The purity enhanced 26‐fold by thid method. IgG‐Eudragit could be used repeatedly process. This seperation...
Genomics-based safety and quality evaluation studies are advancing the bioindustry by enhancing various aspects, including viral safety, host cell protein (HCP) control, product heterogeneity cellular process reproducibility. High-throughput instruments genome-scale databases essential in genomics, with reference genome sequence being most critical database. The completeness accuracy of these sequences depend on DNA quality, sequencing instruments, read coverage, assembly strategies....
Abstract A new type of aqueous two‐phase system (ATPS) has been developed in which a temperature‐sensitive polymer, poly‐ N ‐isopropylacrylamide [poly (NIPAM)] was used as ligand carrier for the specific separation animal cells. Monoclonal antibodies were modified with itaconic anhydride and copolymerized ‐isopropylacrylamide, ligand‐conjugated carriers added to polyethylene glycol 8000–dextran T500 systems. The antibody–polymer conjugates partitioned top phase absence or presence 0.15 M...
The Cre-loxP system is frequently used for site-specific recombination in animal cells. equilibrium and specificity of the reaction can be controlled using mutated loxPs. In present study, we designed an accumulative gene integration Cre recombinase loxPs which Cre-mediated cassette exchange infinitely repeatable target into loxP sites. To evaluate feasibility usefulness this system, a series reactions were repeated confirmed vitro protein plasmids. Accumulative was also performed on genome...